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Identification of Uncommon PIK3CA Mutations in Lung Cancer by Using Pyrosequencing

Schildgen, Verena PhD*; Lüsebrink, Jessica PhD*; Appel, Jan D.*; Wübben, Christine*; Engel-Riedel, Walburga MD; Ludwig, Corinna MD; Stoelben, Erich MD; Schildgen, Oliver PhD*; Brockmann, Michael MD*

Diagnostic Molecular Pathology: March 2013 - Volume 22 - Issue 1 - p 22–27
doi: 10.1097/PDM.0b013e31825f5f93
Original Articles
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Introduction: Phospatidylinositol-3-kinases (PI3K) play an important role in various cell processes. Oncogenic mutations in the PIK3CA gene, which codes for the catalytic subunit, have been identified in various malignancies and activate the PI3K/AKT/mTOR pathway, which is a critical driver of tumorigenesis.

Methods: We tested 41 tumor samples with known KRAS, BRAF, and EGFR mutation status for the occurrence of mutations in the PIK3CA gene, using a pyrosequencing assay.

Results: Pyrosequencing revealed 2 mutations (4.9%) in the PIK3CA gene, one in exon 9 and the other in exon 20. Both mutations have not been identified yet in lung tumor tissue.

Discussion: The screening of our small patient cohort by pyrosequencing identified 2 mutations (4.9%) in PIK3CA, one in exon 9 (Q546H) and the other in exon 20 (M1043T). Both mutations have not been described in lung tumors yet and seem to be rather uncommon mutations. Future screening of large patient cohorts with pyrosequencing may contribute to the detection of more mutations in lung cancer because of the low limit of detections of this method and may contribute to a better understanding of the interaction of mutations and tumorigenesis.

*Institut für Pathologie

Lungenklinik, Kliniken der Stadt Köln gGmbH, Köln, Germany

V.S. and J.L. contributed equally.

The authors declare no conflict of interest.

Reprints: Michael Brockmann, MD, Kliniken der Stadt Köln gGmbH, Krankenhaus Merheim, Klinikum der Privaten Universität Witten/Herdecke, Institut für Pathologie, Ostmerheimer Str. 200, D-51109 Köln (Cologne), Germany (e-mail: brockmannm@kliniken-koeln.de).

© 2013 Lippincott Williams & Wilkins, Inc.