The early survival rate of preterm infants, in recent 30 years, has been greatly increased as the development of assisted reproductive technologies.1–3 However, immature oral feeding ability has severely negatively impact on the normal development of preterm infants and even obviously increased the morbidity in this given population.4 Coordination of sucking–swallowing–breathing (SSB) movements, which usually tend to mature until 32 to 34 gestational weeks, is an essential to develop the delicate oral feeding in infants.5 The full-term infants can successfully complete the SSB activity, but preterm infants cannot.
Published studies revealed that nonnutritive sucking (NNS), which is closely associated with gestational age (GA), may improve the efficacy of oral feeding in preterm infants.6–9 However, the preterm infants characterized by immature cardiorespiratory system, central venous system, and oral musculature will suffer from some threatening clinical outcomes which included bradycardia, apnea, and low oxygen saturation when changed feeding approach from tube to totally oral feeding,10–12 and this condition was defined as oral feeding difficulty which is associated with the longer length of hospital stays (LOS), more medical costs, and serious psychological stress of parents after parturition, as well as even caused long-term oral feeding difficulties both related to bottle and breast feeding.13,14
Some studies published previously suggested that early oral motor intervention (OMI), which consists of oral stimulation, oral support, and NNS, can better the effects of oral feeding in preterm infants and shorten the LOS.15–17 However, the powers of conclusions were impaired due to some shortages such as small sample size existed in across studies. Although a systematic review was performed by Arvedson et al18 to determine whether the OMI can improve the oral feeding ability of preterm infants, only studies published between 1960 and 2007 and in English, however, met its criteria. The Cochrane Collaboration recommended that a systematic review should be updated every other year,19 and it is important that a plenty of randomized controlled trials (RCTs) have been developed since then. Hence, we aimed to further evaluate the potential of OMI for oral feeding in preterm infants by undertook this meta-analysis with trial sequential analysis (TSA).
MATERIALS AND METHODS
We performed our meta-analysis according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statement20 and Cochrane Handbook for Systematic Reviews of Interventions.21 The prospective protocol of this topic has been registered on PROSPERO database and the register number of CRD42014014356 has been approved (available at: http://www.crd.york.ac.uk/prospero/). Ethical approval and informed consent were not required because all analyses were carried out based on these data extracted from published previously studies and no clinical prejudice was put on patients.
A computerized search of PubMed, EMBASE, Web of Science, the Cochrane Library, and China National Knowledge Infrastructure (CNKI) was performed by using medical subject heading and full-text words based on Boolean logic operator. The flowing searched terms were used: “Infant, Premature,” “Infants, premature,” “Premature Infant,” “Preterm Infant,” “Infant, Preterm,” “Infants, Preterm,” “Neonatal Prematurity,” “oral motor intervention,” “oral motor exercise,” “oral stimulation,” OMI, and random*. The lists of references of included articles and reviews, which were related to our topic, were manually searched to capture any relevant studies. We summarized the search strategy of PubMed in Appendix 1, https://links.lww.com/MD/A362.
An appropriate selection criterion is the key factor to guarantee the accurate studies identification. So we established this inclusion criteria according to the PICOS acronym: P (Population): all the patients diagnosed as preterm infants; I (Interventions) and C (comparisons): OMI compared with route interventions only; O (Outcomes): the transition time, LOS, feeding efficiency, intake of milk, and weight gain were assessed in our meta-analysis; S (Study Design): only RCTs with appropriate random sequence generation met the criteria. In addition, for duplicate data reported by the same author or 1 medical center, the article with high quality was included. Meanwhile, we included studies published in English and Chinese language.
Literature Screened and Data Extraction
Search was conducted, and data were extracted by 2 independent investigators (XT and L-JY). Each trial captured in the search stage was evaluated for author, publication year, the number of participants, allocation method, and patients’ age, and interventions, period of treatments, eligibility criteria, baseline, and outcome measures of interest. Any divergences concerning the eligibility of a trial occurred in the any phases were resolved through discussion or consulting a third investigator (LZ).
Assessment of Risk of Bias
Two independent investigators (J-GZ and LM) assessed the methodological quality of trials included in our meta-analysis by using the Cochrane Risk of Bias Tool.21 The procedure was performed based on the following 7 domains: randomization sequence generation, allocation concealment, blinding of participants and study personnel, blinding of outcome assessors, incomplete outcome data, selective reporting, and other biases. Based on the information extracted from each eligible trial, each domain was rated as “high risk,” “unclear risk,” or “low risk.” These domains will be classified as high risk unless appropriate methods were used; in contrast, corresponding domains will be graded as low risk when no obvious mistakes were detected; moreover, associated domains will be rated as unclear risk if lack of sufficient information to make a clear judgment on the risk of bias. Agreement on any domain was identified based on consensus or consulting a third investigator (Y-XO).
All extracted data were entered into RevMan 5.3 (Copenhagen: The Nordic Cochrane Centre, The Cochrane Collaboration, 2013) for statistical analysis. Mean difference (MD) or standard mean differences (SMD) with 95% confidence interval (CI) for continuous outcomes were selected to estimate the pooled effect size. Heterogeneity in the included studies was evaluated using the χ2, corresponding P value and I2 statistic. If I2 ≥ 50%, the eligible studies were considered to be heterogeneity and a random-effects model based on Mantel–Haenszel (MH) or inverse variance (IV) statistical approach was selected. In contrast, the studies were considered to be homogeneous, and a fixed-effects model based on MH or IV statistical approach was selected. Changed data were selected to calculate the summary results according to the baseline and endpoint data.22
Repeated significance test of sparse and accumulated data has a risk to yield random errors which cause false positive or negative results.23–25 For single primary trial, sequential analysis based on group sequential is similar to interim analysis that may increase the risk of type I errors. So the monitoring boundaries were developed and applied to determine if the trial should be ended early under the condition of a diminutive P value, which indicates statistical significant difference between study groups to minimize the type I error.26 It is possible that sequential analysis, which can also be titled with TSA, can be adopted to analyze the pooled results of meta-analysis.23 The quantification of the required information size (RIS) is a major factor to realize the TSA. We calculated the RIS adjusted for diversity because the heterogeneity adjustment with I2 will underestimate the RIS value.19 The TSA was performed at the level of an overall 5% risk of a type I error and 20% of the type II error (a statistical test power of 80%).27 If the Z-curve across the monitoring boundary, then we can draw the conclusion of getting credible conclusion before surpassing the RIS line. If the Z-curve across the futility boundary, then we can come to the conclusion of this intervention have no effect for this outcome even though the RIS was not reached. The reliable conclusion can be drawn if the adjusted monitory boundary was surpassed and/or RIS was reached. Because effect measures selected in this meta-analysis fall into continuous data category, and the outcomes of transition time, LOS, feeding efficiency, intake of milk, and weight gain, we estimated the RIS based on the empirical data autogenerated from software according to the data input.28 TSA software (version 0.9 beta) was available at http://www.ctu.dk/tsa/.
Study Selection and Basic Characteristics
A total of 63 citations were captured at the initial literature search stage and add no trail to the searched result. Finally, 11 trials,16,29–38 which included 855 participants, were remained according to inclusion and exclusion criteria. The flow diagram of literature retrieval and selection was shown in Figure 1.
Assessment of Risk of Bias
A total of 855 participants were included into our meta-analysis. Basic characteristics of all eligible studies are shown in Table 1. Most of them have problems about blinding. Low-level literatures were resulted from incomplete outcome data. Although reported the dropouts, these studies have no intention-to-treat (ITT) analyses. The methodological quality assessments of included trials were shown in Figure 2.
Meta-Analysis on Transition Time
Ten of all trials involving 780 participants reported the transition time. Heterogeneity was identified across the included studies (P = 0.01; I2 = 57%). Hence, a random-effects model, which indicates all the participants from included trials were sampled from the different population, was selected to summarize mean effect size because the resources caused heterogeneity were not detected among included trials. Meta-analysis suggested that OMI can effectively shorten the transition time, with statistical significant difference (MD, −4.03; 95% CI, −5.22 to −2.84) (Figure 3). Sensitivity analysis based on different pooled model was adopted to test the robustness of pooled result. Pooled result from fixed-effects model was the same with that of random-effects model, and well suggested that the summary effect size is robust (MD, −4.03; 95% CI, −5.22 to −2.84). We undertook a TSA at the level of α of 0.05, β of 0.2, and then an RIS of 279 was calculated. This pooled result was considered to have reliability resulted from Z-curve across conventional statistically significance test boundary, TSA-adjusted boundary value, and the cumulative number of patients reached RIS of 279 (Figure 4). So, OMI has the potential for transition time on preterm infants and worth clinical use.
Meta-Analysis on LOS
Six trials, which include 436 participants, were enrolled in our meta-analysis calculating the LOS. We identified homogeneity in the 6 studies assessed (P = 0.27, I2 = 21%). Therefore, a fixed-effects model, which indicates all the participants from included trials were sampled from the same population, was performed to calculate mean effect size. Meta-analysis revealed that OMI effectively shortened the LOS, with statistical difference (MD, −3.64; 95% CI, −5.57 to −1.71) (Figure 5). TSA was taken in the condition of α of 0.05, β of 0.2, and figured out RIS of 851. Although the accrued number of patients did not reach RIS of 851, the cumulative Z-curve cross conventional significance test boundary, RIS-adjusted boundary value, and the effect was prior established (Figure 6). So, OMI has effect for the LOS on preterm infants and worth clinical use.
Meta-Analysis on Feeding Efficiency
Three trials, which included332 preterm infants, were enrolled in the meta-analysis identifying the feeding efficiency. There was homogeneity about the 3 studies (P = 0.78, I2 = 0%). Therefore, a fixed-effects model of analysis was used. Meta-analysis result showed OMI can greatly improve the feeding efficiency, with statistical significant difference (MD, 0.81; 95% CI, 0.36–1.27) (Figure 7). TSA was performed in the level of α of 0.05, β of 0.2, and demonstrated RIS of 430. Even though the cumulated number of patients did not reach the value of RIS, the cumulative Z-curve cross conventional statistically significant boundary, TSA-adjusted boundary value and confirmed the result of reliability early (Figure 8). So, OMI has the potential for feeding efficiency on premature infants and worth clinical use.
Meta-Analysis on Intake of Milk
Three trials including 332 premature infants reported the intake of milk. Homogeneity was detected in the incorporated studies (P = 0.25, I2 = 27%), and then a fixed-effects model of analysis was performed to calculate mean effect size. OMI can validly enhance the intake of milk, with statistical difference (MD, 0.14; 95% CI, 0.06–0.21) (Figure 9). TSA was performed, and RIS of 430 was counted in the condition of α of 0.05, β of 0.2. The pooled result manifested reliability due to Z-curve across conventional statistically significant boundary, TSA-adjusted boundary although the RIS was not accrued (Figure 10). So, OMI has efficiency for intake of milk on premature infants and worth clinical use.
Meta-Analysis on Weight Gain
Three of all eligible trials, involving 318 patients, were enrolled in the meta-analysis reporting the weight gain. Heterogeneity was checked in eligible studies (P = 0.00, I2 = 88%). We chose a random-effects model to summarize mean effect size. Meta-analysis result revealed that OMI cannot validly increase the weight gain (MD, −17.54; 95% CI, −151.34–116.26) (Figure 11). RIS and TSA adjusted boundary value cannot be calculated due to limited information size. So whether OMI was effective for weight gain on preterm infants, it still needs more researches to establish.
We performed a funnel plot to examine the publication bias in all of the included studies. The outcome from the funnel plot analysis is shown in Figure 12, which shows symmetry, thereby indicating that no publication bias possibly exists in the included trials.
Advances in reproductive technologies brought out the significant improvements for survival rate of preterm infants in recent years.39 The underdevelopment of cardiopulmonary system, central nervous system, and oral muscle tissue is responsible for lack of the ability of coordination of SSB for preterm infants, which often results in oral feeding difficulties and oral feeding ineffectiveness,40–44 prolonged LOS, and increased burden of family and eventually the whole society.45 Achievement of oral feeding is a primary criterion for discharge for healthy preterm infants.46 Hence, successfully and effectively transited feeding approach from tube to oral feeding is the important target for health care staffs.
To generate reasonable and reliable pooled results, we selected the Cochrane risk of bias tool to critically assess the methodological quality. As the domain of incomplete outcome data, 4 included studies were judged with high risk of bias. Although reported the dropouts, intention-to-treat (ITT) was not undertook. So, we cannot judge whether the dropouts may impair the pooled results. In blinding of outcome assessment, one research was evaluated high risk of bias, which may result from inappropriate design for blinding, and 7 were unclear risk of bias, obvious information about blinding of outcome assessment cannot be identified in these researches, and may lead to evaluation bias. For blinding of participants and personnel, 7 studies were termed as unclear risk of bias, and specific explanations for this domain were not found. Impertinent blinding may lead to results away from true value and produce measurement bias. Implementing blinding for participants or not will not negatively or positively affect the process of whole study due to the target population included in our study is premature infant. Owing to all outcomes were objective, it cannot influence the outcomes in a large extent for whether implement blinding for personnel. For the domain of allocation blinding, 6 researches were judged unclear risk of bias. Insufficient allocation concealment may cause overestimating effect of intervention. Hence, we should evaluate the reliability of pooled results with prudence. In order to draw a more reliable conclusion, we hope that researchers could emphasize on blinding, allocation concealment, and complete outcome data in further studies. Among 11 studies included in this research, only 1 study was eligible for all domains of quality of methodology. Low-quality researches have greater bias in quality control and will affect the results of this study to some extent.
The meta-analysis results revealed that OMI can effectively improve the condition of transition time, LOS, feeding efficiency, and intake of milk related to route care. But no difference was identified in weight gain between both groups. We summarized the evidence to promote clinical use and further research on this topic in Table 2. The success exploration of OMI and its operation process reflect the limitations of traditional care for preterm infants. We hope that clinical staffs can improve and update their old care manner and make use of the direction of evidence-based nursing theory. Published evidences suggested that early OMI has effects on oral feeding in preterm infants and can shorten the LOS. However, the conclusion is still controversial with some drawbacks, such as small sample size, which can lead to low power regarding the effects of OMI for premature infants. Lack of power may result to get false-negative results, whereas this work combined with TSA to test whether this pooled results were robust and have credibility. And owing to time goes by, techniques of this intervention also improve; so it is needed to explore whether it is effective after about decade years. In addition, this systematic review included studies published between 1960 and 2007, whereas only English literature is eligible for its inclusion criteria11 and the outcomes may be impaired by selection bias. So it is imperative to do this meta-analysis.
Our meta-analysis has only searched the PubMed, the Web of Science, EMBASE, the Cochrane Library and CNKI, but not SpringerLink, ScienceDirect, Chinese Biomedical Literature Database, and other relevant electronic database and unpublished sources, so there is a risk of incomplete retrieval. In addition, this study included only literature published in English and Chinese language, and the language restriction may result in selection bias, which affects the credibility of the pooled results of our meta-analysis.
OMI is beneficial for oral feeding in preterm infants, but it still has some questions for clinical promotion. For example, the positive function of NNS has formed a consensus, but the application of the time and intensity of NNS not yet formed unified regulations. OMI for preterm infants should be more standardized and systematic. So, more studies need to do to further explore a standard operational approach. In recent years, due to a different mechanism of NNS and oral stimulation, foreign scholars began to explore the influence of the combined use of oral feeding and NNS in premature infants, as well as to determine whether the combination intervention regime is superior to one of all alternatives alone. Some of outcomes for included results were significant. However, they cannot use the pooled analysis for lacking of enough information. But these outcomes have significant value for assessing sucking ability and worth to use in future studies. So, we hope researchers do more studies to provide more standardized, scientific, rationalized approach for clinical use. Although the included studies have differences in the definition of GA for preterm infants, but the sensitivity analyses showed heterogeneity and have little effect on the pooled results. This study uses TSA to calculate the RIS of α of 0.05 and β of 0.2, and it showed credibility for these pooled results.
In conclusion, OMI can effectively improve the condition of transition time, LOS, feeding efficiency, and intake of milk, so it is worthy to be used widely in hospitals to improve the clinical outcomes of preterm infants. While RCTs with large-scale and high-quality based on RIS are warranted to further investigate the effectiveness of OMI for weight gain and may explore whether it has the potential for other variable on preterm infants such as later growth and development.
These authors would like to thank editor and anonymous referees for their valuable and informative comments. Moreover, the authors also thank Professor Yan Wang for her assistance in this work. Most importantly, on behalf of all coauthors, the first author would like to thank the editorial office of Chinese Journal of Nursing published in Chinese language reported part of results generated from the same topic.
1. Jadcherla SR, Shaker R. Esophageal and upper esophageal sphincter motor function in babies. Am J Med
2001; 111 (Suppl 8A):64S–68S.
2. Kelly MM. The medically complex premature infant in primary care. J Pediatr Health Care
3. Kramer MS, Demissie K, Yang H, et al. The contribution of mild and moderate preterm birth to infant mortality. Fetal and Infant Health Study Group of the Canadian Perinatal Surveillance System. JAMA
4. Howe TH, Sheu CF, Hinojosa J, et al. Multiple factors related to bottle-feeding performance in preterm infants. Nurs Res
5. Kish MZ. Oral feeding readiness in preterm infants: a concept analysis. Adv Neonatal Care
6. Kish MZ. Improving preterm infant outcomes: implementing an evidence-based oral feeding advancement protocol in the neonatal intensive care unit. Adv Neonatal Care
7. Miller JL, Kang SM. Preliminary ultrasound observation of lingual movement patterns during nutritive versus non-nutritive sucking in a premature infant. Dysphagia
8. South MMT, Strauss RA, South AP, et al. The use of non-nutritive sucking to decrease the physiologic pain response during neonatal circumcision: a randomized controlled trial. Am J Obstet Gynecol
9. Stevens BJ, Gibbins S, Yamada J, et al. The premature infant pain profile-revised (PIPP-R): initial validation and feasibility. Clin J Pain
10. Belfort MB, Pearce EN, Braverman LE, et al. Low iodine content in the diets of hospitalized preterm infants. J Clin Endocrinol Metab
11. Comrie JD, Helm JM. Common feeding problems in the intensive care nursery: maturation, organization, evaluation, and management strategies. Semin Speech Lang
1997; 18:239–260.quiz 261.
12. Lee JH, Chang YS, Yoo HS, et al. Swallowing dysfunction in very low birth weight infants with oral feeding desaturation. World J Pediatr
13. Briere CE, McGrath J, Cong X, et al. State of the science: a contemporary review of feeding readiness in the preterm infant. J Perinat Neonatal Nurs
14. Gennattasio A, Perri EA, Baranek D, et al. Oral feeding readiness assessment in premature infants. MCN Am J Matern Child Nurs
15. Gewolb IH, Bosma JF, Taciak VL, et al. Abnormal developmental patterns of suck and swallow rhythms during feeding in preterm infants with bronchopulmonary dysplasia. Dev Med Child Neurol
16. Lessen BS. Effect of the premature infant oral motor intervention on feeding progression and length of stay in preterm infants. Adv Neonatal Care
17. Sheppard JJ, Fletcher KR. Evidence-based interventions for breast and bottle feeding in the neonatal intensive care unit. Semin Speech Lang
18. Arvedson J, Clark H, Lazarus C, et al. Evidence-based systematic review: effects of oral motor interventions on feeding and swallowing in preterm infants. Am J Speech Lang Pathol
19. Takwoingi Y, Hopewell S, Tovey D, et al. A multicomponent decision tool for prioritising the updating of systematic reviews. BMJ
20. Moher D, Liberati A, Tetzlaff J, et al. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. Int J Surg
21. Wiley Online Library, Higgins JP, Green S. Cochrane Handbook for Systematic Reviews of Interventions. Vol. 5. 2008.
22. Davidson MB, Castellanos M, Kain D, et al. The effect of self-monitoring of blood glucose concentrations on glycated hemoglobin levels in diabetic patients not taking insulin: a blinded, randomized trial. Am J Med
23. Brok J, Thorlund K, Gluud C, et al. Trial sequential analysis reveals insufficient information size and potentially false positive results in many meta-analyses. J Clin Epidemiol
24. Brok J, Thorlund K, Wetterslev J, et al. Apparently conclusive meta-analyses may be inconclusive—trial sequential analysis adjustment of random error risk due to repetitive testing of accumulating data in apparently conclusive neonatal meta-analyses. Int J Epidemiol
25. Thorlund K, Devereaux PJ, Wetterslev J, et al. Can trial sequential monitoring boundaries reduce spurious inferences from meta-analyses? Int J Epidemiol
26. Goldman AI, Hannan PJ. Optimal continuous sequential boundaries for monitoring toxicity in clinical trials: a restricted search algorithm. Stat Med
27. Wetterslev J, Thorlund K, Brok J, et al. Estimating required information size by quantifying diversity in random-effects model meta-analyses. BMC Med Res Methodol
28. Thorlund K, Engstrøm J, Wetterslev J, Brok J, Imberger G, Gluud C User manual for trial sequential analysis (TSA). Copenhagen Trial Unit
. Available at: http://wwwctudk/tsa/files/tsa_manualpdf
. Accessed 2011.
29. Bache M, Pizon E, Jacobs J, et al. Effects of pre-feeding oral stimulation on oral feeding in preterm infants: a randomized clinical trial. Early Hum Dev
30. Boiron M, Da Nobrega L, Roux S, et al. Effects of oral stimulation and oral support on non-nutritive sucking and feeding performance in preterm infants. Dev Med Child Neurol
31. Fucile S, Gisel E, Lau C. Oral stimulation accelerates the transition from tube to oral feeding in preterm infants. J Pediatr
32. Fucile S, Gisel EG. Sensorimotor interventions improve growth and motor function in preterm infants. Neonatal Netw
33. Fucile S, Gisel EG, Lau C. Effect of an oral stimulation program on sucking skill maturation of preterm infants. Dev Med Child Neurol
34. Lu MY CY, Hong AL, Wang J. The clinical studies for oral motor intervention on feeding performance in preterm infants. Nurs Rehabil
35. Lyu T-C, Zhang Y-X, Hu X-J, et al. The effect of an early oral stimulation program on oral feeding of preterm infants. Int J Nurs Sci
36. Lyu TC ZY, Hu XJ, Cao Y, et al. Effect of oral stimulation and nonnutritive sucking on feeding performance in preterm infants. J Nurs (China)
37. Xiao Wen WW, Xu YF, Ni YL. The effect of oral motor intervention in feeding progression and performance on preterm infants. Chin Mod Doctor
38. Zhang YX LT, Hu XJ, Shi P, et al. Effect of nonnutritive sucking and oral stimulation on feeding performance in preterm infants. A randomized controlled trial. Pediatr Crit Care Med
39. Martin JA, Osterman M, Sutton PD. Are preterm births on the decline in the United States? Recent data from the National Vital Statistics System. NCHS Data Brief
40. Amaizu N, Shulman R, Schanler R, et al. Maturation of oral feeding skills in preterm infants. Acta Paediatr
41. Gewolb IH, Vice FL. Maturational changes in the rhythms, patterning, and coordination of respiration and swallow during feeding in preterm and term infants. Dev Med Child Neurol
42. Gewolb IH, Vice FL, Schwietzer-Kenney EL, et al. Developmental patterns of rhythmic suck and swallow in preterm infants. Dev Med Child Neurol
43. Harding C, Frank L, Van Someren V, et al. How does non-nutritive sucking support infant feeding? Infant Behav Dev
44. Stumm S, Barlow SM, Estep M, et al. Respiratory distress syndrome degrades the fine structure of the non-nutritive suck in preterm infants. J Neonatal Nurs
45. Heron M, Hoyert DL, Murphy SL, et al. Deaths: final data for 2006. Natl Vital Stat Rep
46. Zarbock SF. Hospital discharge of the high-risk neonate. Home Care Provid