P7 Clinical in vivo high-resolution multiphoton tomography of human skin for imaging cancer cells, extracellular matrix components, and sunscreen nanoparticles
Clinical non-invasive multiphoton tomography with subcellular resolution based on two-photon autofluorescence, second-harmonic generation and fluorescence-lifetime imaging has been used for imaging of cancer cells of human skin as well as extracellular matrix components, and measuring the diffusion depth of sunscreen nanoparticles. The multiphoton tomographs DermaInspectTM and MPTflex(tm) (JenLab) are novel skin imaging devices using a near-infrared femtosecond laser source. Non-linear excitation allows to detect natural endogenous fluorophores inside human skin such as NAD(P)H, flavins, elastin, collagen, melanin, keratin, and porphyrins down to a tissue depth of 200 μm without applying any contrast agents. Individual intratissue cells, intracellular mitochondria, melanosomes, and the morphology of the nuclei as well as extracellular matrix elements have been imaged. So far, more than 1000 patients and volunteers in Europe, Asia, and Australia have been investigated with this novel molecular imaging tool for the early diagnosis of malignant melanoma, dermatitis, and actinic keratosis as well as to trace sunscreen nanoparticles, anti-aging drugs, and pharmaceutical components.© 2010 Lippincott Williams & Wilkins, Inc.