Original ArticlesEstablishment of IPC 227 cells as human xenografts in rabbits: a model of uveal melanomaBonicel, P.*; Michelot, J.; Bacin, F.; Papon, J.; Kemeny, J. L.; Moins, N.; Morvan, D.; Madelmont, J. C.Author Information CHU Gabriel Montpied, Service d'Ophtalmologie, 63000 Clermont-Ferrand Cedex, France. Tel: (+33) 473625716; Email: [email protected] (P. Bonicel, F. Bacin). INSERM U484, Clermont-Ferrand Cedex (J. Michelot, J. Papon, N. Moins, D. Morvan, J. C. Madelmont). CHU Gabriel Montpied, Laboratoire d'Anatomo-pathologie, Clermont-Ferrand Cedex (J. L. Kemeny). *To whom correspondence should be addressed Received 9 February 2000; accepted in revised form 11 May 2000 Melanoma Research: October 2000 - Volume 10 - Issue 5 - p 445-450 Buy Abstract This study was designed in order to evaluate the feasibility of establishing an animal model of human uveal melanoma. IPC227, a cell line established from the biopsy of a patient with a spindle cell ciliary body melanoma, was transplanted into the anterior chamber of the eye in immunosuppressed New Zealand rabbits. In a second step, a tumour fragment from the anterior chamber was implanted transclerally into the posterior choroid. Complete ophthalmological examinations were then performed on the animals. Characteristic growth patterns were noted depending on the location of implantation. In the anterior chamber, diffuse, flat, heavily pigmented tumours appeared 8 days after the injection of the cell suspension that covered the iris and the angle by day 25, with a success rate of 100%. Nodular, lightly pigmented tumours were obtained 6–7 weeks after subchoroidal implantation, with a 25% success rate. Clinical examination, including fundus photography, ultrasound and magnetic resonance imaging, demonstrated the same characteristics as those of human uveal melanoma, confirming the value of this model for the evaluation of new therapeutic and diagnostic methods in the management of uveal melanoma. © 2000 Lippincott Williams & Wilkins, Inc.