ORIGINAL ARTICLES: Basic researchPotentiated cytotoxic effects of statins and ajoene in murine melanoma cellsLedezma, Eliadesa; Wittig, Olgab; Alonso, Joseb; Cardier, Jose E.bAuthor Information aFundacite, Estado Trujillo bLaboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado, Caracas, Venezuela Correspondence to Jose E. Cardier, MD, PhD, Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado Postal 21827, Caracas 1020A, Venezuela Tel: +58 212 5041263 x1158; fax: +58 212 5041086; e-mail: [email protected] Received 1 April 2008 Accepted 1 October 2008 Melanoma Research: April 2009 - Volume 19 - Issue 2 - p 69-74 doi: 10.1097/CMR.0b013e32831bc45a Buy Metrics Abstract Because statins and ajoene inhibit the 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, we evaluated the hypothesis that the cytotoxic effect of these compounds may be potentiated when both are used in combination on tumor cells. We showed that cotreatment of the murine melanoma B16F10 cell with statins (atorvastatin and pravastatin) and ajoene, all at nontoxic doses, dramatically increased their cytotoxicity. B16F10 cell death induced by statins, but not by ajoene, was prevented by mevalonate and geranylgeranylpyrophosphate. To our knowledge, this is the first report that the combination of statins and ajoene, which alters the mevalonate pathway, might potentiate their cytotoxic effects on tumor cells. © 2009 Lippincott Williams & Wilkins, Inc.