We conducted a case-control study to investigate the associations of functional single-nucleotide polymorphisms in the purinergic P2X7 receptor (P2X7R) gene (rs2393799, rs7958311, rs1718119, rs2230911, and rs3751143) with obesity and overweight in a population of Chinese postmenopausal women.
Our study included 180 obese women, 179 overweight women, and 204 controls. All participants were genotyped at the P2X7R rs2393799, rs7958311, rs1718119, rs2230911, and rs3751143 loci via allele-specific polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism procedures. The relationships between P2X7R genetic polymorphisms and their associated haplotypes with obesity (body mass index [BMI] ≥30 kg/m2] and overweight (25 kg/m2 ≤ BMI < 30 kg/m2) were evaluated.
Our results showed that P2X7R rs2230911G and rs1718119A were associated with an increased risk of obesity; in particular, both carriers of the rs2230911G allele and GG/(CG + GG) genotypes (G vs C, P < 0.001, odds ratio [OR] 2.87, 95% confidence interval [CI] 1.98-4.16; GG vs CC, P < 0.001, OR 8.76, 95% CI 3.29-23.35; CG + GG vs CC, P < 0.001, OR 2.54, 95% CI 1.63-3.95) and carriers of the rs17181191A allele and GA/(GA + AA) genotypes (A vs G, P < 0.001, OR 2.97, 95% CI 1.86-4.74; GA vs GG, P = 0.001, OR 2.72, 95% CI 1.55-4.79; GA + AA vs GG, P < 0.001, OR 3.05, 95% CI 1.79-5.19) were at a higher risk of obesity. No association with obesity or overweight was observed for the other three P2X7R polymorphisms (rs2393799, rs7958311, and rs3751143). Haplotype analysis indicated that P2X7R rs1718119A-rs2230911G-rs3751143C appeared to be a significant risk haplotype with obesity (P = 0.0005, OR 2.37, 95% CI 1.45-3.90).
P2X7R functional genetic polymorphisms and their estimated haplotypes are associated with obesity in Chinese postmenopausal women.
1Undergraduate Student of Clinical Medical College, JiangXi Medical College of Nanchang University, Nanchang, Jiangxi, P.R. China
2Department of Physiology, JiangXi Medical College of Nanchang University, Nanchang, Jiangxi, P.R. China
3Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi, P.R. China
4Department of Science and Education, Chest Hospital of Jiangxi Province, Nanchang, Jiangxi, P.R. China
5Undergraduate student of School of Public health, JiangXi Medical College of Nanchang University, Nanchang, Jiangxi, P.R. China
6The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China
7The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China.
Address correspondence to: Hong Xu, MD, Department of Physiology, JiangXi Medical College of Nanchang University, Nanchang, Jiangxi 330006, P.R. China. E-mail: firstname.lastname@example.org
Received 16 July, 2017
Revised 31 August, 2017
Accepted 31 August, 2017
SR is the joint first author.
Funding/support: This work was supported by the grants (Nos. 81760595, 81560529, and 81302501) from National Natural Science Foundation of China, the grants (Nos. 20171ACB21003 and 20161BAB205209) from Natural Science Foundation of Jiangxi Province, the grant (No. 20151BBG70249) from Technology Pedestal and Society Development Project of Jiangxi Province, the grant (No. 20162BCB23021) from the Outstanding Young Scientist Training Object of Jiangxi Province, the grants (Nos. GJJ14093) from Education Department of Jiangxi Province, the grant (Nos. 20151332 and 20155643) from the Foundation of the Health Department of Jiangxi Province, and the grant (Nos. 201510403039, 2015195, 201710403039, cx2016279, 2016211, 1326, 201410403132, 20140611, 201510403035, 20151122040105, 2015191, and 14001840) from Nanchang University Students’ innovation and entrepreneurship training program.
Financial disclosure/conflicts of interest: None reported.
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