The purpose of the study was to determine whether electrolyzed oxidized water (EOW) functions as a bactericide in burn injury with Pseudomonasaeruginosa infection in a rat burn-wound model.
Anesthetized Sprague-Dawley rats (n = 31) were subjected to third-degree burns to 30% of total body surface area. Two days after injury, all rats were infected with P. aeruginosa using 1 mL of a suspension containing 1 × 108 colony-forming units. Rats were assigned to one of three groups: no irrigation (group I), irrigation with physiologic saline (group II), or irrigation with EOW (group III). Blood culture, endotoxin levels, and survival rates were determined.
Survival rate was significantly higher in group III than in groups I or II (p < 0.0001). Serum endotoxin levels on day 3 after infection in group III were significantly lower than the levels in group I (p < 0.01) and group II (p < 0.01). There were significant differences between the three groups in the culture of P. aeruginosa (p < 0.05).
Irrigation and disinfection with EOW may become useful in preventing burn-wound sepsis.
From the Department of Emergency and Critical Care Medicine, Akita University School of Medicine, 1–1-1 Hondo, Akita 010-8543, Japan.
Submitted for publication October 5, 1999.
Accepted for publication March 6, 2000.
Address for reprints: Hajime Nakae, MD, Department of Emergency and Critical Care Medicine, Akita University School of Medicine, 1–1-1 Hondo, Akita 010-8543, Japan. Fax: 018-884-6450.
Supported, in part, by Grant 10470311 from the Ministry of Education, Science, and Culture of Japan.