Gene transfer is a new territory for clinicians. Intractable disorders might be approached in such a way. Adeno-associated virus (AAV) vector has been transfected successfully into a variety of tissues including skin. We evaluated the ability of this vector to transfer and cause expression of the lacZ reporter gene in human keloid tissue.
Human keloid specimens were injected with an AAV vector encoding Escherichia coli β-galactosidase and incubated for 4 weeks after injection. The presence of lacZ mRNA and β-galactosidase enzymatic activity were assayed by reverse-transcriptase polymerase chain reaction and the X-gal technique.
Gene expression shown by reverse-transcriptase polymerase chain reaction was observed in keloid tissue 4 weeks after injection, and so was the positive X-gal staining.
Our results showed that AAV vector could transduce human keloid tissue effectively. Replacement of the reporter gene with a functioning gene might be feasible for keloid treatment.
From the School of Medicine, National Yang-Ming University (H.M., R.-H.F., H.C., H.-S.K.), Division of Plastic Surgery (H.M., R.-H.F.) and Department of Neurosurgery (H.C., H.-S.K.), Veterans General Hospital–Taipei, Taipei, Taiwan, Institute of Molecular Biology, The University of Hong-Kong (R.X.), Hong Kong, and CNS Gene Therapy Center, Jefferson Medical College and Thomas Jefferson University (M.D.), Philadelphia, Pennsylvania.
Submitted for publication November 13, 2001.
Accepted for publication September 16, 2002.
Supported, in part, by the Veterans General Hospital-Taipei (grant 88-285) and the National Science Council, Taiwan, Republic of China (grant NSC-89-2314-B-075-120).
Address for reprints: Hsu Ma, MD, PhD, Division of Plastic Surgery, Veterans General Hospital–Taipei, 201 Shih-Pai Road, Sec. 2, Taipei 11217, Taiwan, Republic of China; email: firstname.lastname@example.org.