From the 1Monash Institute of Medical Research–Prince Henry’s Institute of Medical Research, Clayton, Victoria, Australia; 2Department of Obstetrics and Gynecology, Monash University, Clayton, Victoria, Australia; 3Ansh Labs, Webster, TX; 4Department of Obstetrics, Gynecology, and Reproductive Sciences, College of Medicine, University of Saskatchewan, Saskatchewan, Canada; and 5Department of Internal Medicine, Erasmus MC, Rotterdam, the Netherlands.
Received January 1, 2014; revised and accepted March 4, 2014.
Funding/support: Ansh Labs provided the Monash Institute of Medical Research–Prince Henry’s Institute of Medical Research (MIMR-PHI) with the 24/32 and 24/37 AMH ELISA kits at cost. Beckman Coulter (Chaska, MN) provided hormone assays. This study was supported by research grants from the National Health and Medical Research Council of Australia (program grant 494802, research fellowship (#169201) to D.M.R. and by the Victorian Government’s Operational Infrastructure Support Program (PHI data audit number 13:42).
Financial disclosure/conflicts of interest: This study was undertaken as a collaboration of the MIMR-PHI, University of Saskatchewan (Department of Obstetrics, Gynecology, and Reproductive Sciences), Erasmus University, and Ansh Labs to develop an antimüllerian hormone (AMH) enzyme-linked immunosorbent assay (ELISA) kit that is suitable for detecting serum AMH levels in the menopausal transition. Ansh Labs developed the monoclonal antibodies and the two AMH ELISA formats. Researchers at the University of Saskatchewan and Erasmus University were involved in sample collection and aspects of antibody selection, respectively. Researchers at the MIMR-PHI were involved in assay design and in the planning, undertaking, and writing of this study. Ansh Labs provided methodologies related to the 24/32 and 24/37 AMH ELISAs and reviewed the manuscript. A.P.T. serves as a consultant to Ansh Labs. All the other authors have no conflicts of interest or relevant financial interests other than those stated above.
Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s Web site (www.menopause.org).
Address correspondence to: David M. Robertson, PhD, MIMR-PHI Institute of Medical Research, 27-31 Wright St, Clayton, Victoria 3168, Australia. E-mail: [email protected]
