MISCELLANEOUSPrevention of growth arrest by fibrin interposition into physeal injuryJie, Qianga; Hu, Yunyua; Yang, Liua b; Lei, Weia; Zhao, Lic; Lv, Ronga; Wang, JunaAuthor Information aDepartment of Orthopaedic Surgery, Xi-jing Hospital, Xi'an bDepartment of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong cDepartment of Pediatric Orthopaedics and Rehabilitation, Xin-Hua Hospital, Shanghai Jiao Tong University, Shanghai, China Correspondence to Professor Yunyu Hu, PhD, Department of Orthopaedic Surgery, Xijing Hospital, Xi'an, Shaanxi 710032, China Tel: +860 29 84771012; fax: +860 29 83244986; e-mail: [email protected] Journal of Pediatric Orthopaedics B: March 2010 - Volume 19 - Issue 2 - p 201-206 doi: 10.1097/BPB.0b013e3283327fdb Buy Metrics Abstract This study investigated the repair effects of fat and fibrin graft interposition through a proximal tibia transphyseal injury model and assessed the effectiveness of treatment to physeal injury with the fibrin. In this study, a unilateral growth plate injury was created in the right proximal tibia of 28 rats without any graft interposition; all left tibias were left untouched. In the other group of 28 rats, a bilateral physeal injury was made with the left tibia filled with autogenously adipose tissue and the right tibia filled with fibrin. To compare the malformed extents induced by different interventions, the length and the metaphyseal–diaphyseal angle of the tibia of three injured groups were examined. Further studies on bone density analysis and histological change were used to compare the bony bridge formation under different interventions. Results showed that the deformity angle and medial length of the tibia were significantly different between the grafted groups and nongrafted group at 4, 16, and 24 weeks postoperative (P<0.01). Results also showed no significant difference between fibrin-graft and fat-graft groups (P>0.05). Furthermore, the bone mineralization density of bony bridge induced by injury was significantly different between the grafted group and nongrafted group at 4, 16, and 24 weeks postoperative (P<0.01). Histological findings showed that bony repair after physeal injury was inhibited by both fibrin and fat interventions. We concluded that fibrin could be a substitute of adipose tissue in preventing the deformities induced by epiphyseal injury. Similar to autogenous fat, fibrin was found to alleviate limb shortness and prevent angular malformation by forming a scar instead of a bony bridge. The use of fibrin can help us to develop effective and compound intervention grafts to prevent skeletal deformity and regenerate normal cartilage tissue in the future. © 2010 Lippincott Williams & Wilkins, Inc.