SHORT COMMUNICATIONThe effect of blue light exposure and use of intraocular lenses on human uveal melanoma cell linesMarshall, Jean-Claude A.; Gordon, Keith D.; McCauley, Cristin S.; de Souza Filho, João Pessoa; Burnier, Miguel N.Author Information Henry C. Witelson Ocular Pathology Laboratory and Registry, McGill University, Montreal, Quebec, Canada Correspondence and requests for reprints to Mr Jean-Claude A. Marshall, MSc, 3775 University Street, Lyman Duff Building, Room 216, Montreal, Quebec, Canada H3A 2B4 Tel: +1 514 398 7192 ext. 00384; fax: +1 514 398 5728; e-mails: [email protected][email protected] Sponsorship: This research is supported by a grant from the Cedars Cancer Institute of Canada. Intraocular lenses supplied by Alcon Canada. Received 17 February 2006 Accepted 11 September 2006 Melanoma Research: December 2006 - Volume 16 - Issue 6 - p 537-541 doi: 10.1097/CMR.0b013e3280112b86 Buy Metrics Abstract Little is known about the effect of blue light on inducing melanocytic malignant transformation. We chose to investigate the effect of blue light (475 nm wavelength) on the proliferation rates of uveal melanoma cells. In addition, we tested two different intraocular lenses to determine the possible effects of ultraviolet absorbing and blue light filtering intraocular lenses on the changes in proliferation. Four human uveal melanoma cell lines (92.1, MKT-BR, OCM-1, SP6.5) were exposed to blue light with and without the presence of ultraviolet absorbing and blue light filtering intraocular lenses. Cells covered by aluminum foil were used as a control. The proliferation rate of the cells compared with the control was then assessed using the Sulforhodamine-B proliferation assay. Cells exposed to blue light showed a statistically significant (P<0.05) increase in proliferation. Those exposed to blue light through a standard ultraviolet absorbing intraocular lens showed a smaller increase in proliferation, whereas those exposed with a blue light filtering intraocular lens showed no increase in proliferation than the control in all four cell lines. The exposure of cells to blue light led to an increase in proliferation in all cell lines compared with the control. The use of blue light filtering intraocular lenses abolished these increases in proliferation in the four cell lines. These results indicate that blue light filtering intraocular lenses may have a protective effect on the proliferation rates of uveal melanoma cells exposed to blue light. © 2006 Lippincott Williams & Wilkins, Inc.