Background
Unexpected immunomodulatory effects of colloids and crystalloids prompted an investigation of
albumin’s ability to prevent bone marrow (BM) suppression following
trauma/
hemorrhagic shock (T/HS: laparotomy + MAP 30 for 90 mins).
Methods
In vitro: Normal rat BM was plated for granulocyte-macrophage (CFU-GM) and erythrocyte colony forming units (BFU-E) with 2% v/v plasma from sham (T/SS) or T/HS rats and
albumin (2–8 mg/mL). In vivo: Male rats (n = 4/group) were subjected to T/SS or T/HS and resuscitated with shed blood and twice the volume as Lactated Ringer’s (LR) or blood and 1, 2, or 3 mL of
albumin (50 mg/mL). Bone marrow harvested 3 hours post-resuscitation was plated for CFU-GM and BFU-E.
Results
In vitro: T/HS plasma decreased both CFU-GM and BFU-E growth as compared with T/SS, whereas increasing doses of
albumin showed dose-dependent improvement in progenitor growth (
p < 0.05). In vivo: The suppression of BM red and white cell progenitor growth seen in T/HS+LR rats as compared with T/SS was fully prevented by as little as 1 mL of
albumin (
p < 0.05).
Conclusions
Small doses of
albumin fully restore CFU-GM and BFU-E to sham values. We postulate that the binding of circulating toxic factors by
albumin may play a role in this prevention of T/HS-induced BM suppression.
