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P59 Poly(ADP-ribosylation) and Chromatin assembly factor-1 influence chromatin replication and biological aggressiveness of skin and mucosal squamous cell carcinomas of the head and neck

Siano, M.; Mascolo, M.; Ilardi, G.; Nugnes, L.; Vecchione, M.A.; De Rosa, G.; Staibano, S.

doi: 10.1097/01.cmr.0000382892.20350.89
NMSC I: Epidemiology – Genetics – Clinical Aspects

Department of Biomorphological and Functional Sciences, Pathology Section, University Federico II of Naples, School of Medicine, Naples, Italy

Poly(ADP-ribosylation) is a major post-translational (epigenetic) modification involving a large number of proteins of eukaryotic cells. Several lines of evidence indicate that the Poly(ADP-ribosylation) of histones, beyond the well-known activity in DNA strand breaks repair, plays a pivotal role also in chromatin structure, replication and assembly [1–2].

A critical role in chromosome assembly and replication has been shown also for the Chromatin Assembly Factor (CAF)-1 complex, which expresses at high level its p60 subunit whenever a hyperproliferative condition takes place in eukaryotic cells [3–4].

We examined the immunohistochemical expression of PARP-1 and CAF-1/p60 in a selected series of squamous cell carcinomas (SCC) of the head and neck region, to evaluate its relationship with the clinical and pathological features of tumors.

The results of the study support the existence of a statistically significative correlation between the overexpression of both the proteins and the worse prognosis of patients, outlining the role of epigenetic events in the gain of aggressiveness of skin and mucosal SCC, and the role of PARP-1 and CAF-1/p60 as specific promising targets for molecular therapies for SCC.

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