Breast cancer is the most common cancer in women after non-melanoma skin cancer, and it is the leading cause of cancer-related deaths in Croatia. About 5% to 10% of all breast cancer cases are hereditary, and heterozygous germline BRCA1 and BRCA2 mutations are responsible for the majority of hereditary breast and/or ovarian cancers. In the most cases, the mutations are small nucleotide alterations leading to premature stop of translation. Large rearrangements of BRCA1 and less often, BRCA2 have been described in recent years.
In the Laboratory for Hereditary Cancer at the Rudjer Boskovic Institute about 240 patients have been analyzed for both small mutations and large rearrangements of BRCA genes and this is the first large deletion found.
2 Case presentation
The patient, a 36-year-old woman, developed Her-2 positive breast cancer at the age of 29 in her left breast, and triple negative breast cancer at the age of 33 in her right breast. The initial interview indicated that there was no history of cancer in the family, but testing for BRCA1 gene mutation was indicated due to the early age of onset and her triple negative status (Table 1). The patient signed her informed consent for BRCA testing and the study was conducted according to the Declaration of Helsinki Principles. The mutation analysis was conducted with HRM (high-resolution melting) for small mutations and QMPSF (quantitative multiplex polymerase chain reaction of short fluorescent) for larger rearrangements. Deletion of exons 4 to 6 (traditionally named exons 5–7) in BRCA1 was found with QMPSF and confirmed with MLPA (multiplex ligation-dependent probe amplification; name of the kit used for the experiment ‘SALSA MLPA P002 BRCA1 probemix; MRC-Holland’).
After the mutation was found, the consulting physician conducted an additional interview with following family history: There was no cancer history in maternal side (and testing the patients’ mother and sister did not show the mutation), but on the fathers side there was a history of cancer. The father died from heart attack at the age of 47 and did not have cancer at the time of death. The patient's paternal grandfather died from lymphoma at the age of 64. Two grandfather's sisters developed uterine cancer and one of them died from it at the age of 43. Two grandfather's sisters developed colon cancer. Two daughters from one of the grandfather's sisters died from cancer, one had lung cancer and the other uterine cancer. No family member from the father's side was available for testing.
To determine the deletion breakpoints, several pairs of primers were developed to bridge the gap. Long range PCR using the Expand Long Range dNTPack (Roche) as recommended by the manufacturer was performed. The PCR products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. The bands were purified with QIAquick Gel Extraction Kit (QIAGEN) and sequenced on ABI PRISM 310 Genetic Analyzer (Applied Biosystems).
Long-range PCR resulted in the fragment of 356 bp. Sequencing determined a 10,257 bp deletion with HGVS nomenclature NC_000017.11:g.43100079_43110335del10257, NG_005905.2:g.107648_117905del, NM_007294.3:c.135–3803_442–199del, NP_009225.1:p.(Lys45Asnfs*16) (Fig. 1).
De novo BRCA1 mutations are very rare, and have been reported only few times. Since it is unlikely that the same breakpoints would occur independently, if this mutation had the same breakpoints as previously reported, it is not new. The literature survey showed this deletion (almost always named exon 5–7 deletion) to be reported 6 times previously. In 3 cases the deletion size was not determined (Italy, Slovenia, and Myriad Genetic Laboratories); in 2 cases the deletion was 4995 bp (Spain and Denmark), and in 1 case it was 5024 bp (Germany).
This deletion did not correspond to the mutations found in Germany and Denmark/Spain, which were much smaller (Fig. 2), so the authors from Italy and Slovenia were contacted. The Slovenian DNA was not available, but the mutation from Italy is not the same, since the primers for the long-range PCR gave no product.
Large rearrangements in BRCA genes are not a common occurrence in Croatian population; this is the first one in more than 10 years of testing. Identification of this large deletion of BRCA1 gene presents a valuable result that shows the need of doing the large rearrangement analysis even in populations where it is not usually seen.
It also demonstrates the problem of lack of the complete family history in determination if the mutation is de novo. It would be helpful if all large rearrangements were described at the nucleotide level, so the origin of these mutations can be more easily resolved.
We kindly thank Dr Alessandra Viel for the use of Italian DNA sample for comparison.
. Judkins T, Rosenthal E, Arnell C, et al. Clinical significance of large rearrangements in BRCA1
and BRCA2. Cancer 2012;118:5210–6.
. Levanat S, Musani V, Cvok ML, et al. Three novel BRCA1
/BRCA2 mutations in breast/ovarian cancer families in Croatia. Gene 2012;498:169–76.
. Golmard L, Delnatte C, Laugé A, et al. Breast and ovarian cancer predisposition due to de novo BRCA1
and BRCA2 mutations. Oncogene 2016;35:1324–7.
. Agata S, Viel A, Della Puppa L, et al. Prevalence of BRCA1
genomic rearrangements in a large cohort of Italian breast and breast/ovarian cancer families without detectable BRCA1
and BRCA2 point mutations. Genes Chromosomes Cancer 2006;45:791–7.
. Novaković S, Milatović M, Cerkovnik P, et al. Novel BRCA1
and BRCA2 pathogenic mutations in Slovene hereditary breast and ovarian cancer families. Int J Oncol 2012;41:1619–27.
. Palanca Suela S, Esteban Cardeñosa E, Barragán González E, et al. Identification of a novel BRCA1
large genomic rearrangement in a Spanish breast/ovarian cancer family. Breast Cancer
Res Treat 2008;112:63–7.
. Hansen Tv, Jønson L, Albrechtsen A, et al. Large BRCA1
and BRCA2 genomic rearrangements in Danish high risk breast-ovarian cancer families. Breast Cancer
Res Treat 2009;115:315–23.
. Preisler-Adams S, Schönbuchner I, Fiebig B, et al. Gross rearrangements in BRCA1
but not BRCA2 play a notable role in predisposition to breast and ovarian cancer in high-risk families of German origin. Cancer Genet Cytogenet 2006;168:44–9.