Unexpected immunomodulatory effects of colloids and crystalloids prompted an investigation of albumin
’s ability to prevent bone marrow (BM) suppression following trauma
(T/HS: laparotomy + MAP 30 for 90 mins).
In vitro: Normal rat BM was plated for granulocyte-macrophage (CFU-GM) and erythrocyte colony forming units (BFU-E) with 2% v/v plasma from sham (T/SS) or T/HS rats and albumin
(2–8 mg/mL). In vivo: Male rats (n = 4/group) were subjected to T/SS or T/HS and resuscitated with shed blood and twice the volume as Lactated Ringer’s (LR) or blood and 1, 2, or 3 mL of albumin
(50 mg/mL). Bone marrow harvested 3 hours post-resuscitation was plated for CFU-GM and BFU-E.
In vitro: T/HS plasma decreased both CFU-GM and BFU-E growth as compared with T/SS, whereas increasing doses of albumin
showed dose-dependent improvement in progenitor growth (p
< 0.05). In vivo: The suppression of BM red and white cell progenitor growth seen in T/HS+LR rats as compared with T/SS was fully prevented by as little as 1 mL of albumin
Small doses of albumin
fully restore CFU-GM and BFU-E to sham values. We postulate that the binding of circulating toxic factors by albumin
may play a role in this prevention of T/HS-induced BM suppression.