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Research Article: Observational Study

Total time of operation is a risk factor of stroke-associated pneumonia in acute ischemic stroke patients with intra-arterial treatment

Liu, Rui MDa; Li, Wei MDb; Li, Yaoyang MDb; Han, Yunfei MDa; Ma, Minmin MD, PhDa; Zhu, Wusheng MD, PhDa; Li, Min MDa; Dai, Qiliang MDa; Cao, Yuezhou MDb; Xu, Gelin MD, PhDa; Liu, Xinfeng MD, PhDb,*

Section Editor(s): Chu., Danny

Author Information
doi: 10.1097/MD.0000000000003958
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1 Introduction

Intra-arterial therapy (IAT), either through local delivery of thrombolytic agents or mechanical thrombectomy, has provided credible evidence for the treatment of acute ischemic stroke (AIS) within 6 h after symptom onset.[1–3] Complications frequently complicate stroke and have a significant impact on prognosis, length of stay, and healthcare costs.[4–6] Stroke-associated pneumonia (SAP) is the most common cause of mortality following acute stroke and is also associated with poor functional outcome.

Although the results of previous studies demonstrated the effectiveness of IAT, SAP may be still an important reason causing a higher totally cost and a relatively low proportion of patients had a modified Rankin score (mRS) of 0 to 2 at the 90-day follow-up assessment.[1,7] Identifying patients with the highest risk of developing SAP may be helpful for treatment and prevention.

Traditionally, SAP is considered to be secondary to aspiration.[8–10] Several risk factors for SAP have been identified, such as older age, male sex, diabetes mellitus, hypertension, atrial fibrillation, congestive heart failure, chronic obstructive pulmonary disease, pre-existing dependency, stroke severity, stroke subtypes, and dysphagia.[11–16] Previous studies also showed that the incidence of SAP depends on the setting. It varies between 10% and 57% for patients treated in intensive care units and between 4% and 12% for patients treated exclusively in stroke units.[17] Some studies have provided evidence for stroke-induced immunodepression.[18,19]

We conducted this study to determine how frequently patients with AIS underwent IAT get SAP, and identify predictors of SAP in patients underwent IAT.

2 Subjects and Methods

2.1 Study design

The study population was consisted of consecutive patients with AIS within 6 h from the symptom onset prospectively enrolled in the Nanjing Stroke Registry Program[20] from March 2010 to July 2014. The study was approved by the institutional review boards at Jinling Hospital, and all participants or their surrogates provided written informed consent. The study was carried out in accordance with the Helsinki Declaration. The potential intra-arterial treatment (IAT) were evaluated based on computed tomographic (CT) angiography, magnetic resonance angiography, or digital-subtraction angiography, and a score of 2 or higher on the National Institutes of Health Stroke Scale (NIHSS) score in all patients. IAT includes arterial catheterization with delivery of a thrombolytic agent, mechanical thrombectomy, or both. Radiological and angiographic imaging was reviewed. We documented location of vascular occlusion, thrombolysis in cerebral infarction (TICI) flow pre and postprocedure, and procedural complications. Data were collected by the study physicians through review of medical records and neurological examination. Neurological status was evaluated on a daily basis. Pretreatment NIHSS and 90-day mRS were assessed by certified stroke.[1,21]

Exclusion criteria including: history of life-threatening allergy to contrast medium, not just rash; oral anticoagulant therapy with International Normalized Ratio > 3.0, known hemorrhagic diathesis, or coagulation factor deficiency; baseline platelet count < 30,000/μL; creatinine ≥ 3 mg/dL; illness with <1 year anticipated life expectancy.[22]

After intravenous thrombolysis within 36 h, the patient with any bleeding signs in CT scan and with a deterioration of 4 or more points in the NIHSS score should be diagnosed as symptomatic intracerebral hemorrhage.[22,23]

In this study, the medical diagnosis of pneumonia requires a new and persistent infiltration or solidifies presence on at least 1 chest x-ray, or at least 2 serial chest X-rays in cases of underlying lung disease. Some clinical signs should be combined, including fever, leukocytosis, or leukopenia, and mental status change who are older than 70 years. Two of the following signs should be added to above signs, including rales, cough, purulent sputum or sputum characteristics change, and gas exchange worsening.[22]

IAT efficacy was ultimately evaluated based on restored flow postprocedure, a TICI score of 2b or 3, and the absence of periprocedural complications.[24] Good outcome was defied as mRS ≤ 2 at 90 days, and mortality was defied as mRS = 6 at 90 days. These parameters were acquired by telephone interview with patients, relatives, or family doctors.

2.2 Statistical analysis

The Statistical Product and Service Solutions (SPSS, IBM Corporation, Armonk, NY) program (version 22) was used to analyze the data. Depending on the distribution of data, continuous variables are expressed as means (standard deviation) and medians (interquartile ranges [IQRs]). Nominal and categorical variables and percentages for categorical variables are expressed as absolute numbers and percentages. The correlation between categorical variables was determined by Chi-squared test. A t test between continuous variables and a Mann–Whitney test between scores were performed. The odds ratios (ORs) were estimated by adjusted logistic regression. A P < 0.1 were entered into the logistic regression model. In order to study the ability of SAP for mortality prediction, we calculated Kaplan–Meier survival curves and compared by the log-rank test. Hazard ratios were reported. A P < 0.05 was considered as significant.[22]

3 Results

3.1 Patient characteristics

Of 165, 102 (61.8%) hospitalized patients with AIS underwent IAT in the study period. There were 36 women (35.3%) and 66 men (64.7%). The median interval from stroke onset to puncture was 253.6 min (range, 58–363 min). Mean age of the study patients was (64.2 ± 10.5) years (range, 40–85 years). The median NIHSS score was 15 (IQR, 10.0–19.3).

Before IAT, 28 patients (27.5%) were treated with intravenous alteplase. Six patients (5.9%) were given in no intervention. Actual intra-arterial therapy was performed in 96 of 102 patients (94.1%). Intra-arterial thrombolytic agents were given to 36 patients (35.3%). Retrievable stents were used in 65 patients (63.7%). Acute cervical carotid stenting used as a simultaneous second revascularization procedure was performed in 12 patients (11.8%).

3.2 Factors influencing SAP

Twenty-two intra-arterial treated patients (21.6%) were diagnosed as pneumonia during hospitalization (95% confidence interval [CI], 14.7–29.4). The average duration of hospital stays is 10.8 ± 9.0 days. The clinical symptom of patients with pneumonia (NIHSS score, 18) was more serious than those without pneumonia (NIHSS score, 9) (P = 0.004). In addition, patients with pneumonia were older (69.2 vs 62.9 years, respectively; P = 0.011). Symptom of dysphagia was occurred more (86.4% vs 15%, respectively; P < 0.001) and Glasgow Coma Scale (GCS) score was lower (9 vs 13, respectively; P < 0.001) in patients with SAP. The operation time of patients with SAP was longer (P < 0.001). No association was found between pneumonia and TOAST classification, and no association was found between pneumonia and responsible vessels (Table 1). In a univariate analysis, SAP was significantly influenced by 5 variables (Table 1): age ≥ 70 years (crude OR, 2.8; 95% CI, 1.1–7.4; P = 0.037), the severity of neurologic deficits (NIHSS score ≥ 15; crude OR, 4.156; 95% CI, 1.4–12.4; P = 0.010), admission GCS ≤ 8 (crude OR, 2.769; 95% CI, 1.0–7.6; P = 0.048), total time of operation (crude OR, 1.045; 95% CI, 1.020–1.071; P < 0.001), and symptom of dysphagia (crude OR, 16.029; 95% CI, 5.066–50.717; P < 0.001). The multiple logistic regression analysis showed only symptom of dysphagia (adjust OR, 12.051; 95% CI, 3.457–5.610; P < 0.001) and total time of operation (adjust OR, 1.040; 95% CI, 1.009–1.071; P = 0.011) were identified as independent predictors of SAP (Table 2, Figs. 1 and 2).

Table 1
Table 1:
Univariate analysis of variables associated with in-hospital SAP after AIS.
Table 2
Table 2:
Multivariable analysis of parameters associated with in-hospital SAP after AIS.
Figure 1
Figure 1:
(A) Univariate analysis of parameters associated with in-hospital SAP after AIS; (B) multivariate analysis of parameters associated with in-hospital SAP after AIS. AIS = acute ischemic stroke, GCS = Glasgow Coma Scale, IAT = intra-arterial treatment, NIHSS = National Institutes of Health Stroke Scale, SAP = stroke-associated pneumonia.
Figure 2
Figure 2:
Scatter diagram of stroke-associated pneumonia distribution according NIHSS score and total time of operation. NIHSS = National Institutes of Health Stroke Scale.

3.3 Clinical outcomes

Patients without SAP were more likely to occur a favorable outcome (mRS ≤ 2) than those with SAP (48.8% vs 31.8%, respectively; P = 0.031). Four of all 80 patients without SAP and 3 of all 22 patients with SAP died in hospital (5.0% vs 13.6%, respectively; P = 0.169). After 3 months, the mortality rates for patients without SAP were lower than those with SAP (8.75% vs 22.7%, respectively; P < 001). Patients with SAP had stable or improved deficits after AIS with IAT (P < 0.001) (Figs. 3 and 4).

Figure 3
Figure 3:
Modified Rankin scale scores at 90 d in the 2 groups. SAP = stroke-associated pneumonia.
Figure 4
Figure 4:
Kaplan–Meier survival curve depicting 90-d survival of patients with acute ischemic stroke underwent intra-arterial treatment. SAP = stroke-associated pneumonia.

4 Discussion

Our results show that age, NIHSS score, GCS score, duration time of operation, and symptom of dysphagia were significantly associated with SAP in patients with AIS with IAT within 6 h. The associations of age, NIHSS score, and GCS score with SAP were attenuated after adjusting for presence of dysphagia and total time of operation. This study also demonstrated that patients with AIS underwent IAT with SAP had a significantly poorer functional outcome and increased mortality rate.

With the publication of MR CLEAN, ESCAPE, EXTEND-IA, and SWIFT PRIME trials, IAT has been considered as a potently effective treatment for AIS. The studies suggest that IAT should be offered to patients who have documented occlusion in the anterior circulation. The rate of TICI 2b/3 is 61.76% in our study, and the difference between SAP group and no SAP group was no significant. In the previous IAT studies, the rate of TICI 2b/3 was reported >70%,[1,25–28] which can be explained that the patients included in this study were different from those in the above studies. About 16.6% patients studied in this research were confirmed to be posterior circulation occlusion.

Several research has revealed that SAP is in connection with a higher rates of mortality and less favorable prognosis.[29] In this study, the incidence of SAP was remarkably higher than in other stroke unit studies (21.6% vs 4–12%, respectively). One explanation might be in some of the comparable stroke unit studies, the neurologic deficit of patients was less severe than in our study (NIHSS score, 7–8, 9 vs 15, respectively).[29–31] A second potential explanation may be our study including data from patients’ stay in the stroke unit and the peripheral ward, and prospective study reported that rate of SAP (19%) performed in even less severely affected patients (NIHSS score, 5) in a mixed setting.[22]

Several risk factors for SAP have been identified. Consistent with the prior studies, in-hospital SAP was significantly associated with older age, present history of atrial fibrillation, congestive heart failure, chronic obstructive pulmonary disease and smoking, prestroke dependence, admission NIHSS score, GCS score, dysphagia, blood glucose, and stroke subtypes.[11–16] Some clinical score were developed and validated for predicting SAP after AIS, such as A2DS2 clinical score and 34-point AIS-APS.[15,16] In our study, the SAP group had obvious older age, higher NIHSS score, lower GCS score, longer total time of operation, and higher rate of symptom of dysphagia. We also observed the tendency of higher rate of male sex and present history of atrial fibrillation in SAP group, but the difference was not significant compared with no SAP group. Because of the small sample size, the patients rolled in this study have not found any of congestive heart failure and chronic obstructive pulmonary disease. The most interesting result in this study is that only symptom of dysphagia and total time of operation were found identified as independent predictors of SAP in this study. One explanation of that is may be longer operation time coursing longer time to get reconstruction of brain blood flow, which may decrease the chances of favorable outcomes. After that older patients need more time for surgery because of the tortuous artery, and SAP is occurs more in older patients.

In our study, SAP patients’ deficits were stable or improved. Compared with SAP group, survival of SAP group was prolonged.[22] In other studies, mortality evaluated at 30 days or at time of discharge from hospital was between 10.1% and 37.3%. Mortality evaluated after 12 months was reported to be between 49% and 60.1%.[5,8,10,17] In this study, the Kaplan–Meier 90-day survival estimate (12.7%) was lower than MR CLEAN study (21%),[1] but higher than ESCAPE study (10%).

Our study had some limitations. First, SAP is a complex disease, and various factors play—or are assumed to play—a role in pathogenesis. As an observational study, we cannot rule out the possibility that some nonincluded baseline variables might have some impact on the development of in-hospital SAP after AIS. In addition, our study is a small sample size study. Several confirmed risk factors for SAP have not been identified in this research. Second, as we only have information on new-onset SAP during hospitalization without documentation of the exact date, our data allow no conclusion as to whether patients with a longer length of stay to develop pneumonia or whether diagnosis of pneumonia leads to a longer hospitalization.

In conclusion, this study suggested that beside dysphagia, total time of operation is a risk factor of SAP in patients with AIS with IAT. SAP is associated with poor functional outcome and higher mortality after AIS underwent IAT. Our results reinforce the need for identifying patients with AIS underwent IAT at high risk for pneumonia.


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complication; mechanical thrombolysis; outcome; pneumonia; stroke

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