Original Articles: PDF OnlySelective growth of human melanoma cells in the brain parenchyma of nude miceFujimaki, T1,3; Price, J E1; Fan, D1; Bucana, C D1; Itoh, K2; Kirino, T3; Fidler, I J1Author Information 1Department of Cell Biology, Box 173, Fax: ( + 1) 713 792 8747 2Department of Immunology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA. 3Department of Neurosurgery, The University of Tokyo, Tokyo, 113 Japan Melanoma Research: October 1996 - Volume 6 - Issue 5 - p 363-371 Buy Abstract The purpose of this study was to determine whether the growth of human melanoma cells in the brain parenchyma Is selective and represents the growth of unique celts. Six human melanoma cell lines derived from cutaneous lymph node or brain metastases (from six different patients) and melanoma cells isolated from fresh surgical specimens of two primary cutaneous melanomas, two lymph node metastases and two brain metastases (each from a different patient) were injected into the subarachnold space of nude mice. All melanomas produced growths in the leptomeninges, but only melanoma cells Isolated from brain metastases infiltrated into and grew in the brain parenchyma of nude mice. The results from in vitro assays for cell motility or production of gelatlnase activity did not correlate with In vivo growth pattern. However, the In vitro growth of human melanoma cells in the presence of TGF-β2 inversely correlated with potential for brain parenchyma metastasis, I.e. the growth of cells from brain metastases was least inhibited by TGF-β2- These data suggest that melanoma brain parenchyma metastases are produced by unique cells that may be resistant to the antiproliferative effects of TGF-β.2 © Lippincott-Raven Publishers.