ORIGINAL ARTICLE: PDF OnlyExpression of interleukin-8 in primary and metastatic malignant melanoma of the skinSingh, R. K.; Varney, M. L.; Bucana, C. D.; Johansson, S. L.Author Information Department of Pathology and Microbiology, University of Nebraska Medical Center, 985660 Nebraska Medical Center, Omaha, NE 68198–5660, USA. Melanoma Research: August 1999 - Volume 9 - Issue 4 - p 383-388 Buy Abstract It was recently demonstrated that interleukin-8 (IL-8) is produced by cultured melanoma cells and acts as an essential autocrine growth factor. Earlier studies from our laboratory demonstrated a direct correlation between IL-8 expression in human variant cell lines and their metastatic potential in nude mice. In the present study we examined the expression of IL-8 in human malignant melanomas using immunohistochemistry to correlate IL-8 levels with disease stage. None of the radial growth phase (RGP) tumours (melanoma in situ) expressed IL-8. In contrast, 50% of the vertical growth phase (VGP) tumours (invasive melanoma), which have a high risk of metastasis, showed IL-8 immunoreactivity. Further, all the metastatic lesions analysed showed intense staining for IL-8; the levels were higher than those observed in the primary skin tumours. In summary, the data suggest an association between the expression of IL-8 and the metastatic phenotype. © 1999 Lippincott Williams & Wilkins, Inc.