ORIGINAL ARTICLE: PDF OnlyA synthetic peptide inhibitor for α-chemokines inhibits the tumour growth and pulmonary metastasis of human melanoma cells in nude miceFujisawa, N.; Hayashi, S.; Miller, E. J.Author Information Department of Biochemistry, The University of Texas Health Center at Tyler, 11937 US Hwy. 271, Tyler, TX 75708–3154, USA. Melanoma Research: April 1999 - Volume 9 - Issue 2 - p 105-114 Buy Abstract Growth-related oncogene-α (GROα) was first described as an autocrine mitogen and growth factor for melanoma cells. More recent studies show that GROα, interleukin-8 (IL-8) and other members of the α-chemokine superfamlly are also angiogenic. Therefore, we sought to determine if inhibitors of the α-chemokine receptor would be effective in inhibiting the tumour growth and pulmonary metastasis of human melanoma cells. We determined that melanocytes and 12 human melanoma cell lines produce both GROα and IL-8. The proliferation of A375SM, a highly metastatic cell line, and C8161-C were significantly increased by human recombinant GROα and inhibited by anti-human GROa monoclonal antibody. Antileukinate, a potent inhibitor of α-chemokine receptor binding, inhibited the binding of GROα to its receptors in melanocytes and all 12 melanoma cell lines tested. Antileukinate also suppressed proliferation of A375SM and C8161-C cells in a dose-dependent manner, and the suppression was not due to cytotoxic effects. Furthermore, continuous administration of antileukinate inhibited the tumour growth and pulmonary metastasis of A375SM cells in athymic BALB/c nude mice. These findings suggest that antileukinate inhibits the growth of melanoma cells by preventing GROα from binding to its receptors. This suggests a possible use of α-chemokine receptor inhibitors such as antileukinate in the treatment of malignant melanoma. © 1999 Lippincott Williams & Wilkins, Inc.