Detection of Transplacental Melanoma Metastasis Using Quantitative PCRRaso, Alessandro PhD*; Mascelli, Samantha PhD*; Nozza, Paolo MD†; Biassoni, Roberto PhD‡; Negri, Francesca PhD†; Garaventa, Alberto MD§; Tarantino, Vincenzo MD∥; Garrè, Maria Luisa MD¶; Cama, Armando MD*; Capra, Valeria MD*Author Information *Neurosurgery Unit †Pathology Unit ‡Molecular Medicine Unit ¶Neuro-Oncology Unit Departments of §Haematology-Oncology ∥Otolaryngology, Giannina Gaslini Children's Research Hospital, Genoa, Italy Reprints: Alessandro Raso, PhD, Neurosurgery Unit, Giannina Gaslini Children's Research Hospital, Genoa, Italy. Largo Gerolamo Gaslini 5, Genoa 16147, Italy (e-mail: [email protected]). Research was performed at the Neurosurgery Unit, Giannina Gaslini Children's Research Hospital, Genoa, Italy. The authors declare no conflict of interest. Diagnostic Molecular Pathology: June 2010 - Volume 19 - Issue 2 - pp 78-82 doi: 10.1097/PDM.0b013e3181b6a546 Buy Metrics Abstract To choose the most appropriate treatment for children affected by a transplacental metastasis, it is crucial to ascertain the maternal origin of the tumor. Up-to-date conclusive diagnosis is generally achieved through fluorescence in situ hybridization or karyotyping analysis. Herein, we report an alternative, reliable assay for rapidly defining vertical cancer transmission to the fetus by using quantitative polymerase chain reaction. Our assay indicates that quantification of the copy number of the sex chromosomes by specific short tandem repeats markers, in genomic DNA purified from the tumor biopsy cells, could be used to correctly evaluate transplacental metastasis events. © 2010 Lippincott Williams & Wilkins, Inc.