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Influence of marketed herbal menopause preparations on MCF-7 cell proliferation

Bodinet, Cornelia PhD; Freudenstein, Johannes PhD

doi: 10.1097/01.GME.0000094209.15096.2B
Articles

Objective: Given the increasing use of alternative menopause treatments, we evaluated the effect of several herbal preparations used for menopause relief on the proliferation of estrogen-sensitive breast cancer cells (MCF-7) as a means of assessing appropriateness for use in women at risk for estrogen-sensitive breast cancer.

Design: An MCF-7 cell culture model, as described previously, 1 was used to evaluate the estrogen-agonist and -antagonist activity of commercially available herbal menopause preparations containing red clover, soy, black cohosh, or a combination of herbs. Each test substance was evaluated for cytotoxic effects before conducting the proliferation assays.

Results: Commercially available products containing soy, red clover, and herbal combinations induced an increase in the MCF-7 proliferation rates, indicating an estrogen-agonistic activity in the absence of estradiol. In contrast, an isopropanolic black cohosh extract (Remifemin Menopause) did not stimulate MCF-7 growth and exerted inhibitory effects on cellular proliferation. None of the tested products enhanced estradiol-induced cell proliferation. The black cohosh preparation and one of the herbal combinations exhibited strong estrogen-antagonistic effects.

Conclusions: The lack of proliferative effects of isopropanolic black cohosh extract on estrogen-sensitive breast cancer cells in vitro suggests a favorable safety profile for use in women with a history of breast cancer. Alternatively, preparations containing red clover, soy, and combinations of various herbal ingredients may induce cell proliferation, suggesting that such herbal preparations should be used with caution in the treatment of menopause symptoms in women at risk for, or with a history of, estrogen-sensitive breast cancer.

The estrogen-agonist and -antagonist activity of commercially available herbal menopause preparations containing red clover, soy, black cohosh, or a combination of herbs was evaluated in a human estrogen-sensitive breast cancer (MCF-7) cell culture model. Products containing soy, red clover, and herbal combinations induced an increase in the MCF-7 proliferation rates, indicating an estrogen-agonistic activity in the absence of estradiol; whereas, in contrast, an isopropanolic black cohosh extract did not stimulate MCF-7 growth and exerted inhibitory effects on cellular proliferation. None of the tested products enhanced estradiol-induced cell proliferation

Schaper & Brümmer GmbH & Co. KG, Research and Development Department, Salzgitter, Germany

Received March 10, 2003; revised and accepted August 13, 2003.

Address correspondence to: Cornelia Bodinet, PhD, Bahnhofstr. 35, 38259 Salzgitter, Germany. E-mail: cornelia.bodinet@schaper-bruemmer.de.

©2004The North American Menopause Society