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The human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) DNA repair enzyme and its association with lung cancer risk

Park, Jonga; Chen, Lana; Tockman, Melvyn Sa,b; Elahi, Abula; Lazarus, Philipa

ORIGINAL ARTICLES
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Objective The human 8-oxoguanine DNA N-glycosylase 1 gene encodes a DNA glycosylase that is involved in the base excision repair of 8-hydroxy-2-deoxyguanine from oxidatively-damaged DNA and expressed in lung tissue. The codon 326 polymorphism in the hOGG1 gene has been suggested to reduce DNA repair enzyme activity based on in vitro functional analysis. The goal of the present study is to determine whether the codon 326 polymorphism was significantly associated with alterations in individual risk for lung cancer.

Methods To determine whether hOGG1 plays a role in risk for lung cancer, we measured the prevalence of the Ser326Cys polymorphism in incident lung cancer patients and matched non-cancer controls. hOGG1 genotyping was performed by PCR-restriction fragment length polymorphism analysis of genomic DNA isolated from 179 Caucasian lung cancer cases and 358 controls individually matched in a 1:2 ratio by race-, sex- and age (± 5 years).

Results Significantly increased risk for lung cancer was observed for both the hOGG1 326Ser/Cys (odds ratio [OR] = 1.9, 95% confidence interval [CI] = 1.2–2.9) and hOGG1 326Cys/Cys genotypes (OR = 3.8, 95% CI = 1.4–10.6). The increased risk for lung cancer was observed for subjects with both the hOGG1 326Ser/Cys (OR = 1.7, 95% CI = 1.1–2.8) and hOGG1 326Cys/Cys genotypes (OR = 4.9, 95% CI = 1.5–16.1) in ever-smokers. A significant association was found between hOGG1 genotypes and lung cancer risk with a dose–dependent effect with smoking. Significantly increased risk for variant hOGG1 genotypes was observed for all non-small cell lung cancer patients.

Conclusion These results suggest that the hOGG1 Ser326Cys polymorphism plays an important role in the risk for lung cancer and is linked to exposure to tobacco smoke.

aDivisions of Cancer Control and Molecular Oncology, H. Lee Moffitt Cancer Center, University of South Florida, Tampa, FL, USA and Department of Interdisciplinary Oncology, University of South Florida, Tampa, FL, USA and bMolecular Screening Program, H. Lee Moffitt Cancer Center, University of South Florida, Tampa, FL, USA.

These studies were supported in part by R03-CA9131 (JP), U01-CA08497 (MT) U01-CA08497 supplement (JP), DAMD17-01-2-0056 (the Advanced Cancer Detection Center grant from the US Army Medical Research) (JP), P01-CA68384 (PL) and R01-DE13158 (PL). The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.

Correspondence to Jong Park, Ph.D., H. Lee Moffitt Cancer Center, University of South Florida, OSWFBB, 12902 Magnolia Drive, Tampa, FL 33612, USA. Tel: +1 813 632-1703; fax: +1 813 632-1720; e-mail: parkj@moffitt.usf.edu

Received 5 September 2003 Accepted 4 November 2003

© 2004 Lippincott Williams & Wilkins, Inc.