Given the fact that Bowen's disease is considered a high grade dysplasia (AIN3) with a low risk of invasion (around 5%), we adopted a conservative approach undergoing a complete excision of the lesion and, once achieved, proposing to the patient a course of supplementation with a multistrain probiotic with daily endorectal instillation of 900 billion live bacteria and daily oral administration of 3 sachets, each containing 450 billion live bacteria (probiotic composition: Lactobacillus plantarum DSM 24730, Streptococcus thermophilus DSM 24731, Bifidobacterium breve DSM 24732, Lactobacillus paracasei DSM 24733, Lactobacillus delbrueckii subsp, bulgaricus DSM 24734, Lactobacillus acidophilus DSM 24735, Bifidobacterium longum DSM 24736, and Bifidobacterium infantis DSM 24737; commercially available as Vivomixx in Europe, Visbiome in USA and DeSimone Formulation in Korea), with the aim to reduce local inflammation and to rebalance local microbiota, thus possibly restoring in site mucosal immunity.
After 4 months of supplementation, a new pelvic MRI scan evidenced the spot of the previous biopsy but no evidence of mesorectal, iliac, and pelvic lymphadenopathies. Moreover, clearance of the anal condylomas previously described was noted (Fig. 2B) despite HPV18 DNA was still detectable.
Six months later, the patient underwent a new HRA that showed no evidence of condyloma.
This case report suggests a potential role for this specific probiotic formulation in promoting the regression of anal HPV related lesions in HIV-infected individuals. The effects of a number of oral probiotic formulation on HPV associated alterations have been mainly studied in the female genital tract; in a prospective controlled pilot study, women with HPV associated low-grade squamous intraepithelial lesions were randomized for probiotic supplementation: the treated group showed a higher chance of clearing HPV infection (29% vs 19%, P = .41) and higher rates of clearance of HPV associated cytological abnormalities (60% vs 31%; P = .05) in comparison to the control group. Other evidences show that a reduced amount of Lactobacillus spp. combined with an increased diversity of the vaginal microbiota could be involved in HPV acquisition and persistence and in the development of dysplastic cervical lesions[12,13]; moreover, in vitro studies demonstrated a direct cytotoxic effect of certain vaginal microbial strains on HPV-infected cells and an inhibition of the expression of HPV E6 and E7 oncogenes, which may prevent malignant transformation in individuals harboring high-risk HPV genotypes.[14–16]
Based on the findings of the mentioned gynecological studies, in which the prescription of probiotic supplementation seems related with the regression of HPV related female genital tract lesions, its our opinion that probiotics could also exerted a therapeutic role in our patient. In fact, despite at the moment there are no similar direct evidences for anal condylomatosis, we believe that the effects of probiotic strains (described for female genital tract) are also possible in the anal district, as observed in this case.
Moreover in HIV positive patients, dysbiosis of the gut microbiota largely contributes to the impairment of mucosal immunity and could facilitate HPV persistence and the subsequent epithelial transformation. Given that spontaneous regression of multiple large condylomas, such as those observed in our patient, is not frequently seen among HIV-infected patients, we hypothesize a beneficial role exerted by this specific probiotic formulation in the clearance of HPV related lesions.
Finally, data on the effects of probiotic rectal enema on anal HPV infection in HIV positive males are lacking. Given the extremely distal localization of the HPV related lesions of our patient, we decided to use, together with oral administration, rectal enemas of probiotics to maximize the amount of bacterial cells that would reach the anal canal to effectively interact with the local epithelium.
Growing evidences show the potential beneficial effect of probiotics on HPV related disease, also in anal district. In particular, in HIV-infected individuals, due to the impairment of the immune response and the loss of gut intraepithelial lymphocytes, lower rates of HPV clearance are observed and consequently spontaneous resolution of multiple large anal condylomas is rare among this population. For this reason, the positive results of our preliminary experience support the hypothesis of a direct efficacy of oral and rectal multistrain probiotic administration in the treatment of anal condylomatosis in HIV-infected patients.
Conceptualization: Giancarlo Ceccarelli, Gabriella d’Ettorre.
Data curation: Giancarlo Ceccarelli, Stefano Savinelli, Luigi Bianchi, Alessandra Pierangeli, Francesco Vullo, Antonio Ciardi, Gabriella d’Ettorre.
Formal analysis: Francesco Vullo.
Investigation: Stefano Savinelli, Luigi Bianchi, Alessandra Pierangeli, Francesco Vullo.
Methodology: Giancarlo Ceccarelli, Alessandra Pierangeli.
Resources: Stefano Savinelli, Alessandra Pierangeli.
Supervision: Antonio Ciardi, Gabriella d’Ettorre.
Validation: Alessandra Pierangeli, Gabriella d’Ettorre.
Visualization: Luigi Bianchi.
Writing – original draft: Giancarlo Ceccarelli, Gabriella d’Ettorre.
Writing – review & editing: Giancarlo Ceccarelli, Eugenio Nelson Cavallari, Antonio Ciardi.
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Keywords:Copyright © 2018 The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved.
Bowen's disease; condylomas; HIV; HPV; papilloma; probiotics; squamocellular intraepithelial carcinoma