Original Articles: PDF OnlyMutation and expression of the low affinity neurotrophin receptor in human malignant melanomaPapandreou, C1; Bogenrieder, T1; Loganzo, F1; Chao, M V2; Nanus, D M1,3; Albino, A P1Author Information 1Laboratory of Mammalian Cell Transformation, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA. Fax: (+1) 212 794 4352 2Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY, USA 3Genitourinary Section, Division of Solid Tumor Oncology, Department of Medicine, Memorial Sloan-Kettering Cancer Center and Cornell University Medical College, New York, NY, USA Melanoma Research: October 1996 - Volume 6 - Issue 5 - p 373-378 Buy Abstract The low affinity p75 neurotrophin receptor (p75NTR) is a cysteine-rich transmembrane glycoprotein which is frequently overexpressed in advanced stages of human melanoma. The biological consequences of this overexpression are unknown; however, It has recently been shown that p75NTR can enhance the invasive potential of melanoma cells in vitro. In the present study we examined cell lines established from normal human melanocytes and metastatic melanomas for expression of p75NTR mRNA and protein. The results showed that, compared with normal melanocytes, levels of p75NTR-specific protein were high in seven melanoma lines, markedly decreased in two melanoma lines and comparable in two melanoma lines. The conserved transmembrane domain of p75NTR was analysed for point mutations by single strand conformation polymorphism analysis and direct DNA sequencing. Identical point mutations were detected in the transmembrane domain of p75NTR in the two melanoma lines with reduced p75NTR protein expression, which resulted in the substitution of the uncharged amino acid Gly for the negatively-charged Asp. © Lippincott-Raven Publishers.