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Hyaluronic acid concentration in postmenopausal facial skin after topical estradiol and genistein treatment: a double-blind, randomized clinical trial of efficacy

Patriarca, Marisa Teresinha MD, PhD1; Barbosa de Moraes, Andréa Regina MD, PhD1; Nader, Helena B. PhD2; Petri, Valeria MD, PhD3; Martins, João Roberto Maciel MD, PhD2,4; Gomes, Regina Célia Teixeira PhD5; Soares, José Maria Jr MD, PhD1

doi: 10.1097/gme.0b013e318269898c
Original Articles

Objective The aim of this work was to compare the effects of estradiol and genistein treatment on hyaluronic acid (HA) concentration in postmenopausal facial skin.

Methods In this study, 30 postmenopausal women were evaluated in a prospective, randomized, double-blind trial. The volunteers were postmenopausal women treated in the Gynecology Department of the Federal University of São Paulo. The participants were divided into two groups: group E, treated with 0.01% 17β-estradiol gel (n = 15), and group G, treated with 4% genistein gel (isoflavones, n = 15). The treatment lasted for 24 consecutive weeks. Preauricular skin biopsies were performed for each participant at baseline (E1 and G1) and after treatment (E2 and G2) to evaluate HA concentration in tissue. The materials were processed using immunohistochemical and biochemical methods.

Results After 24 weeks of treatment, HA concentration increased in both groups, but the effect was greater for estradiol treatment than for genistein treatment.

Conclusions Our data suggest that both treatments may enhance HA concentration in postmenopausal skin but that estrogen produces results that are greater than those produced by isoflavones.

From the 1Department of Gynecology, 2Molecular Biology Division, Department of Biochemistry, 3Department of Dermatology, 4Endocrinology Division, Department of Internal Medicine, and 5Department of Morphology and Genetics, Federal University of São Paulo-Escola Paulista de Medicina, São Paulo, Brazil.

Received May 1, 2012; revised and accepted July 12, 2012.

Marisa Teresinha Patriarca and Andréa Regina Barbosa de Moraes contributed equally to this work.

Funding/support: This study was supported by Consejo Nacional de Desarrollo Científico y Tecnológico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, and Funda cão de Amparo a Pesquisa do Estado de São Paulo (2011/12417-8).

Financial disclosure/conflicts of interest: None reported.

Address correspondence to: José Maria Soares Jr, MD, PhD, Gynecology Department, Universidade Federal de São Paulo, Apt 11-04021-001, Street Sena Madureira, 1245, Brazil. E-mail:

© 2013 by The North American Menopause Society.