ORIGINAL ARTICLEExpression of integrins, degradative enzymes and their inhibitors in uveal melanoma: differences between in vitro and in vivo expressionBaker, J. K. L.; Elshaw, S. R.; Mathewman, G. E. L.; Nichols, C. E.; Murray, A. K.; Parsons, M. A.; Rennie, I. G.; Sisley, K.*Author Information Institute for Cancer Studies, University of Sheffield, Medical School, Beech Hill Road, Sheffield, S10 2RX, UK (J. K. L. Baker, A. K. Murray). Department of Ophthalmology and Orthoptics, O Floor, University of Sheffield, Royal Hallamshire Hospital, Glossop Road, Sheffield, S10 2JF, UK. Tel: (+44) 114 2713199; Fax: (+44) 114 276 6381; Email: [email protected] (S. R. Elshaw, G. E. L. Mathewman, C. E. Nichols, M. A. Parsons, I. G. Rennie, K. Sisley). (Received 1 October 2000; accepted in revised form 27 December 2000) *To whom correspondence should be addressed Melanoma Research: June 2001 - Volume 11 - Issue 3 - p 265-273 Buy Abstract Posterior uveal melanoma is the most common intraocular malignancy in adults. Metastasis occurs in approximately 40% of all cases and spread is primarily to the liver. Once secondary hepatic disease has developed the prognosis is poor. Metastasis involves a series of adhesion and de-adhesion events, coupled with regulated tissue degradation to facilitate tumour cell invasion and spread to both local and distant sites. These processes are assisted by the expression of integrins and degradative enzymes by both tumour and host cells. Using a series of 10 uveal melanomas, we investigated the expression of a panel of integrins, degradative enzymes and their inhibitors that have been shown to be associated with metastasis. In addition, we undertook to establish if there might be differential expression in response to growth under artificial conditions. All the tumours expressed matrix metalloproteinases (MMP)-2 and-9, tissue inhibitor of metalloproteases (TIMP)-2, urokinase plasminogen activator (u-PA), plasminogen activator inhibitor (PAI)-1 and PAI-2. Differences in the expression of the integrins α1β1, α2β1 and α6β1 were observed; in particular, these differences appeared to relate to expression as a consequence of growth in culture. In summary, uveal melanoma cells express both degradative enzymes and their respective inhibitors, which are important in metastasis. It would appear that differential expression of integrins is present, probably as a response to in vitro stimulation. © 2001 Lippincott Williams & Wilkins, Inc.