We hypothesized that by studying a suspension of a single cell type in various resuscitation fluids
, we could compare their effects on parameters associated with cell death.
Methods Jurkat cells
were suspended in resuscitation fluids
. Using flow cytometry, light scatter, phosphatidylserine translocation, propidium iodide uptake, and intracellular H2
production were measured.
Buffer (pH, 7.4) and albumin
added to Ringer’s lactate inhibited the adverse changes, including intracellular oxygen free radical production. Oxygen free radical production was variable within the cell population and inhibited by albumin
but not other colloids or crystalloids. This correlated well with the ability of albumin
to enhance metabolic activity. A flavoprotein inhibitor blocked H2
production, suggesting that mitochondria are the source of the H2
and the variability.
Oxygen free radical inhibition by albumin
could explain both its beneficial and its harmful effects.