Original ArticlesRole of alcohol and genetic polymorphisms of CYP2E1 and ALDH2 in breast cancer developmentChoi, Ji-Yeoba; Abel, Josefb; Neuhaus, Thomasc; Ko, Yonc; Harth, Volkerc; Hamajima, Nobuyukid; Tajima, Kazuod; Yoo, Keun-Younga; Park, Sue Kyunge; Noh, Dong-Youngf; Han, Wonshikf; Choe, Kuk-Jinf; Ahn, Sei-Hyung; Kim, Sook-Una; Hirvonen, Arih; Kang, Daeheea,iAuthor Information aDepartment of Preventive Medicine, fGeneral Surgery and iCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea; bDepartment of Experimental Toxicology, Research Institute of Environmental Health at the Heinrich-Heine-University of Düsseldorf, Düsseldorf, cDepartment of Internal Medicine, University of Bonn, Bonn, Germany; dDivision of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Japan; eDepartment of Preventive Medicine, Konkuk University College of Medicine, Chungju, Korea; gDepartment of General Surgery, Ulsan University College of Medicine, Seoul, Korea and hDepartment of Industrial Hygiene and Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland. Correspondence to Daehee Kang, Department of Preventive Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong Chongno-Gu, Seoul 110–799 Korea. Tel: +82 2 740 8326; fax: +82 2 747 4830; e-mail: [email protected] Received 28 June 2002 Accepted 18 November 2002 Pharmacogenetics: February 2003 - Volume 13 - Issue 2 - p 67-72 Buy Abstract Objective We examined the potential association between alcohol consumption and genetic polymorphisms in the alcohol metabolizing enzymes, CYP2E1 and ALDH2, in individual susceptibility to breast cancer in a Korean study population. Methods Three hundred and forty-six histologically confirmed breast cancer patients and 377 controls with no present or previous history of cancer were recruited from several teaching hospitals in Seoul during 1995–2001. The CYP2E1 RsaI polymorphism was determined by a real time PCR method, and the ALDH2 Glu487Lys polymorphism was determined by a PCR method with confronting two-pair primers (PCR-CTPP). Results The drinking women had a 1.4-fold risk for breast cancer (95% CI = 0.99–2.11) compared to never drinkers after adjustment for age and family history of breast cancer. No statistically significant overall differences were seen in the genotype frequencies between breast cancer cases and controls. However, the ‘ever'-drinking women with the CYP2E1 c2 allele containing genotypes had a 1.9-fold risk (95% CI = 0.99–3.83) for developing breast cancer compared to non-drinkers with the CYP2E1 c1/c1 genotype (P for interaction = 0.043). Conclusion This study therefore suggests that the CYP2E1 c2 allele may influence the individual susceptibility to breast cancer in alcohol-consuming women. © 2003 Lippincott Williams & Wilkins, Inc.