The intravesical administration of bacillus Calmette-Guérin (BCG), an attenuated live strain of Mycobacterium bovis, has become a mainstay of adjunctive therapy for superficial bladder cancer.139,191,194 Although usually well tolerated, both local and systemic BCG-related complications may occur following instillation.103 While these events are uncommon, with a cumulative incidence lower than 5%,106,199 its wide range of presentations and potential severity poses a challenge for the clinician. However, much of our knowledge on the infectious complications related to intravesical BCG therapy comes from individual case reports, thus hampering a comprehensive understanding of this entity.
The pathogenic mechanisms underlying the development of complications following BCG instillation remain not fully understood, and considerable debate exists about whether it represents a form of hypersensitivity reaction—on the basis of the histologic finding of granulomas in the absence of recoverable microorganisms—or an active mycobacterial infection—since some authors have demonstrated viable bacilli in a variety of tissues.51 In addition, the predisposing factors for developing an intravesical BCG-related complication are not well known. It has been suggested that some conditions may result in the hematogenous dissemination of the bacilli (that is, disruption of the urothelial barrier due to traumatic urinary catheterization, early instillation after transurethral resection [TUR] of bladder cancer, or concurrent urinary tract infection), but there are still limited data on other potential risk factors, namely the presence of underlying immunosuppression.93,230
In the present study we aimed at analyzing a large cohort of patients who underwent intravesical BCG instillation in our institution during a 6-year period in order to determine the incidence, risk factors, and outcome of systemic BCG infection. An exhaustive review and pooled analysis of the cases of BCG infection following intravesical immunotherapy reported in the literature since 1975 is also presented, with particular focus on the nature of complication (local or systemic), therapeutic approach, and outcome.
PATIENTS AND METHODS
Study Setting and Data Collection
This study was performed at the University Hospital “12 de Octubre” (Madrid, Spain), a 1300-bed tertiary care center with a reference population that ranged from 536,450 to 405,577 inhabitants during the study inclusion period (December 31, 2003, to October 31, 2009). By means of a retrospective search of the institutional database we identified a total of 315 patients who received ≥1 instillations of intravesical BCG (Connaught strain, 81 mg in 50 mL of sterile saline) as adjunctive treatment of superficial bladder cancer throughout this period. We excluded 59 patients (18.7%) due to the lack of clinical data, so 256 patients were finally included. All the patients were followed for at least 6 months after the last intravesical instillation. The need for specific informed consent was waived by the institutional review board due to the strictly retrospective and noninterventional nature of the investigation.
The following variables were retrospectively assessed by medical record review using a standardized data collection form: demographics; number of TURs prior to the first BCG instillation; overall number of BCG instillations; time interval between the last TUR and the first instillation; prior history of tuberculin skin test (TST) or active tuberculosis disease; alcohol and smoking habits; comorbid conditions; and immunosuppression. We recorded the total leukocyte and lymphocyte counts just before the first instillation as a proxy for the patient’s immune status.230 A number of additional variables were also recorded for those patients who developed systemic BCG infection (as defined below): time interval between the first BCG instillation and the diagnosis of the complication; type of infection; presence of miliary pattern on chest imaging studies (simple radiography or computerized tomography [CT] scan); histologic and microbiologic findings; treatment; and outcome. One of these cases has been previously published.120
Definitions Used in the Institutional Cohort Study
Systemic BCG infection was defined by the presence of miliary tuberculosis (clinical presentation consistent with active tuberculosis associated to a typical miliary pattern on chest imaging), hepatitis, nephritis, lymphocytic meningitis, arthritis, or osteomyelitis, following ≥1 instillations of intravesical BCG, responding to antituberculosis treatment, and with no alternative diagnosis. Microbiologic (positivity for Mycobacterium tuberculosis complex by culture or by polymerase chain reaction [PCR] assay) and/or histopathologic evidence of mycobacterial infection (that is, caseating granulomas in biopsy specimens) were not deemed necessary for diagnosis. We also included in such definition the occurrence, within the first 4 hours after BCG instillation, of persistent fever (≥38°C for more than 72 h) and night sweating, with rapid defervescence after initiation of antituberculosis treatment, and no alternative clinical cause or microbiologic documentation (other than the isolation of M. bovis in urine samples).106,172Immunosuppression was defined by the presence of ≥1 of the following conditions: long-term corticosteroid therapy (≥5 mg daily of prednisone or equivalent for >2 wk), use of other immunosuppressive or cytostatic agents within the previous 6 months, advanced human immunodeficiency virus (HIV) infection (CD4+ T-cell count ≤0.200 × 103 cells/μL), neutropenia (absolute neutrophil count ≤0.500 × 103 cells/μL), other primary or secondary immunodeficiencies, and asplenia.
Literature Review and Pooled Analysis of Cases
We conducted a computer-based MEDLINE (National Library of Medicine, Bethesda, MD) search with the terms “intravesical instillation” and “bacillus Calmette-Guérin” and “BCG infection” or “tuberculosis, miliary” or “complications” to identify literature pertaining to the subject published between January 1975 and April 2013. Overall, we retrieved 390 articles (including case reports, case series, review articles, and randomized clinical trials [RCTs]). First, we excluded papers in languages other than English, French, Spanish, Italian, or Portuguese (28 articles). After a detailed evaluation, we also excluded those papers that clearly referred to other subjects, which overall added up to 137 (antitumor mechanism of BCG [26 articles], efficacy of intravesical BCG as treatment for bladder cancer [38 articles], comparative analyses of different doses and strains of BCG [4 articles], alternative treatments for superficial bladder cancer [12 articles], comparative analyses between BCG and other intravesical agents [10 articles], alternative routes for the administration of BCG [9 articles], detection of M. bovis in asymptomatic patients after receiving intravesical BCG [10 articles], and potential efficacy of isoniazid [INH] or quinolones for prevention of BCG complications [3 articles], among others). We excluded the case previously reported from our institution, which had been included into our cohort study.120 We also excluded 9 literature reviews with no original data, 7 studies based on animal models, 4 guidelines, 1 article based on a pooled analysis of cases (thus making impossible to characterize each patient separately), and 1 comment to a previous article. In addition, we reviewed the bibliographies of the selected articles for additional relevant references, adding 34 further articles. Thirty-three references (13.9%) were not included due to the impossibility to obtain the complete article. Therefore, we included a total of 201 articles comprising 271 cases of BCG infection after intravesical instillation published since 1975.1–14,17–19,21–31,33–39,41–49,51–71,73–80,82–84,87–97,99,101,102,104,105,107–119,121–138,140–151,153–168,170,171,173–180,182–189,192,193,195–202,205–208,210–214,218,219,221–223,225–232 Of these, 170 were individually detailed cases, and 31 were summarized in reports containing 2–13 cases. All data shown here were extracted from these case reports and case series, but occasionally articles lacked relevant clinical or microbiologic data. Thus, denominators for various analyses differed, as the data were included only if the results for a particular variable were explicitly stated in the report.
Definitions Used in the Literature Review
For the purpose of the pooled analysis we considered as systemic complications any of the following scenarios: disseminated BCG infection (further defined as 1) sepsis with fever, hypotension, multiorgan failure and coagulopathy; 2) miliary tuberculosis; or 3) fever associated with bone marrow and/or liver involvement and/or dyspnea and hypoxemia, but with no demonstration of miliary pattern on chest imaging); persistent fever (as previously defined); and any organ involvement excluding the genitourinary tract. We considered as local complications those exclusively involving the genitourinary tract. In all cases, the authors should specify that alternative diagnoses had been reasonably excluded. Immunosuppression was defined by the aforementioned criteria.
Quantitative data were shown as the mean ± standard deviation (SD), or the median with absolute or interquartile (Q1–Q3) ranges, as appropriate. Qualitative variables were summarized with absolute and relative frequencies and 95% confidence intervals (CIs). Comparisons between groups were made by the Student unpaired t test (or Mann–Whitney U test when the assumption of normality did not hold) for continuous variables, and with the chi-square test (or the Fisher exact test) for proportions. Given the long time frame of this study, an era effect was included in the mortality analysis by dividing the literature review period in 2 (cases reported in 1975–1999 [n = 128] and those reported in 2000–2013 [n = 154]). All significance tests were 2-tailed, and differences were considered significant at p < 0.05. The statistical analysis was carried out using SPSS v. 15.0 (SPSS Inc, Chicago, IL).
Institutional Cohort Study
Overall, we analyzed 256 patients (227 males; mean age: 67.9 ± 8.7 yr) treated with intravesical BCG at our institution. Eleven of them developed a systemic BCG infection throughout the follow-up, yielding a cumulative incidence of 4.3% (95% CI: 2.4–7.5%). The time interval between the last instillation and the onset of the complication was 8.3 ± 6.5 days. As detailed in Table 1, the forms of BCG infection included miliary tuberculosis (6 cases, 1 of them associated with hepatitis), persistent fever (2 cases), lymphocytic meningitis, arthritis, and tubulointerstitial nephritis (1 case each). M. bovis was isolated in urine specimens from 3/11 patients (27.3%). A 3-drug antituberculosis regimen was initiated in all but 1 patient, with a favorable outcome in 9/10 cases (90%) after completion of a 6-month course. One patient with miliary tuberculosis died due to a multiorgan failure despite treatment with INH, rifampicin (RIF) and ethambutol (EMB) (Case 11). The patient diagnosed with tubulointerstitial nephritis was treated only with systemic corticosteroids (intravenous boluses of methylprednisolone for 3 consecutive d, followed by oral prednisone [1 mg/kg per d] with progressive tapering over 4 mo), as the pathogenic mechanism was deemed to be immune-mediated (Case 10); only a partial recovery of renal function was obtained, as detailed elsewhere.120
We found no significant differences in terms of demographics, comorbidities, or previous diagnosis of active or latent tuberculosis infection between patients with and without systemic BCG infection. There were either no significant differences in the mean number of TURs prior to the first BCG instillation, the overall mean number of instillations, or the time interval between the last TUR procedure and the first instillation (Table 2).
A total of 12 patients (4.7% of the entire cohort) had some form of immunosuppression at the time of the first instillation, with no significant differences between groups (p = 0.389). In the group with systemic BCG infection, 1 of 11 patients (9.1%) had undergone a splenectomy. In the group without BCG infection, 10 of 245 patients (4.1%) had a condition conferring some degree of immunosuppression: long-term corticosteroid therapy (5 cases), other immunosuppressive therapies (2 cases), hypogammaglobulinemia (3 cases) and splenectomy (1 case). Finally, there were no differences between both groups in total leukocyte and lymphocyte counts at the time of the first BCG instillation either (see Table 2).
Pooled Analysis of the Literature
We jointly analyzed a total of 282 patients (11 new cases from our institution and 271 from the literature). Basic demographics (age and sex) were available for 276 and 279 patients, respectively: 96.1% were men, with a mean age at diagnosis of 66.6 ± 11.0 years. Major comorbidities are detailed in Table 3.
The different forms of BCG infection are shown in Table 4. Disseminated infection was the most common manifestation, reported in 97 cases (34.4%). In detail, this complication consisted of miliary tuberculosis in 71 cases (25.2%), fever associated to bone marrow and/or liver infiltration in 19 (6.7%), and sepsis with multiorgan failure in 7 (2.5%). Four patients (1.4%) had persistent fever responding to antituberculosis treatment as the unique clinical manifestation.
Osteoarticular involvement was seen in 56 patients (19.9%), mostly in form of mono-, oligo- or polyarthritis affecting the extremities and, in some cases, the temporomandibular articulation. Reactive arthritis (arthritis associated with genitourinary symptoms and ocular involvement such as conjunctivitis or uveitis) was diagnosed in 16 patients (5.7%), usually positive for human leukocyte antigen (HLA)-B27 (9 of 14 cases with available data). Prosthetic material infection was described in 5 patients (1.8%), with the hip being the affected joint. Thoracolumbar spondylodiscitis constituted another rare complication of BCG instillation (10 cases [3.5%]), occasionally associated with paraspinal or psoas abscesses (4 and 3 patients, respectively). Muscle abscess constituted the only clinical manifestation in 5 cases (1.8%), with involvement of psoas, iliac, adductor, and perineal groups.
Mycotic aneurysms and pseudoaneurysms were seen in 16 patients (5.7%), affecting abdominal (12 cases) and thoracic aorta (1 case) and carotid and femoral arteries (2 cases each); 1 patient presented simultaneously with mycotic aneurysms in the abdominal aorta and the superficial femoral artery. Some aneurysms complicated with aorto-enteric (4 cases) and carotid-cutaneous fistulas (1 case) and secondary psoas abscess (2 cases). There were 2 cases of infection of vascular bypass grafts (axilo-femoral and femoro-femoral).
Isolated ocular involvement, outside the setting of reactive arthritis, was rare (9 cases [3.2%]) and included panuveitis (4 cases), granulomatous anterior uveitis (3 cases), endophthalmitis and autoimmune retinopathy (1 case each). Visual loss was present in all cases, and the funduscopic examination revealed choroidal tubercles in 3 of 4 patients with panuveitis.
Hepatitis (reported as isolated manifestation in 16 cases [5.7%]) presented with fever, anorexia, jaundice and alteration of liver function tests, typically with a cholestatic pattern of serum liver tests (gamma-glutamyl transpeptidase [GGT] range: 110–885 IU/L; alkaline phosphatase [ALP] range: 116–1460 IU/L); liver biopsy revealed granulomas in all cases.
Local (genitourinary) complications were reported in 66 patients (23.4%), and encompassed different manifestations. Bladder involvement was present in 17 patients (5.9%): 13 of them had a solitary bladder ulcer on cystoscopy (with caseating granulomas found in all 7 cases with histologic analysis), whereas the remaining 4 presented with cystitis and sterile pyuria. Penile lesions (17 cases [5.9%]) consisted of nodules, papules, plaques or ulcers, with or without inguinal lymph node enlargement. Prostate involvement was seen in 11 patients (3.5%), either as diffuse prostatic enlargement (5 cases) or focal nodule or abscess (6 cases). Kidney parenchymal involvement (10 patients [3.5%]) manifested in form of nephritis in 5 cases (with demonstration of granulomatous inflammation in all but 1 case) or renal masses on abdominal imaging in the remaining 5. Finally, epididymo-orchitis with painless testicular enlargement, abscesses or cutaneous fistula was reported in 10 cases (3.5%).
Only 5 patients (1.8%) had an obvious cause of immunosuppression at the time of BCG instillation: active treatment with cytostatic drugs, HIV infection, long-term corticosteroid therapy, kidney transplantation, and splenectomy (1 case each). There was no difference in the prevalence of underlying immunosuppression between patients with or without disseminated BCG infection (3.1% vs 1.1%; p = 0.343). A previous diagnosis of M. tuberculosis infection, either latent infection or active disease, was present in 19 cases (6.7%).
Most patients had undergone previous TURs (161 of 166 evaluable cases), with a median of 1.0 (Q1–Q3 range: 1.0–2.0) procedures prior to the first instillation. The median time interval between both therapeutic measures was 30.0 (Q1–Q3 range: 21.0–41.0) days. The median number of instillations (reported in 279 cases) was 6.0 (Q1–Q3 range: 4.0–9.0), and the median time interval between the last instillation and the onset of infection (reported in 182 cases) was 13.5 (Q1–Q3 range: 2.0–195.0) days.
There were no significant differences between those patients with disseminated and nondisseminated BCG infection in the mean number of TURs before the instillation (1.0 in both groups; p = 0.975) or the median number of BCG instillations (6.0 in both groups; p = 0.650). However, we found a lower interval between the last instillation and the onset of the complication in the group with disseminated BCG infection as compared to the rest of the pooled patients (median interval: 2.0 vs 42.0 d; p < 0.001).
Some degree of bladder mucosal disruption due to genitourinary tract manipulation prior to BCG instillation, other than TUR, was reported in 36 patients (12.8%), and included photodynamic therapy and fulguration (11 cases), intravesical chemotherapy administration (9 cases), local radiotherapy (6 cases), nephroureterectomy or cystectomy (8 cases), and nephrostomy, double J catheter insertion, and transurethral removal of urethral stone (1 case each). Patients with previous bladder mucosal disruption were more likely to develop nondisseminated forms of BCG infection, specifically spondylodiscitis (11.1% vs 2.4%; p = 0.027).
Specimens for microbiologic diagnosis were obtained in 246 patients (87.2%), whereas histologic examination was performed in 185 (65.6%). Only in 17 patients (6.0%) neither microbiologic nor histologic investigations were conducted, and the diagnosis was solely based on clinical manifestations and response to antituberculosis treatment. Such approach was more frequent in patients with isolated ocular involvement (29.4% vs 1.5%, p < 0.001). The diagnostic performance rates for acid-fast bacilli staining, conventional mycobacterial culture (Middlebrook and Löwenstein-Jensen media) and PCR-based assays were 25.3%, 40.9% and 41.8%, respectively (Table 5). Microbiologic-based diagnosis was more common in patients with nondisseminated forms of BCG infection (53.0% vs 37.8%; p = 0.024). The histologic examination revealed granulomatous inflammation in 86.3% of biopsied cases.
Treatment and Outcome
Data on therapeutic management were available for 274 cases. Overall, 269 patients (98.2%) received some form of treatment: antituberculosis therapy alone (127 patients [46.4%]), antituberculosis therapy combined with systemic corticosteroids (55 [20.1%]), antituberculosis therapy and surgery (43 [15.7%]), nonsteroidal antiinflammatory drugs (NSAIDs) alone (22 [8.0%]), corticosteroid therapy alone (12 [4.4%]), surgical therapy alone (7 [2.6%]), and other regimens (3 [1.1%]).
The drug regimens used in the 226 patients that received antituberculosis therapy are detailed in Table 6. The most common regimen consisted of INH, RIF and ETB with or without pyrazinamide (PZA), followed by the combination of INH and RIF (40.2% and 28.3% of patients, respectively). The median treatment duration in 171 evaluable patients was 6.0 (Q1–Q3 range: 4.0–9.0) months.
A surgical approach, either alone or in combination with other therapy, was performed in 51 patients (18.6%): aneurysm or pseudoaneurysm resection and debridement with prosthetic graft interposition or extra-anatomic bypass (17 cases); orchiectomy (10 cases); drainage of muscle or cutaneous abscess (6 cases); replacement or permanent resection arthroplasty, or debridement with prosthesis retention (6 cases); vertebral laminectomy, drainage of epidural abscess, and spinal stabilization (5 cases, all of them with spondylodiscitis); nephrectomy (3 cases); and subcutaneous nodulectomy and intestinal resection (1 case each).
Five patients (1.8%) received no specific treatment: 2 patients dying before treatment could be initiated (due to massive hematemesis secondary to an aortoesophageal fistula, and multiorgan failure in the setting of a miliary tuberculosis, respectively), 1 patient with a pulmonary infiltrate and granulomas in the lung biopsy specimens whose symptoms resolved spontaneously, 1 patient with a miliary pattern on the chest radiograph with complete radiologic resolution after 3 months of interruption of BCG instillations, and 1 patient with severe rhabdomyolysis and secondary renal failure—attributed to BCG therapy due to the close temporal association in the absence of an alternative cause—that resolved after interruption of intravesical instillations and initiation of renal replacement therapy.
The different therapeutic approaches used according to the type of BCG infection are shown in Table 7. The patients diagnosed with disseminated BCG infection more commonly received antituberculosis drugs (93.7% vs 76.5%; p < 0.001) and adjuvant systemic corticosteroids (43.0% vs 15.9%; p < 0.001), whereas those with reactive arthritis were frequently treated only with NSAIDs (50.0% vs 5.4%; p < 0.001).
Status at hospital discharge was reported in 258 patients (91.5%), with a median follow-up of 12.0 (Q1–Q3 range: 6.0–23.0) months in 44 evaluable cases. All-cause and BCG infection-attributable mortality rates were 6.9% (18/258) and 5.4% (14/258), respectively. Attributable mortality was higher for patients aged ≥65 years at diagnosis (7.4% vs 2.1%; p = 0.091), those with disseminated BCG infection (9.9% vs 3.0%; p = 0.040), and those with vascular involvement (16.7% vs 4.6%; p = 0.064). We found no differences in mortality according to the study period (5.0% in cases reported in 1975–1999 vs 5.8% in those reported in 2000–2013; p = 0.801) or the use of a PZA-containing antituberculosis regimen (5.9% vs 7.4%; p = 0.546). The causes of attributable death were multiorgan failure (10 patients), rupture of mycotic aneurysm, liver failure, massive hematemesis secondary to an aortoesophageal fistula, and perforation of anastomotic leak after surgical repair of a mycotic aneurysm (1 case each). Nineteen patients (7.4%) developed some form of long-term complication following treatment, including permanent loss of visual acuity (5 cases), chronic arthralgia (5 cases), end-stage renal disease (4 cases), chronic respiratory insufficiency and retractile bladder (1 case each). The outcome was deemed satisfactory in the remaining 221 patients (85.7%).
Among the 36 patients for whom such information was specifically reported, only in 2 (5.6%) the scheduled regimen of intravesical instillations was restarted once the treatment for the BCG infection had been completed. The first case was a 47-year-old male with a firm subcutaneous nodule that was treated with surgical excision and RIF plus INH for a nonspecified period of time, with continuation of intravesical BCG therapy and no new complications after 1 year. The second case was a 35-year-old female diagnosed with peritoneal tuberculosis and treated with INH, RIF, and EMB for 2 months, followed by dual treatment with INH and RIF for 7 further months; 10 months after the end of antituberculosis therapy, intravesical BCG administration was completed to 6 doses, with an uneventful 2-year follow-up period.
Throughout the decades, BCG immunotherapy remains as the standard of care in patients with high-risk superficial bladder cancer, being the most commonly used and the most effective intravesical agent for this malignancy.40,190 BCG effectively eradicates existing carcinoma in situ, decreases the likelihood of tumor recurrence, and reduces the odds of disease progression after TUR.204 Although its intravesical instillation usually exhibits a favorable safety profile, since it contains viable attenuated mycobacteria the potential for serious adverse events exists.103 To date, most of the data on BCG infection come from single case reports, with only a few observational cohort studies aimed at establishing its incidence.106,167,199,230 An additional drawback lies on the lack of consensus diagnostic criteria accurate enough to differentiate true infection from self-limited post-instillation symptoms, and there is not a common disease classification consistently used through the literature. In an attempt to shed some light on this uncommon complication, we have performed the present institutional cohort study and pooled analysis of previous case reports.
The immune response triggered by the adhesion of BCG to the bladder urothelium activates the production of cytokines and promotes local migration of polymorphonuclear leukocytes and macrophages, ultimately leading to the death of tumor cells.181,220 As an inherent event following this inflammatory challenge, most of patients experience irritative voiding (urgency, dysuria, frequency and, occasionally, hematuria) and flu-like symptoms, including low-grade fever and malaise for less than 24–48 hours after instillation.103,220 Such a phenomenon, far from being considered as adverse events, has been regarded as a marker for adequate antitumor effect exerted by the BCG.16
Apart from these self-limiting symptoms, BCG immunotherapy is long known to be associated to a protean constellation of localized and systemic complications. A variety of terms have been traditionally used to describe the occurrence of adverse events—other than merely mechanical complications during the catheterization procedure—in patients undergoing intravesical BCG instillations, such as “tuberculosis,” “BCGitis,” “hypersensitivity reaction,” “granulomatous complication,” or simply, “M. bovis infection.” Such terminology heterogeneity is likely mirroring the incomplete understanding of the pathogenic mechanisms underlying these complications. In an attempt to systematize the present review we categorized as “local complications” those confined to the structures of the genitourinary tract and, therefore, in direct contact with the bacilli. On the opposite, the term “systemic complication” implies some degree of distant hematogenous dissemination (that is, miliary tuberculosis) or cross-reactivity between tissue self-antigens and mycobacterial antigens (that is, reactive arthritis, also called Reiter syndrome).
Previous studies have reported an incidence of systemic BCG infection ranging from 3%199 to 7%.167 Lamm et al included over 2600 patients having BCG therapy and found an incidence of 1% for local and 4.8% for systemic complications,106 a figure comparable to that we have found in our series. A recent RCT showed a cumulative incidence at 3 years of 30.6%, although the definition used for “systemic side effect” included the occurrence of influenza-like symptoms and general malaise; in fact, the incidence rates for sepsis and pulmonary BCG infection were notably lower (0.3% and 0.4%, respectively).20 The variability of clinical features and the fact that the onset of the complication can be delayed months to years after the last instillation (median of 13.5 d in our review) may hinder prompt diagnosis and treatment. The longest interval between BCG administration and occurrence of the complication (epididymo-orchitis) ever reported was 17 years.178 Mavrogenis et al diagnosed a case of spondylodiscitis 11 years after the end of therapy.127 In this regard, it has been documented the recovery of bacilli from the urinary tract various months after the completion of intravesical therapy.217 As in the review by Gonzalez et al,70 we have found that the interval between the instillation and the onset of the complication was lower in patients with disseminated infection (mainly miliary tuberculosis), whereas late-presentation disease usually involved the genitourinary tract and other localized sites.
Disseminated BCG infection, in the form of miliary tuberculosis, sepsis, or fever associated with organ involvement, constituted the most commonly reported form of BCG infection in the literature, accounting for one-third of cases. However, it is noteworthy the wide range of clinical manifestations that emerged from our review, including anecdotal reports of prosthetic joint infection,23,69,75,164,184 parotid gland tuberculosis,49 endogenous endophthalmitis with infiltrative retinitis,111 or lymphocytic meningitis (present Case 1). Of note, BCG infection had been missed from the initial differential diagnosis in most of these cases until microbiologic documentation of M. bovis was obtained by culture or PCR assay, thus emphasizing the need of maintaining a high index of clinical suspicion based on a previous history of BCG exposure.
To date, the predisposing factors for developing BCG infection after intravesical instillation remains to be well characterized. Historically, poor technique during BCG administration, with traumatic instillation or concurrent urinary tract infection, has been regarded as a major risk factor.106,155,199,221 Unfortunately, accurate information on the urinary catheterization procedure was provided for only 56 of 282 patients included in the pooled analysis, preventing us from separately analyzing this variable. Interestingly, we have found that the existence of breaches in the bladder mucosal barrier at the initiation of instillations acts as a specific predisposing factor for the occurrence of spondylodiscitis, with cases following prostatectomy,28 transurethral removal of urethral stone,101 and nephroureterectomy.145 In addition, the history of progressive bladder cancer often present in these patients may lead to the misdiagnosis of spinal bone metastasis.157 The spread of the mycobacteria from the lower urinary tract to the spine via the Batson venous plexus may be the pathogenic mechanism underlying this association.147 The precocity in the onset of intravesical instillations after the immediately preceding TUR would in theory favor that the bacilli gain access to lymphatics and bloodstream through the disruption of the urothelial integrity.106 Nevertheless, in our institutional cohort there were no differences in the time interval between those procedures according to the subsequent development of complications. The mean time between the last TUR and the first BCG instillation both in our experience and in the pooled literature review was around 30 days, similar to that recommended in current guidelines, which indicate a delay of 2 to 3 weeks between TUR and instillation to assure the healing of the mucosa at resection sites.32 A recent RCT aimed at comparing different maintenance regimens of BCG (one-third vs full dose for 1 or 3 yr) failed to demonstrate significant differences either in the frequency of toxicity or in the rate of treatment discontinuation due to side effects between the study arms.20 Although such data were not available in our institutional cohort study, we find no differences in the number of BCG instillations between those with or without infection. Overall, these findings suggest that the odds for developing BCG-related complications likely depend mostly on the host’s characteristics (that is, presence of major bladder mucosal damage or certain underlying conditions) or on the accuracy of the instillation technique, rather than on the BCG dose, the number of treatment courses or the interval elapsed since the last preceding TUR.
Since cigarette smoking is a well-recognized risk factor for urinary tract cancer, many patients undergoing BCG immunotherapy are or have been heavy smokers and face a significant risk for diffuse atherosclerotic disease. We have identified 19 cases of vascular complication following intravesical BCG instillations, most of them in form of mycotic aneurysm or pseudoaneurysm17,34,35,83,87,173–175,183,227 or infection of vascular prosthetic grafts.183,187 This form of disease may occur either by hematogenous seeding of the vasa vasorum of the arterial wall or as a result of direct extension from a contiguous infected site, typically spondylodiscitis36 or perivascular lymph nodes.67,183 The vascular infection often follows a protracted course with low-grade fever, malaise and weight loss83,126; the median time interval between the previous instillation and the diagnosis was 16.7 months in our review. The prognosis is dismal, with an attributable mortality rate of 15.8%.126,173,196 Although it is usually unclear whether this complication develops on a pre-existing atherothrombotic aneurysm or emerges from the primary infection of a normal vessel with subsequent aneurysm formation, clinicians should be particularly aware of this complication in patients undergoing BCG instillations and previous evidence of atherosclerotic disease in various vascular territories or multiple cardiovascular risk factors. In this sense, some authors have proposed the use of prophylaxis and close imaging follow-up in patients with recent vascular graft insertion.83,183
It has been theoretically argued that intravesical BCG should not be administered in the setting of immunosuppression since its antitumor therapeutic efficacy could be decreased due to the impaired immunomodulating effect.230 In addition, safety concerns regarding the potential for systemic BCG infection have usually led to avoid the use of this therapy in immunosuppressed hosts. Nevertheless, the data on this specific population are scarce. Sun et al203 reviewed the experience with intravesical BCG after kidney transplantation and identified 7 cases through the literature,98,152,209,224 concluding that the tolerance was overall good, with no BCG-related complications after a median follow-up period of 17 months; on the other hand, the recurrence rate of the bladder cancer was higher than that expected in the general population. No graft rejection following instillation was observed, a relevant finding since Th1 responses, which play a crucial role in alloreactivity, have been involved in the antitumor activity of BCG. In our institutional cohort study we found that nearly 5% of the patients exerted various degrees of immunosuppression at BCG instillation, mainly long-term steroid therapy and hypogammaglobulinemia. There was a numerically higher prevalence of immunosuppressive conditions in those patients who subsequently developed a disseminated BCG infection (9.1% vs 4.1%), although not reaching statistical significance. Moreover, the underling comorbidity in the only patient included in the former group was splenectomy, a condition not directly affecting the cell-mediated immune response and, therefore, with a presumably little impact on the odds of mycobacterial infection. To our knowledge, only 1 further study has been specifically focused on the potential impact of other forms of immunosuppression on the occurrence of BCG infection. Yossepowitch et al reviewed 697 patients treated with BCG instillations at their institution and found that 24 of them had an underlying condition affecting immune function (mainly oral and inhaled steroid use and lymphoproliferative disorders). Only 1 patient on steroids experienced self-limited febrile disease, and both the response rate and safety profile were comparable to those reported in the general immunocompetent population.230 In the pooled literature analysis we identified an even lower prevalence of immunosuppression (1.8%), including administration of corticosteroids35 and cytostatic drugs,223 HIV infection,104 and previous kidney transplantation with nonfunctioning graft.31 This group did not suffer from more severe forms of BCG infection as compared to the remaining patients. Although limited by the low number of subjects and the heterogeneity in the nature of the underlying conditions, this evidence supports that intravesical BCG should be deemed a viable therapeutic option in immunosuppressed hosts.
With aging, the immune system progressively declines in both its innate and adaptive arms. Elderly individuals would theoretically face an increased risk of complications after receiving intravesical BCG, and some authors have proposed that maintenance BCG therapy should be given with caution in patients aged 70 years or older.86 However, a retrospective study did not find any difference in the occurrence of severe complications in patients over 80 years, with a relatively lower disease-free rate in this group as compared to younger patients.100 We neither found an apparent association between age and the development of systemic infection in our institution. Furthermore, the mean age of patients diagnosed with BCG infection in the pooled literature analysis (66.6 yr) was close to that of patients undergoing an uneventful course of therapy both in our experience and in other series.230 On the other hand, it has been suggested that a positive purified protein derivative (PPD) test before instillation could predict the subsequent occurrence of systemic side effects and, presumably, a better antitumor activity.15,216 Unfortunately, data on previous PPD tests were reported in only 12 cases (4 of them with a positive result37,110,136 [and present Case 3]), thus preventing us from drawing any conclusion on this point. However, previous reports do not contain clear recommendations regarding this issue.81
The exclusion of other entities and the prompt response to antituberculosis treatment should be considered the cornerstones of diagnosis of BCG infection since cultures often remain negative, as observed both in our institutional experience and in the literature review (culture positivity rate of 40.9% in the pooled analysis). It has been suggested that such low sensitivity could be explained by the rapid control of bacillary replication by the host’s immune system, due to the attenuated virulence of BCG and the preexisting delayed-type hypersensitivity that leads to granuloma formation.31,70 Our study underlies that the tissue biopsy specimen is the best sample for BCG detection by conventional culture (positive in 42.2% of cases with culture documentation), although the lack of comparative studies prevents any firm conclusion on the potentially higher diagnostic yield of newer PCR-based assays in such or other samples. This fact, together with the high identification rate of granulomas in the histologic examination, makes the tissue sample particularly relevant in the diagnostic workup for BCG infection. Conversely, the positive predictive value of isolating M. bovis from urine samples is notably low, as each 50 mL of reconstituted suspension for intravesical instillation contains over 2–8 × 108 colony forming units of BCG, most of which will be washed out with the first post-instillation void.20 Nevertheless, a urine culture should be routinely performed in patients with suspected BCG infection in order to rule out the occurrence of urinary tract infection caused by conventional uropathogens, which represents a far more common cause of fever following genitourinary tract manipulation.172 The advent of molecular-based techniques (that is, PCR assay) has progressively modified our understanding of the pathogenesis of BCG infection, as it has lead to the increase in the overall rate of microbiologic diagnosis seen throughout the period encompassed by our review (42.1% in cases reported in 1975–1999 vs 52.5% in those reported in 2000–2013).85 The detection of M. tuberculosis complex DNA in locations distant from the genitourinary tract, such as lung,29,31,104,211 liver,63,108,222 aortic wall,227 or periprosthetic hip joint164 samples, reinforces the hypothesis that the direct invasion and hematogenous spread of BCG underlie the development of the complication, rather than a mere hypersensitivity reaction. In keeping with this rationale, M. bovis has been recovered from the synovial fluid of patients with polyarthritis,13,219 a manifestation generally deemed as immune-mediated. Chest imaging also play a crucial role in diagnosing disseminated BCG infection and the present review suggests that CT scan should always be preferred over conventional radiography, as the latter technique failed to reveal a miliary pattern in about one-quarter of the patients eventually found to have miliary tuberculosis.11,29,37,39,46,63,85,117,141,142,177,214,231
Our review underscores the great heterogeneity in the therapeutic approach to the intravesical BCG-related complications, and the absence of a clear consensus on the indication for antituberculosis therapy, the preferred regimen, or its duration. As previously commented, the early-onset of irritative voiding and flu-like symptoms should not prompt the administration of specific therapy other than symptomatic.106,172 On the contrary, the use of antituberculosis drugs, either alone or in combination with corticosteroids or surgery, is common in both systemic and local forms of BCG infection (82.2% of cases in the pooled analysis). Its role in cases of polyarthritis or reactive arthritis remains less clear, as most of them were successfully managed with NSAIDs.13,25,42,57,124,134,136,148,154,159,167,179,207,210 Nevertheless, the aforementioned occasional documentation of M. bovis in articular samples in some cases13,219 should be taken account in order to reevaluate the initiation of antituberculosis therapy, particularly if the evolution is not satisfactory.
As an attenuated derivative of M. bovis, BCG is intrinsically resistant to PZA. In addition, the different BCG strains (Connaught, Tice and RIVM) have also been demonstrated to be resistant to cycloserine.50 Despite this susceptibility profile, a PZA-containing regimen was used in as many as 15% of reported cases in the literature, with no apparent impact on the outcome. The most commonly prescribed drug combination in our institution consisted of INH and RIF for 6 months, with a 2-month intensive phase including ETB. Such regimen is based on the standard recommended therapy for M. tuberculosis infection, avoiding the use of PZA due to the lack of in vitro activity and its potential for additive liver toxicity. In the absence of specifically designed RCTs some authors have proposed an alternative regimen consisting of INH for 3 months for patients with high-degree fever as only clinical manifestation.20,72,106,169,217 Nevertheless, the rationale underlying this recommendation seems obscure since if the direct invasion hypothesis is accepted, the therapeutic regimen should include various active antimycobacterial drugs. The Connaught BCG strain is highly susceptible to fluoroquinolones,50 and some patients have been successfully treated with regimens containing ofloxacin,70,74 ciprofloxacin,174 moxifloxacin,64 and levofloxacin,52,117 [and present report Cases 1 and 9], occasionally instead of RIF or INH. This promising preliminary experience should be analyzed in future studies. On the other hand, the lower liver toxicity of these drug combinations has to be carefully counterbalanced against the well-known risk of tendinopathy associated with the long-term use of fluoroquinolones.233 Not surprisingly, corticosteroids were frequently associated with the antituberculosis treatment in patients with disseminated BCG infection, mainly miliary tuberculosis, emphasizing again the probable dual role of both hypersensitivity reaction and active infection in the pathogenesis of systemic complications.1,52,169
Despite the exhaustive nature of the present review, no clear recommendations can be made regarding the potential risk posed by the reintroduction of intravesical instillations in subjects previously diagnosed with a BCG-related complication, although detailed data on follow-up were provided only for a minority of cases (12.8%). Most authors consider a preceding systemic BCG infection to be an absolute contraindication for restarting the course of therapy.106,169,172 In a recent RCT the decision of whether instillations were to be only postponed or definitely interrupted was left to the treating physician according to the severity of symptoms.20 In that line, it is generally assumed that therapy might be safely resumed after certain nonsevere manifestations such as isolated fever, reactive arthritis or uveitis. However, in our institution we administered no further instillations in patients with prolonged, otherwise unexplained fever, as we hypothesize that such scenario should be actually regarded as a systemic form of BCG infection (present Cases 4 and 6). Of note, 1 of the only 2 cases in which the scheduled regimen of instillations was restarted had been diagnosed with a systemic infection (peritoneal tuberculosis), with an uneventful outcome.197
Overall, the prognosis of BCG infection is good, although we have found nonnegligible rates of attributable mortality (5.4%) and long-term disability (7.4%). Interestingly, 3 factors linked with higher mortality (that is, age at diagnosis ≥65 yr, disseminated infection and vascular involvement) emerged from our pooled analysis, although some of these associations showed only borderline significance. No previous studies have specifically assessed factors predicting poor outcome in this complication. The attributable mortality rate in the study by Gonzalez et al was slightly higher (11.6% [5/43]), with all the fatal cases having been diagnosed with disseminated BCG infection or mycotic aneurism.70 By considerably increasing the sample size, our results are in line with those preliminary findings. Since we have not been able to identify risk factors predicting the development of complications in the overall population undergoing intravesical BCG therapy, maybe prevention strategies should be targeted to those subjects at the highest risk for poor evolution in the case of eventual BCG infection (older patients and those with pre-existing atherothrombotic aneurysms). A RCT reported a significant decrease in the rate of systemic complications requiring antituberculosis treatment with the prophylactic use of ofloxacin after each instillation.32 Other RCT with INH given as prophylaxis failed to demonstrate a protective effect.215 Furthermore, our review revealed cases of miliary tuberculosis,163 hepatitis,208 or aortoenteric fistula56 despite the prophylactic administration of INH.
The present study has a number of limitations. As with all pooled analysis of previously reported cases, our data are heterogeneous in origin and to some extent incomplete (that is, reporting on traumatic instillation or eventual reintroduction of BCG therapy). We cannot exclude publication bias favoring cases with a more severe course. In the series by Lamm et al106 isolated fever was the most frequent complication in patients treated with intravesical BCG (2.9%), although we have only identified 2 other single case reports in the literature,87,192 thus suggesting some degree of underreporting of this presentation. The rate of long-term complications may be underestimated due to insufficient follow-up. In addition, the low incidence of systemic BCG infection in our institutional series (11 patients) made comparisons between groups prone to a type 2 error because of inadequate statistical power.
On the other hand, ours is one of the largest series of patients with BCG infection coming from a single institution and recruited throughout a relatively short period, therefore ensuring homogeneous diagnostic and therapeutic approaches. In addition, to our knowledge this is the most comprehensive review and pooled case report analysis to date, not limited to English literature. The review by Gonzalez et al was performed 1 decade ago and included only 43 cases from United States and Canada,70 whereas more recent reviews have been focused on specific forms (that is, miliary tuberculosis,31 reactive arthritis,210 or vascular infection56,226). Due to the rarity of BCG infection and the difficulty to obtain a large multicenter series of cases in a prospective way, we think that this approach is a valid way to clarify some aspects regarding this occasionally life-threatening condition.
In conclusion, since apparently there are no obvious predictors that could help clinicians to identify patients at risk for developing BCG infection after intravesical instillations, and in view of the protean clinical manifestations of this complication, a high index of suspicion remains essential in order to promptly initiate antituberculosis therapy. Although this entity has an overall favorable prognosis, uncertainties about the safety of restarting intravesical BCG instillations frequently lead to noncompliance with the scheduled therapy regimen, thus jeopardizing the prognosis of the underlying malignancy. Prospective, multicenter studies should be performed to precisely address the risk factors for this complication as well as its optimal therapeutic approach. Meanwhile, we summarize our recommendations (Table 8) and propose a diagnostic and treatment algorithm for patients with suspected BCG infection based on our experience and the present literature review (Figure 1).
The authors thank the personnel of the Library of the Hospital Universitario “12 de Octubre” for their kind assistance in collecting the literature.
1. Adami M, Marsteller I, Mazzucchelli L, et al. Granulomatous hepatitis after intravesical bacillus Calmette-Guerin treatment. Scand J Infect Dis. 2011;43:55–57.
2. Aljada IS, Crane JK, Corriere N, et al. Mycobacterium bovis BCG causing vertebral osteomyelitis (Pott’s disease) following intravesical BCG therapy. J Clin Microbiol. 1999;37:2106–2108.
3. Alvarez-Mugica M, Gomez JM, Vazquez VB, et al. Pancreatic and psoas abscesses as a late complication of intravesical administration of bacillus Calmette-Guerin for bladder cancer: a case report and review of the literature. J Med Case Rep. 2009;3:7323.
4. Andres E, Kuhnert C, Perrin AE, et al. [Sepsis syndrome and bone marrow granulomatosis after intravesical instillation of BCG]. Presse Med. 1999;28:1753–1754.
5. Armstrong RW. Complications after intravesical instillation of bacillus Calmette-Guerin: rhabdomyolysis and metastatic infection. J Urol. 1991;145:1264–1266.
6. Aslan G, Sevinc C, Tuna B, et al. Penile lesion with inguinal adenopathy after intravesical Bacillus Calmette-Guerin instillation therapy. Indian J Urol. 2013;29:70–72.
7. Audigier C, Nesme P, Perol M, Guerin JC. [Rare pulmonary complication in intravesical BCG treatment]. Rev Mal Respir. 2000;17:679–681.
8. Aust TR, Massey JA. Tubercular prostatic abscess as a complication of intravesical bacillus Calmette-Guerin immunotherapy. Int J Urol. 2005;12:920–921.
9. Baloira Villar A, Agüero Balbin R, Bustamante Ruiz A, et al. [Miliary tuberculosis secondary to intravesical instillation of Calmette-Guerin bacilli]. Med Clin (Barc). 1992;99:158.
10. Baniel J, Lev Z, Engelstein D, Servadio C. Penile edema and meatal ulceration after intravesical instillation with bacillus Calmette-Guerin. Urology. 1996;47:932–934.
11. Barankiewitz I, Manor H, Strauss S. Miliary lung disease induced by intravesical Bacillus Calmette-Guerin treatment. Eur Radiol. 1999;9:1933.
12. Bartolome Pacheco MJ, Martinez-Taboada VM, Blanco R, et al. Reactive arthritis after BCG immunotherapy: T cell analysis in peripheral blood and synovial fluid. Rheumatology (Oxford). 2002;41:1119–1125.
13. Belmatoug N, Levy-Djebbour S, Appelboom T, et al. [Polyarthritis in 4 patients treated with intravesical BCG-therapy for carcinoma of the bladder]. Rev Rhum Ed Fr. 1993;60:162–166.
14. Bijol V, Mendez GP, Nose V, Rennke HG. Granulomatous interstitial nephritis: a clinicopathologic study of 46 cases from a single institution. Int J Surg Pathol. 2006;14:57–63.
15. Bilen CY, Inci K, Erkan I, Ozen H. The predictive value of purified protein derivative results on complications and prognosis in patients with bladder cancer treated with bacillus Calmette-Guerin. J Urol. 2003;169:1702–1705.
16. Bohle A, Brandau S. Immune mechanisms in bacillus Calmette-Guerin immunotherapy for superficial bladder cancer. J Urol. 2003;170:964–969.
17. Bornet P, Pujade B, Lacaine F, et al. Tuberculous aneurysm of the femoral artery: a complication of bacille Calmette-Guerin vaccine immunotherapy: a case report. J Vasc Surg. 1989;10:688–692.
18. Borrego Hernando J, Parra Muntaner L, Rivas Escudero JA, et al. [Perineal tuberculous abscess. Presentation of a clinical case]. Arch Esp Urol. 1997;50:810–811.
19. Bouhabel A, Takoucht F, Bousbia W, et al. Bladder tuberculosis after BCG therapy. Saudi J Kidney Dis Transpl. 2008;19:80–81.
20. Brausi M, Oddens J, Sylvester R, et al. Side effects of Bacillus Calmette-Guerin (BCG) in the treatment of intermediate- and high-risk Ta, T1 papillary carcinoma of the bladder: results of the EORTC Genito-Urinary Cancers Group randomised phase 3 study comparing one-third dose with full dose and 1 year with 3 years of maintenance BCG. Eur Urol. 2013. Jul 24 [epub ahead of print]. doi: 10.1016/j.eururo.2013.07.021.
21. Briceno-Garcia EM, Gomez-Pardal A, Alvarez-Bustos G, et al. Tuberculous orchiepididymitis after BCG therapy for bladder cancer. J Ultrasound Med. 2007;26:977–979.
22. Cameo MI, Lezcano MA, Gil D, Blas M. [Urinary tuberculosis caused by Mycobacterium bovis BCG variety, following intravesical instillation]. Enferm Infecc Microbiol Clin. 2010;28:134–135.
23. Chazerain P, Desplaces N, Mamoudy P, et al. Prosthetic total knee infection with a bacillus Calmette Guerin (BCG) strain after BCG therapy for bladder cancer. J Rheumatol. 1993;20:2171–2172.
24. Cheung JM, Hou SS, Yip SK, Ng CF. An unusual cause of retention of urine after intravesical Bacillus Calmette-Guerin therapy for superficial bladder cancer. Hong Kong Med J. 2011;17:492–494.
25. Chevrel G, Zech C, Miossec P. Severe uveitis followed by reactive arthritis after Bacillus Calmette-Guerin therapy. J Rheumatol. 1999;26:1011.
26. Chiner E, Larramendi C, Carbonell C, et al. [Respiratory distress and multiple organ failure after intravesical instillation of BCG]. An Med Interna. 1991;8:349–351.
27. Chowdhury AR, Dey RK. Penile tuberculosis following intravesical Bacille Calmette-Guerin immunotherapy. Indian J Urol. 2013;29:64–66.
28. Civen R, Berlin G, Panosian C. Vertebral osteomyelitis after intravesical administration of bacille Calmette-Guerin. Clin Infect Dis. 1994;18:1013–1014.
29. Cobas Paz A, Garcia Tejedor JL, Gonzalez Piñeiro A, Fernandez-Villar A. [Miliary tuberculosis due to BCG in an asymptomatic patient: initial onset or a condition not yet described?]. Arch Bronconeumol. 2010;46:394–395.
30. Colebatch AN, Mounce KE. Mycobacterium bovis discitis as a complication of intravesical Bacillus Calmette-Guerin therapy. J. Clin Rheumatol. 2010;16:74–75.
31. Colmenero JD, Sanjuan-Jimenez R, Ramos B, Morata P. Miliary pulmonary tuberculosis following intravesical BCG therapy: case report and literature review. Diagn Microbiol Infect Dis. 2012;74:70–72.
32. Colombel M, Saint F, Chopin D, et al. The effect of ofloxacin on bacillus calmette-guerin induced toxicity in patients with superficial bladder cancer: results of a randomized, prospective, double-blind, placebo controlled, multicenter study. J Urol. 2006;176:935–939.
33. Colombo R, Consonni P, Nava L, et al. [Chronic granulomatous prostatitis:echographic features in patients submitted to immunoprophylaxis/therapy with BCG]. Arch Ital Urol Nefrol Androl. 1991;63:147–149.
34. Coscas R, Arlet JB, Belhomme D, et al. Multiple mycotic aneurysms due to Mycobacterium bovis after intravesical bacillus Calmette-Guerin therapy. J Vasc Surg. 2009;50:1185–1190.
35. Costiniuk CT, Sharapov AA, Rose GW, et al. Mycobacterium bovis abdominal aortic and femoral artery aneurysms following intravesical bacillus Calmette-Guerin therapy for bladder. Cardiovasc Pathol. 2010;19:e29–e32.
36. Dahl T, Lange C, Odegard A, et al. Ruptured abdominal aortic aneurysm secondary to tuberculous spondylitis. Int Angiol. 2005;24:98–101.
37. Dammert P, Boujaoude Z, Rafferty W, et al. Fever of unknown origin and pancytopenia caused by culture-proven delayed onset disseminated bacillus Calmette-Guerin (BCG) infection after intravesical instillation. BMJ Case Rep. 2013. doi: 10.1136/bcr-2013-008949.
38. De Diego A, Rogado MC, Prieto M, et al. Disseminated pulmonary granulomas after intravesical bacillus Calmette-Guerin immunotherapy. Respiration. 1997;64:304–306.
39. de la Iglesia Fanjul I, de Castro Losa MR, Mourad F, et al. [Disseminated infection due to Mycobacterium bovis alter intravesical instillation of BCG]. An Med Interna. 2007;24:514–515.
40. de Reijke TM, Kurth KH, Sylvester RJ, et al. European Organization for the Research and Treatment of Cancer-Genito-Urinary Group. Bacillus Calmette-Guerin versus epirubicin for primary, secondary or concurrent carcinoma in situ of the bladder: results of a European Organization for the Research and Treatment of Cancer–Genito-Urinary Group Phase III Trial (30906). J Urol. 2005;173:405–409.
41. de Saint Martin L, Boiron C, Poveda JD, Herreman G. [Generalized BCG infection after intravesical instillations of Calmette-Guerin bacillus]. Presse Med. 1993;22:1352–1356.
42. de Torres JA, Lorente JA, Villegas G, Lience E. [Polyarthritis as a complication of the treatment with intravesical BCG]. Med Clin (Barc). 1994;102:556.
43. Dederke B, Riecken EO, Weinke T. A case of BCG sepsis with bone marrow and liver involvement after intravesical BCG instillation. Infection. 1998;26:54–57.
44. Del Castillo Duran Y, Santos Bodi F, Castander Serentill D, et al. Tuberculosis miliar in a patient treated with intravesical instillations of bacillus Calmette-Guerin. Med Intensiva. 2006;30:116–119.
45. Del Prete R, Ditonno P, Mosca A, et al. BCG septicemia after radical cystectomy: a rare postoperative complication following BCG therapy. J Infect. 2002;45:112–114.
46. Dentale N, Manfredi R. [Respiratory and uro-genital infection with bacillus of Calmette-Guerin following administration as a local adjuvant therapy of bladder carcinoma]. Infez Med. 2010;18:259–266.
47. Deresiewicz RL, Stone RM, Aster JC. Fatal disseminated mycobacterial infection following intravesical bacillus Calmette-Guerin. J Urol. 1990;144:1331–1333.
48. Devlin M, Deodhar A. Arthritis as a complication of intravesical BCG vaccine. BMJ. 1994;308:1638.
49. Diaz C, Baldo C, Martin A, et al. [Parotid tuberculosis following intravesical BCG instillation: a case report]. Acta Otorrinolaringol Esp. 2003;54:129–133.
50. Durek C, Rüsch-Gerdes S, Jocham D, Bohle A. Sensitivity of BCG to modern antibiotics. Eur Urol. 2000;37(suppl 1):21–25.
51. Eichel L, Erturk E, Disant’Agnese A. Drug resistant Mycobacterium bovis cystitis following intravesical bacillus Calmette-Guerin treatment. J Urol. 1999;162:2096.
52. Elkabani M, Greene JN, Vincent AL, et al. Disseminated Mycobacterium bovis after intravesicular bacillus Calmette-Guerin treatments for bladder cancer. Cancer Control. 2000;7:476–481.
53. Erol A, Ozgur S, Tahtali N, et al. Bacillus Calmette-Guerin (BCG) balanitis as a complication of intravesical BCG immunotherapy: a case report. Int Urol Nephrol. 1995;27:307–310.
54. Ersoy O, Aran R, Aydinli M, et al. Granulomatous hepatitis after intravesical BCG treatment for bladder. Indian J Gastroenterol. 2006;25:258–259.
55. Falkensammer C, Gozzi C, Hager M, et al. Late occurrence of bilateral tuberculous-like epididymo-orchitis after intravesical bacille Calmette-Guerin therapy for superficial bladder carcinoma. Urology. 2005;65:175.
56. Farber A, Grigoryants V, Palac DM, et al. Primary aortoduodenal fistula in a patient with a history of intravesical therapy for bladder cancer with bacillus Calmette-Guerin: review of primary aortoduodenal fistula without abdominal aortic aneurysm. J Vasc Surg. 2001;33:868–873.
57. Faus S, Martinez Montauti JM, Puig L. Reiter’s syndrome after administration of intravesical bacille Calmette-Guerin. Clin Infect Dis. 1993;17:526–527.
58. Fernandez Cañabate E, Longoni Merino M. [Systemic infection secondary to intravesical BCG instillation]. Farm Hosp. 2006;30:317–319.
59. Fernandez Jimenez Ortiz H, Gomez Marco JJ, Rojo Zamanillo I, et al. [Miliary tuberculosis in a patient treated with BCG intravesical instillation]. Actas Urol Esp. 2007;31:783–784.
60. Foster DR. Miliary tuberculosis: a complication of intravesical BCG treatment. Australas Radiol. 1998;42:167–168.
61. Fradet V, Gaudreau C, Perrotte P, et al. Management of hepatic granulomatous tuberculosis complicating intravesical BCG for superficial bladder cancer. Can Urol Assoc J. 2007;1:269–272.
62. French CG, Hickey L, Bell DG. Caseating granulomas on the glans penis as a complication of bacille calmette-guerin intravesical therapy. Rev Urol. 2001;3:36–38.
63. Gao CQ, Mithani R, Leya J, et al. Granulomatous hepatitis, choroiditis and aortoduodenal fistula complicating intravesical Bacillus Calmette-Guerin therapy: case report. BMC Infect Dis. 2011;11:260.
64. Garcia Baldovi M, Perez-Crespo M, Onrubia J, et al. Granulomatous balanitis after intravesical Bacille Calmette-Guerin instillation. Actas Dermosifiliogr. 2013;104:251–252.
65. Garip A, Diedrichs-Mohring M, Thurau SR, et al. Uveitis in a patient treated with Bacille-Calmette-Guerin:possible antigenic mimicry of mycobacterial and retinal antigens. Ophthalmology. 2009;116:2457–2462.
66. Gatfosse M, Vignal T, Legraverend JM. [Systemic B. C. G. infection after intravesical B. C. G. therapy of in situ carcinoma of the bladder]. Rev Med Interne. 1990;11:181–182.
67. Geldmacher H, Taube C, Markert U, Kirsten DK. Nearly fatal complications of cervical lymphadenitis following BCG immunotherapy for superficial bladder. cancer. 2001;68:420–421.
68. George VK, Russell GL, Harrison BD, Green NA. Tuberculous epididymo-orchitis following intravesical BCG. Br J Urol. 1990;66:101–102.
69. Gomez E, Chiang T, Louie T, et al. Prosthetic joint infection due to Mycobacterium bovis after intravesical instillation of Bacillus Calmette-Guerin (BCG). Int J Microbiol. 2009;2009:527208. doi: 10.1155/2009/527208.
70. Gonzalez OY, Musher DM, Brar I, et al. Spectrum of bacille Calmette-Guerin (BCG) infection after intravesical BCG immunotherapy. Clin Infect Dis. 2003;15:140–148.
71. Gottke MU, Wong P, Muhn C, et al. Hepatitis in disseminated bacillus Calmette-Guerin infection. Can J Gastroenterol. 2000;14:333–336.
72. Grange JM. Complications of bacille Calmette-Guerin (BCG) vaccination and immunotherapy and their management. Commun Dis Public Health. 1998;1:84–88.
73. Graziano DA, Jacobs D, Lozano RG, Buck RL. A case of granulomatous hepatitis after intravesical bacillus Calmette-Guerin administration. J Urol. 1991;146:1118–1119.
74. Griggs H, Cammarata SK. Acute mental changes in a 68-year-old man with bladder cancer. Chest. 1998;114:621–623.
75. Guerra CE, Betts RF, O’Keefe RJ, Shilling JW. Mycobacterium bovis osteomyelitis involving a hip arthroplasty after intravesicular bacille Calmette-Guerin for bladder. Clin Infect Dis. 1998;27:639–640.
76. Guex-Crosier Y, Chamot L, Zografos L. Chorioretinitis induced by intravesical Bacillus Calmette-Guerin (BCG) instillations for urinary bladder carcinoma. Klin Monbl Augenheilkd. 2003;220:193–195.
77. Gupta RC, Lavengood R Jr, Smith JP. Miliary tuberculosis due to intravesical bacillus Calmette-Guerin therapy. Chest. 1988;94:1296–1298.
78. Hakim S, Heaney JA, Heinz T, et al. Psoas abscess following intravesical bacillus Calmette-Guerin for bladder cancer: a case report. J Urol. 1993;150:188–189.
79. Hammadeh MY, Dutta SN, Worrall JG, Morgan RJ. Acute reactive polyarthritis after intravesical bacillus Calmette-Guerin instillation. Br J Urol. 1995;76:811–812.
80. Hansen CP, Mortensen S. Epididymo-orchitis and Reiter’s disease. Two infrequent complications after intravesical bacillus Calmette-Guerin therapy. Scand J Urol Nephrol. 1997;31:317–318.
81. Hanson K. BCG installations for bladder cancer and latent tuberculosis infection. Urologic Nursing. 2002;22:132–134.
82. Harada H, Seki M, Shinojima H, et al. Epididymo-orchitis caused by intravesically instillated bacillus Calmette-Guerin: genetically proven using a multiplex polymerase chain reaction method. Int J Urol. 2006;13:183–185.
83. Harding GE, Lawlor DK. Ruptured mycotic abdominal aortic aneurysm secondary to Mycobacterium bovis after intravesical treatment with bacillus Calmette-Guerin. J Vasc Surg. 2007;46:131–134.
84. Harving SS, Asmussen L, Roosen JU, Hermann G. Granulomatous epididymo-orchitis, a rare complication of intravesical bacillus Calmette-Guerin therapy for urothelial cancer. Scand J Urol. 2009;43:331–333.
85. Heemstra KA, Bossink AW, Spermon R, et al. Added value of use of a purified protein derivative-based enzyme-linked immunosorbent spot assay for patients with Mycobacterium bovis BCG infection after intravesical BCG instillations. Clin Vaccine Immunol. 2012;19:974–977.
86. Heiner JG, Terris MK. Effect of advanced age on the development of complications from intravesical bacillus Calmette-Guerin therapy. Urol Oncol. 2008;26:137–140.
87. Hellinger WC, Oldenburg WA, Alvarez Vascular S. Vascular and other serious infections with Mycobacterium bovis after bacillus of Calmette-Guerin therapy for bladder cancer. South Med J. 1995;8:1212–1216.
88. Hodish I, Ezra D, Gur H, et al. Reiter’s syndrome after intravesical Bacillus Calmette-Guerin therapy for bladder cancer. Isr Med Assoc J. 2000;2:240–241.
89. Hoffler D, Niemever R, Strack G, Zieschang M. Sputum-positive lung tuberculosis after instillation of BCG for bladder. Clin Nephrol. 1991;36:307.
90. Hughes RA, Allard SA, Maini RN. Arthritis associated with adjuvant mycobacterial treatment for carcinoma of the bladder. Ann Rheum Dis. 1989;48:432–434.
91. Iantorno R, Nicolai M, Storto ML, et al. Miliary tuberculosis of the lung in a patient treated with bacillus Calmette-Guerin for superficial bladder cancer. J Urol. 1998;159:1639–1640.
92. Israel-Biet D, Venet A, Sandron D, et al. Pulmonary complications of intravesical Bacille Calmette-Guerin immunotherapy. Am Rev Respir Dis. 1987;135:763–765.
93. Izzes JK, Thomas CB. Corticosteroid-associated fatal mycobacterial sepsis occurring 3 years after instillation of intravesical bacillus Calmette-Guerin. J Urol. 1993;150:1498–1500.
94. Jacob M, Gambrelle J, Fleury J, et al. [Panuveitis following intravesical bacille Calmette-Guerin therapy]. J Fr Ophtalmol. 2006;29:552–555.
95. Jasmer RM, McCowin MJ, Webb WR. Miliary lung disease after intravesical bacillus Calmette-Guerin immunotherapy. Radiology. 1996;201:43–44.
96. Joaquim A, Custodio S, Pimentel FL, et al. Bacillary prostatitis after intravesical immunotherapy: a rare adverse effect. Case Rep Oncol. 2012;5:80–83.
97. Kaklamanos M, Hardavella G, Trigidou R, et al. Multi-organ failure with atypical liver granulomas following intravesical Bacillus Calmette-Guerin instillation. World J Hepatol. 2011;3:79–82.
98. Kamal MM, Soliman SM, Shokeir AA, et al. Bladder carcinoma among live-donor renal transplant recipients: a single-centre experience and a review of the literature. BJU Int. 2008;101:30–35.
99. Kanamori H, Isogami K, Hatakeyama T, et al. Chest wall abscess due to Mycobacterium bovis BCG after intravesical BCG therapy. J Clin Microbiol. 2012;50:533–535.
100. Kanematsu A, Inoue T, Iwamura H, et al. [Intravesical bacillus Calmette-Guerin instillation for patients over 80 years old]. Hinyokika Kiyo. 1998;44:253–257.
101. Katz DS, Wogalter H, D’Esposito RF, et al. Mycobacterium bovis vertebral osteomyelitis and psoas abscess after intravesical BCG therapy for bladder carcinoma. Urology. 1992;40:63–66.
102. Kesten S, Title L, Mullen B, et al. Pulmonary disease following intravesical BCG treatment. Thorax. 1990;45:709–710.
103. Koya MP, Simon MA, Soloway MS. Complications of intravesical therapy for urothelial cancer of the bladder. J Urol. 2006;175:2004–2010.
104. Kristjansson M, Green P, Manning HL, et al. Molecular confirmation of bacillus Calmette-Guerin as the cause of pulmonary infection following urinary tract instillation. Clin Infect Dis. 1993;17:228–230.
105. Kureshi F, Kalaaji AN, Halvorson L, et al. Cutaneous complications of intravesical treatments for bladder cancer: granulomatous inflammation of the penis following BCG therapy and penile gangrene following mitomycin therapy. J Am Acad Dermatol. 2006;55:328–331.
106. Lamm DL, van der Meijden PM, Morales A, et al. Incidence and treatment of complications of bacillus Calmette-Guerin intravesical therapy in superficial bladder cancer. J Urol. 1992;147:596–600.
107. Latini JM, Wang DS, Forgacs P, Bihrle W 3rd. Tuberculosis of the penis after intravesical bacillus Calmette-Guerin treatment. J Urol. 2000;163:1870.
108. Leebeek FW, Ouwendijk RJ, Kolk AH, et al. Granulomatous hepatitis caused by Bacillus Calmette-Guerin (BCG) infection after BCG bladder instillation. Gut. 1996;38:616–618.
109. Leis JA, Ricciuto DR, Gold WL. Attenuated but live: a pelvic abscess caused by bacille Calmette-Guerin. CMAJ. 2011;20:1511–1514.
110. LeMense GP, Strange C. Granulomatous pneumonitis following intravesical BCG. What therapy is needed. Chest. 1994;106:1624–1626.
111. Lester H, Erdey RA, Fastenberg DM, et al. Bacillus Calmette-Guerin (BCG) endophthalmitis. Retina. 1988;8:182–184.
112. Lestre SI, Gameiro CD, João A, Lopes MJ. Granulomas of the penis: a rare complication of intravesical therapy with Bacillus Calmette-Guerin. An Bras Dermatol. 2011;86:759–762.
113. Leyes M, Salas A, Riera M, et al. Pulmonary infiltrates and fever after intravesical instillation of BCG. Ann Med Interna. 1997;14:647–648.
114. Loukil I, Ammari L, Hachicha F. [Unilateral panuveitis following intravesical therapy with bacille of Calmette et Guerin]. Bull Soc Belge Ophtalmol. 2012;320:23–28.
115. Lujan Galan M, Sanchez Sanchez E, Paez Borda A, et al. [Granulomatous prostatitis as a complication of BCG intravesical instillation. Report of a case]. Arch Esp Urol. 1996;49:979–981.
116. Macia Villa C, Sifuentes Giraldo W, Boteanu A, et al. Reactive arthritis after the intravesical instillation of BCG. Reumatol Clin. 2012;8:284–286.
117. Manfredi R, Dentale N, Piergentili B, et al. Tubercular disease caused by Bacillus of Calmette-Guerin as a local adjuvant treatment of relapsing bladder carcinoma. Cancer Biother Radiopharm. 2009;24:621–627.
118. Mangiarotti B, Trinchieri A, Marconato R, Pisani E. Skin abscess after intravesical instillation of bacillus Calmette-Guerin for prophylactic treatment of transitional cell carcinoma. J Urol. 2002;168:1094–1095.
119. Manikandan R, Srirangam S, Vickers D, et al. Penile tuberculosis after intravesical bacillus Calmette-Guerin instillation. BJU Int. 2003;92:e16100.
120. Manzanera Escribano MJ, Morales Ruiz E, Odriozola Grijalba M, et al. Acute renal failure due to interstitial nephritis after intravesical instillation of BCG. Clin Exp Nephrol. 2007;11:238–240.
121. Manzini CU, Bernini L, Elkhaldi N, et al. [Dactilitis and oligoarthritis after BCG immunotherapy in a patient affected by bladder cancer]. Reumatismo. 2006;58:230–232.
122. Marans HY, Bekirov HM. Granulomatous hepatitis following intravesical bacillus Calmette-Guerin therapy for bladder carcinoma. J Urol. 1987;137:111–112.
123. Martinez-Caceres P, Rubio-Briones J, Palou J, et al. Prevesical and inguinal abscess following intravesical BCG instillation. Scand J Urol Nephrol. 1998;32:409–410.
124. Mas AJ, Romera M, Valverde Garcia JM. Articular manifestations after the administration of intravesical BCG. Joint Bone Spine. 2002;69:92–93.
125. Matlaga BR, Veys JA, Thacker C, Assimos DG. Prostate abscess following intravesical bacillus Calmette Guerin treatment. J Urol. 2002;167:251.
126. Maundrell J, Fletcher S, Roberts P, et al. Mycotic aneurysm of the aorta as a complication of Bacillus Calmette-Guerin instillation. J R Coll Physicians Edinb. 2011;41:114–116.
127. Mavrogenis AF, Sakellariou VI, Tsiodras S, Papagelopoulos PJ. Late Mycobacterium bovis spondylitis after intravesical BCG therapy. Joint Bone Spine. 2009;76:296–300.
128. McParland C, Cotton DJ, Gowda KS, et al. Miliary Mycobacterium bovis induced by intravesical bacille Calmette-Guerin immunotherapy. Am Rev Respir Dis. 1992;146:1330–1333.
129. Medina Perez M, Sanchez Gonzalez M, Espino Montoro A. [Hematuria, dysuria, pollakiuria and general malaise, in a patient treated with BCG instillations]. Arch Esp Urol. 1998;51:389–390.
130. Menke JJ, Heins JR. Epididymo-orchitis following intravesical bacillus Calmette-Guerin therapy. Ann Pharmacother. 2000;34:479–482.
131. Michelet N, Spenatto N, Viraben R, et al. [BCG infection of the glans penis after intravesical BCG therapy]. Ann Dermatol Venereol. 2008;135:479–483.
132. Mignon F, Chevriere A, Mesurolle B, et al. [Miliary induced by intravesical BCG immunotherapy for carcinoma of the bladder: CT Findings]. J Radiol. 2002;83:368–371.
133. Milas L, Maillefert JF, Michel F, et al. Refractory arthropathy after intravesical bacillus Calmette-Guerin therapy. Usefulness of isoniazide. Rev Rhum Engl Ed. 1999;66:106–108.
134. Miossec P. Reactive arthritis associated with bacillus Calmette-Guerin immunotherapy for carcinoma of the bladder: immune reactivity against mycobacterial antigens. J Rheumatol. 1996;23:1485.
135. Miranda S, Verdet M, Heron F, et al. [Acute reactive arthritis after intravesical instillation of bacillus Calmette-Guerin. Two case reports and literature review]. Rev Med Interne. 2010;31:558–561.
136. Missioux D, Hermabessiere J, Sauvezie B. Arthritis and iritis after bacillus Calmette-Guerin therapy. J Rheumatol. 1995;22:10.
137. Modesto A, Marty L, Suc JM, et al. Renal complications of intravesical bacillus Calmette-Guerin therapy. Am J Nephrol. 1991;11:501–504.
138. Molina JM, Rabian C, D’Agay MF, Modai J. Hypersensitivity systemic reaction following intravesical bacillus Calmette-Guerin: successful treatment with steroids. J Urol. 1992;147:695–697.
139. Morales A, Eidinger D, Bruce AW. Intracavitary Bacillus Calmette-Guerin in the treatment of superficial bladder tumors. J Urol. 1976;116:180–183.
140. Muttarak M, Lojanapiwat B, Chaiwun B, et al. Preoperative diagnosis of bilateral tuberculous epididymo-orchitis following intravesical Bacillus Calmette-Guerin therapy for superficial bladder carcinoma. Australas Radiol. 2002;46:183–185.
141. Nadasy KA, Patel RS, Emmett M, et al. 2008;101:91–95.
142. Nemeth J, Stoiser B, Winkler HM, et al. Bone marrow infection with bacillus Calmette-Guerin (BCG) after intravesical immunotherapy. Wien Klin Wochenschr. 2008;120:121–123.
143. Nesher G. [Reiter syndrome after intravesical BCG therapy]. Rev Rhum Ed Fr. 1993;60:941.
144. Ng YH, Bramwell SP, Palmer TJ, et al. Cutaneous mycobacterial infection post intravesical BCG installation. Surgeon. 2006;4:57–58.
145. Nikaido T, Ishibashi K, Otani K, et al. Mycobacterium bovis BCG vertebral osteomyelitis after intravesical BCG therapy, diagnosed by PCR-based genomic deletion analysis. J Clin Microbiol. 2007;45:4085–4087.
146. Nunez K, Baldira J, Cuartero M, Roglan A. [Bacillus Calmette-Guerin instillation-induced sepsis]. Med Intensiva. 2010;34:635–636.
147. Obaid S, Weil AG, Rahme R, et al. Mycobacterium bovis spondylodiscitis after intravesical Bacillus Calmette-Guerin therapy. Surg Neurol Int. 2011;2:162.
148. Onur O, Celiker R. Polyarthritis as a complication of intravesical bacillus Calmette-Guerin immunotherapy for bladder cancer. Clin Rheumatol. 1999;18:74–76.
149. Orihuela E, Herr HW, Pinsky CM, Whitmore WF Jr. Toxicity of intravesical BCG and its management in patients with superficial bladder tumors. Cancer. 1987;60:326–333.
150. Ozbakkaloglu B, Tunger O, Surucuoglu S, et al. Granulomatous hepatitis following intravesical bacillus Calmette-Guerin therapy. Int Urol Nephrol. 1999;31:49–53.
151. Palayew M, Briedis D, Libman M, et al. Disseminated infection after intravesical BCG immunotherapy. Detection of organisms in pulmonary tissue. Chest. 1993;104:307–309.
152. Palou J, Angerri O, Segarra J, et al. Intravesical bacillus Calmette-Guerin for the treatment of superficial bladder cancer in renal transplant patients. Transplantation. 2003;76:1514–1516.
153. Pancaldi P, Van Linthoudt D, Alborino D, et al. Reiter’s syndrome after intravesical Bacillus Calmette-Guerin treatment for superficial bladder carcinoma. Br J Rheumatol. 1993;32:1096–1098.
154. Pardalidis NP, Papatsoris AG, Kosmaoglou EV, Georganas C. Two cases of acute polyarthritis secondary to intravesical BCG adjuvant therapy for superficial bladder cancer. Clin Rheumatol. 2002;21:536–537.
155. Paredes Arranz C, de Frutos Arribas JF, Calleja Escudero J, et al. [Miliary dissemination with pulmonary involvement secondary to intravesical immunotherapy with Calmette-Guerin bacillus]. Arch Bronconeumol. 1996;32:100–102.
156. Park CH, Jang MA, Ahn YH, et al. Mycobacterial infection after intravesical bacillus Calmette-Guërin treatment for bladder cancer: a case report. Korean J Lab Med. 2011;31:197–200.
157. Patel AR, Sabanegh ES, Jones JS, et al. Bacillus Calmette-Guerin osteomyelitis mimicking spinal metastasis from urothelial cell carcinoma of the bladder. Eur Urol. 2010;58:934–937.
158. Pittman M, Sakai L, Craig R, et al. Primary aortoenteric fistula following disseminated bacillus Calmette-Guerin infection: a case report. Vascular. 2012;20:221–224.
159. Price GE. Arthritis and iritis after BCG therapy for bladder cancer. J Rheumatol. 1994;21:564–565.
160. Proctor DD, Chopra S, Rubenstein SC, et al. Mycobacteremia and granulomatous hepatitis following initial intravesical bacillus Calmette-Guerin instillation for bladder carcinoma. Am J Gastroenterol. 1993;88:1112–1115.
161. Puett DW, Fuchs HA. Arthritis after bacillus Calmette-Guerin therapy. Ann Intern Med. 1992;117:537.
162. Rabe J, Neff KW, Lehmann KJ, et al. Miliary tuberculosis after intravesical bacille Calmette-Guerin immunotherapy for carcinoma of the bladder. AJR Am J Roentgenol. 1999;172:748–750.
163. Rawls WH, Lamm DL, Lowe BA, et al. Fatal sepsis following intravesical bacillus Calmette-Guerin administration for bladder cancer. J Urol. 1990;144:1328–1330.
164. Reigstad O, Siewers P. A total hip replacement infected with Mycobacterium bovis after intravesicular treatment with Bacille-Calmette-Guerin for bladder cancer. J Bone Joint Surg Br. 2008;90:225–227.
165. Reinert KU, Sybrecht GW. T helper cell alveolitis after bacillus Calmette-Guerin immunotherapy for superficial bladder tumor. J Urol. 1994;151:1634–1637.
166. Reparaz J, Uriz J, Castiello J, Sola J. [Miliary tuberculosis after intravesical Bacillus Calmette-Guerin administration]. Enferm Infecc Microbiol Clin. 1993;11:570.
167. Resel Folkersma LE, Belon Lopez Tomasetti JA, Isorna Martinez de la Riva S, et al. [Complications of endovesical treatment with BCG in a series of 200 patients]. Arch Esp Urol. 1999;52:957–965.
168. Ribera M, Bielsa J, Manterola JM, et al. Mycobacterium bovis-BCG infection of the glans penis: a complication of intravesical administration of bacillus Calmette-Guerin. Br J Dermatol. 1995;132:309–310.
169. Rischmann P, Desgrandchamps F, Malavaud B, et al. BCG intravesical instillations: recommendations for side-effects management. Eur Urol. 2000;37:33–36.
170. Rival G, Garot D, Mercier E, et al. [Acute respiratory failure and septic shock induced by Mycobacterium bovis. A rare side effect of intravesical BCG therapy]. Presse Med. 2006;35:980–982.
171. Rocha MS, Campagnari JC. Granulomatous renal mass after treatment with intravesical bacille Calmette-Guerin. Am J Roentgenol. 1994;163:996–997.
172. Rodriguez F, Palou J, Martinez R, et al. [Practical guideline for the management of adverse events associated with BCG installations]. Arch Esp Urol. 2008;61:591–596.
173. Roylance A, Mosley J, Jameel M, et al. Walker. Aorto-enteric fistula development secondary to mycotic abdominal aortic aneurysm following intravesical bacillus Calmette-Guerin (BCG) treatment for transitional cell carcinoma of the bladder. Int J Surg Case Rep. 2013;4:88–90.
174. Rozenblit A, Wasserman E, Marin ML, et al. Infected aortic aneurysm and vertebral osteomyelitis after intravesical bacillus Calmette-Guerin therapy. Am J Roentgenol. 1996;167:711–713.
175. Safdar N, Abad CL, Kaul DR, et al. Clinical problem-solving. An unintended consequence—a 79-year-old man with a 5-month history of fatigue and 20-lb (9-kg) weight loss presented to his local physician. N Engl J Med. 2008;358:1496–1501.
176. Sakamoto GD, Burden J, Fisher D. Systemic bacillus-Calmette Guerin infection after transurethral administration for superficial bladder carcinoma. J Urol. 1989;142:1073–1074.
177. Salajka F, Pokorny A, Vomela J, et al. Severe complications following intravesical BCG instillation. Monaldi Arch Chest Dis. 2002;57:321–324.
178. Salvador R, Vilana R, Bargallo X, et al. Tuberculous epididymo-orchitis after intravesical BCG therapy for superficial bladder carcinoma: sonographic findings. J Ultrasound Med. 2007;26:671–674.
179. Saporta L, Gumus E, Karadag H, et al. Reiter syndrome following intracavitary BCG administration. Scand J Urol Nephrol. 1997;31:211–212.
180. Satge D, Pommepuy I, Hassan T, et al. Ulcerative terminal ileitis after BCG therapy for bladder carcinoma. Histopathology. 2002;41:266–268.
181. Schellhammer PF, Kaplan MH, Pinsky CM, Whitmore WF Jr. Study of local and systemic effects of intravesical BCG. Urology. 1975;6:562–567.
182. Schellhammer PF. Intravesical bacillus Calmette-Guerin therapy and associated granulomatous renal masses. J Urol. 1987;137:315.
183. Seelig MH, Oldenburg WA, Klingler PJ, et al. Mycotic vascular infections of large arteries with Mycobacterium bovis after intravesical bacillus Calmette-Guerin therapy: case report. J Vasc Surg. 1999;29:377–381.
184. Segal A, Krauss ES. Infected total hip arthroplasty after intravesical bacillus Calmette-Guerin therapy. J Arthroplasty. 2007;22:759–762.
185. Senes AT, Badet L, Lyonnet D, Rouviere O. Granulomatous renal masses following intravesical bacillus Calmette Guerin therapy: the central unaffected calyx sign. Br J Radiol. 2007;80:e230–e233.
186. Shah AN, Uppal GS, Tappin MJ. Bacille Calmette-Guerin and ocular inflammations. Urology. 2011;77:1014–1015.
187. Shakir F, Dang V, Bronze MS, et al. Mycobacterium bovis infection of an axillary-femoral bypass graft after intravesical bacille Calmette-Guerin immunotherapy for bladder cancer. Am J Med Sci. 2009;337:63–64.
188. Sharan S, Thirkill CE, Grigg JR. Autoimmune retinopathy associated with intravesical BCG therapy. Br J Ophthalmol. 2005;89:927–928.
189. Sharma VK, Sethy PK, Dogra PN, et al. Primary tuberculosis of glans penis after intravesical Bacillus Calmette Guerin immunotherapy. Indian J Dermatol Venereol Leprol. 2011;77:47–50.
190. Shelley MD, Court JB, Kynaston H, et al. Intravesical bacillus Calmette-Guerin versus mitomycin C for Ta and T1 bladder cancer. Cochrane Database Syst Rev. 2003;3:CD003231.
191. Shelley MD, Wilt TJ, Court J, et al. Intravesical bacillus Calmette-Guerin is superior to mitomycin C in reducing tumor recurrence in high risk superficial bladder cancer: a meta-analysis of randomized trials. BJU Int. 2004;93:485–490.
192. Sicard D, Steg A, Leleu C, et al. [“BCGitis,” a systemic complication of intravesical BCG therapy of bladder tumor]. Ann Med Interne. 1987;138:555–556.
193. Skoutelis A, Dimitrakopoulos G, Bassaris H. False positive widal reaction in high-titer disseminated BCG infection. Eur J Clin Microbiol Infect Dis. 1994;13:261–263.
194. Smith JA Jr, Labasky RF, Cockett AT, et al. Bladder cancer clinical guidelines panel summary report on the management of nonmuscle invasive bladder cancer (stages Ta, T1 and TIS). The American Urological Association. J Urol. 1999;162:1697–1701.
195. Smith RL, Alexander RF, Aranda CP. Pulmonary granulomata. A complication of intravesical administration of bacillus Calmette-Guerin for superficial bladder carcinoma. Cancer. 1993;71:1846–1847.
196. Somoskovi A, Carlyn C, Dormandy J, Salfinger M. Mediastinal mass mimicking a tumor in a patient with bladder cancer after Bacillus Calmette-Guerin treatment. Eur J Clin Microbiol Infect Dis. 2007;26:937–940.
197. Soylu A, Ince AT, Polat H, et al. Peritoneal tuberculosis and granulomatous hepatitis secondary to treatment of bladder cancer with Bacillus Calmette-Guerin. Ann Clin Microbiol Antimicrob. 2009;8:12.
198. Stanisic TH, Brewer ML, Graham AR. Intravesical bacillus Calmette-Guerin therapy and associated granulomatous renal masses. J Urol. 1986;135:356–358.
199. Steg A, Leleu C, Debre B, et al. Systemic bacillus Calmette-Guerin infection in patients treated by intravesical bacillus Calmette-Guerin therapy for bladder cancer. Eur Urol. 1989;16:161–164.
200. Steg A, Leleu C, Debre B, et al. Systemic bacillus Calmette-Guerin infection in patients treated by intravesical BCG therapy for superficial bladder cancer. Prog Clin Biol Res. 1989;310:325–334.
201. Stone DR, Estes NA 3rd, Klempner MS. Mycobacterium bovis infection of an implantable defibrillator following intravesical therapy with bacille Calmette-Guerin. Clin Infect Dis. 1993;16:825–826.
202. Strock V, Dotevall L, Sandberg T, et al. Late bacille Calmette-Guerin infection with a large focal urinary bladder ulceration as a complication of bladder cancer treatment. BJU Int. 2011;107:1592–1597.
203. Sun HY, Singh N. Should intravesical Bacillus Calmette-Guerin be employed in transplant recipients with bladder carcinoma. Transpl Infect Dis. 2010;12:358–362.
204. Sylvester RJ, Van der Meijden AP, Lamm DL. Intravesical bacillus Calmette-Guerin reduces the risk of progression in patients with superficial bladder cancer: a meta-analysis of the published results of randomized clinical trials. J Urol. 2002;168:1964–1970.
205. Talluri SK, Marigowda L, Besur S, et al. A report of iliac muscle abscess due to Mycobacterium bovis after bacillus Calmette-Guerin therapy for bladder cancer. South Med J. 2010;103:369–370.
206. Tan L, Testa G, Yung T. Diffuse alveolar damage in BCGosis: a rare complication of intravesical bacillus Calmette-Guerin therapy for transitional cell carcinoma. Pathology. 1999;31:55–56.
207. Thepot C, Martigny J, Simon L, et al. Acute polyarthritis after BCG-therapy for bladder carcinoma in a patient with ankylosing spondylitis. Rev Rhum Engl Ed. 1995;62:459–461.
208. Thompson D, Cumming J. Granulomatous hepatitis following intravesical BCG therapy. Br J Urol. 1990;66:432–433.
209. Tillou X, Raynal G, Limani K, et al. [Carcinoma in situ in bladder and urethra among renal transplanted patient: failure of BCG therapy]. Prog Urol. 2008;18:1097–1099.
210. Tinazzi E, Ficarra V, Simeoni S, et al. Reactive arthritis following BCG immunotherapy for urinary bladder carcinoma: a systematic review. Rheumatol Int. 2006;26:481–488.
211. Toscano Rico M, Machado J, Cardoso O, et al. Severe disseminated tuberculosis after intravesical instillation of Bacillus Calmette-Guerin. Eur J Clin Microbiol Infect Dis. 2003;22:447–448.
212. Truelson T, Wishnow KI, Johnson DE. Epididymo-orchitis developing as a late manifestation of intravesical bacillus Calmette-Guerin therapy and masquerading as a primary testicular malignancy: a report of 2 cases. J Urol. 1992;148:1534–1535.
213. Tuncer S, Tekin MI, Ozen H, et al. Detection of bacillus Calmette-Guerin in the blood by the polymerase chain reaction method of treated bladder cancer patients. J Urol. 1997;158:2109–2112.
214. Valentini CG, Bozzoli V, Larici AR, et al. Systemic granulomatous reaction secondary to treatment of bladder cancer with bacillus calmette-guerin. Mediterr J Hematol Infect Dis. 2012;4:e2012040.
215. Van der Meijden AP, Brausi M, Zambon V, et al. Intravesical instillation of epirubicin, bacillus Calmette-Guerin and bacillus Calmette-Guerin plus isoniazid for intermediate and high risk Ta, T1 papillary carcinoma of the bladder: a European Organization for Research and Treatment of Cancer genito-urinary group randomized phase III trial. J Urol. 2001;166:476–481.
216. Van der Meijden AP, Steerenberg PA, van Hoogstraaten IM, et al. Immune reactions in patients with superficial bladder cancer after intradermal and intravesical treatment with bacillus Calmette-Guerin. Cancer Immunol Immunother. 1989;28:287–295.
217. Van der Meijden AP. Practical approaches to the prevention and treatment of adverse reactions to BCG. Eur Urol. 1995;27((suppl 1)):23–28.
218. Van Outryve SM, Francque SM, Gentens PA, et al. Bacillus Calmette-Guerin-induced granulomatous hepatitis in a patient with a superficial bladder carcinoma. Eur J Gastroenterol Hepatol. 2004;16:1027–1032.
219. Van Thiel RJ, Nossent GD, Tjon Pian Gi NP. Articular complications of intravesical BCG treatment for bladder carcinoma. Br J Urol. 1992;70:446–447.
220. Vazquez-Lavista LG, Flores-Balcazar CH, Llorente L. [The bacillus Calmette-Guerin as immunomodulator in bladder cancer]. Rev Invest Clin. 2007;59:146–152.
221. Viallard JF, Denis D, Texier-Maugein J, et al. Disseminated infection after bacille Calmette-Guerin instillation for treatment of bladder carcinoma. Clin Infect Dis. 1999;29:451–452.
222. Villamil-Cajoto I, Jove MJ, Serrano M, et al. [Granulomatous hepatitis due to Mycobacterium complex following Bacillus Calmette-Guerin intravesical instillation]. Enferm Infecc Microbiol Clin. 2010;28:759–761.
223. Wada Y, Sugiyama Y, Kikukawa H, et al. Isolated renal tuberculosis following intravesical Bacillus Calmette-Guerin therapy for bladder cancer. Urol Int. 2004;72:257–260.
224. Wang HB, Hsieh HH, Chen YT, et al. The outcome of post-transplant transitional cell carcinoma in 10 renal transplant recipients. Clin Transplant. 2002;16:410–413.
225. Wertheim M, Astbury N. Bilateral uveitis after intravesical BCG immunotherapy for bladder carcinoma. Br J Ophthalmol. 2002;86:701–707.
226. Witjes JA, Vriesema JL, Brinkman K, et al. Mycotic aneurysm of the popliteal artery as a complication of intravesical BCG therapy for superficial bladder cancer. Case report and literature review. Urol Int. 2003;71:430–432.
227. Wolf YG, Wolf DG, Higginbottom PA, Dilley RB. Infection of a ruptured aortic aneurysm and an aortic graft with bacille Calmette-Guerin after intravesical administration for bladder cancer. J Vasc Surg. 1995;22:80–84.
228. Yates J, Stein B. Bladder and penile lesions with inguinal adenopathy after intravesical Bacillus Calmette-Guerin (BCG) treatment. Urology. 2007;70:e15–e17.
229. Yoshida R, Kawasaki H, Miyajima A, et al. Primary tuberculosis of the penis after intravesical bacillus Calmette-Guerin instillation therapy. J Eur Acad Dermatol Venereol. 2009;23:77–78.
230. Yossepowitch O, Eggener S, Bochner B, et al. Safety and efficacy of intravesical bacillus Calmette-Guerin Instillations in steroid treated and immunocompromised patients. J Urol. 2006;176:482–485.
231. Youssef M, Carre P, Asquier E, et al. Miliary pulmonary tuberculosis following intravesical BCG-therapy. Rev Pneumol Clin. 2003;59:201–204.
232. Yusuke H, Yoshinori H, Kenichi M, et al. Granulomatous balanoposthitis after intravesical Bacillus-Calmette-Guerin instillation therapy. Int J Urol. 2006;13:1361–1363.
233. Zabraniecki L, Negrier I, Vergne P, et al. Fluoroquinolone induced tendinopathy: report of 6 cases. J Rheumatol. 1996;23:516–520.