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Pretreatment With Pro- and Synbiotics Reduces Peritonitis-Induced Acute Lung Injury in Rats

Tok, Demet MD; Ilkgul, Ozer MD; Bengmark, Stig MD, PhD; Aydede, Hasan MD; Erhan, Yamac MD; Taneli, Fatma MD; Ulman, Cevval MD; Vatansever, Seda MD; Kose, Can MD; Ok, Gulay MD

Journal of Trauma and Acute Care Surgery: April 2007 - Volume 62 - Issue 4 - p 880-885
doi: 10.1097/01.ta.0000236019.00650.00
Original Articles

Background: To study whether enteral pretreatment with a synbiotic composition of lactic acid bacteria and bioactive fibers can reduce peritonitis-induced lung neutrophil infiltration and tissue injury in rats.

Materials and Methods: Rats were divided into five groups, and subjected to induction of peritonitis-induced lung injury using a cecal ligation and puncture model (CLP). All animals were pretreated for 3 weeks prior the CLP by daily gavage with either (1) a synbiotic composition (1010 CFU of Pediococcus pentosaceus 5-33:3, 1010 CFU of Leuconostoc mesenteroides 77:1, 1010 CFU of L. paracasei subspecies paracasei, 1010 CFU of L. plantarum 2362 plus fermentable fibers), (2) fermentable fibers alone, (3) nonfermentable fibers, (4) a probiotic composition (1010 CFU of P. pentosaceus 5-33:3, 1010 CFU of L. mesenteroides 77:1, 1010 CFU of L. paracasei subsp. paracasei, 1010 CFU of L. plantarum 2,362), or (5) a heat-killed probiotic composition. All animals were killed 24 hours after CLP and lung tissue samples were studied for degree of neutrophil infiltration and levels of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β. In addition the lung wet-to-dry tissue weight ratio, the myeloperoxidase activity, and malondialdehyde content were also assessed.

Results: No mortality was encountered in any of the groups. Histologic signs of lung injury (number of neutrophils and TNF-α, IL-1β staining) were observed in all groups except the synbiotic and probiotic treated groups. Myeloperoxidase activity and malondialdehyde content were significantly lower in the two lactobacillus- pretreated groups, with no difference between them. Heavy infiltration of lung tissue with neutrophils was observed only in fiber-treated (302.20 ± 7.92) and placebo-treated (266.90 ± 8.92) animals. This was totally abolished in the synbiotic-treated group (34.40 ± 2.49). Lung edema (wet-to-dry lung weight ratio) was significantly reduced in the synbiotic-treated group (4.92 ± 0.13 vs. 5.07 ± 0.08 and 5.39 ± 0.10, respectively).

Conclusion: Three weeks of preoperative enteral administration of a synbiotic composition reduced peritonitis-induced acute lung injury in rats in a CLP model.

© 2007 Lippincott Williams & Wilkins, Inc.