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Investigation of Dynamic Thiol/Disulfide Homeostasis in Children With Acute Immune Thrombocytopenia

Beyazit, Husam BSc*; Demiryürek, Abdullah T. PhD*; Temel, Münevver T. MD; Pekpak, Esra MD; Demiryürek, Seniz PhD§; Akbayram, Sinan MD

Journal of Pediatric Hematology/Oncology: August 2019 - Volume 41 - Issue 6 - p 463–467
doi: 10.1097/MPH.0000000000001494
Original Articles
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Oxidative stress may play a role in the pathogenesis of immune thrombocytopenia (ITP), but the role of dynamic thiol/disulfide homeostasis has not been studied. The objective of this study was to assess whether there is a change in thiol/disulfide homeostasis in children with acute ITP. A total of 40 children with acute ITP and 50 healthy age-matched and sex-matched controls were included in this study. Serum total thiol and native thiol levels have been measured with a novel automatic spectrophotometric method. The amount of dynamic disulfide bonds and related ratios were calculated from these values. The average total thiol and native thiol levels of the patient group were found to be significantly lower than those levels of controls (P<0.01). However, intravenous immunoglobulin (IVIG) treatment with 1 g/kg/d prevented these reductions. disulfide level was slightly, but not significantly, depressed in ITP patients, but it recovered following IVIG treatment. We detected no marked changes in disulfide/total thiol, disulfide/native thiol, and native thiol/total thiol ratios between groups. These results are the first to demonstrate that thiol/disulfide homeostasis plays a role in ITP pathogenesis, and IVIG treatment can prevent the reduced thiol levels in children.

Departments of *Medical Pharmacology

Pediatrics

§Physiology, Faculty of Medicine

Department of Pediatrics, Division of Hematology and Oncology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey

Supported by a research project (TF.YLT.17.26) from the University of Gaziantep.

The authors declare no conflict of interest.

Reprints: Abdullah T. Demiryürek, PhD, Department of Medical Pharmacology, Faculty of Medicine, University of Gaziantep, Gaziantep 27310, Turkey (e-mail: demiryurek@gantep.edu.tr).

Received July 27, 2018

Accepted March 21, 2019

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