Effect of Oral Lipid Matrix Supplement on Fat Absorption in Cystic Fibrosis: A Randomized Placebo-Controlled Trial : Journal of Pediatric Gastroenterology and Nutrition

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Original Articles: Pancreatology

Effect of Oral Lipid Matrix Supplement on Fat Absorption in Cystic Fibrosis: A Randomized Placebo-Controlled Trial

Stallings, Virginia A.; Schall, Joan I.; Maqbool, Asim; Mascarenhas, Maria R.; Alshaikh, Belal N.; Dougherty, Kelly A.; Hommel, Kevin; Ryan, Jamie; Elci, Okan U.; Shaw, Walter A.

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Journal of Pediatric Gastroenterology and Nutrition 63(6):p 676-680, December 2016. | DOI: 10.1097/MPG.0000000000001213


Pancreatic enzyme therapy does not normalize dietary fat absorption in patients with cystic fibrosis and pancreatic insufficiency. Efficacy of LYM-X-SORB (LXS), an easily absorbable lipid matrix that enhances fat absorption, was evaluated in a 12-month randomized, double-blinded, placebo-controlled trial with plasma fatty acids (FA) and coefficient of fat absorption (CFA) outcomes. A total of 110 subjects (age 10.4 ± 3.0 years) were randomized. Total FA increased with LXS at 3 and 12 months (+1.58, +1.14 mmol/L) and not with placebo (P = 0.046). With LXS, linoleic acid (LA) increased at 3 and 12 months (+298, +175 nmol/mL, P 0.046), with a 6% increase in CFA (P< 0.01). LA increase was significant in LXS versus placebo (445 vs 42 nmol/mL, P= 0.038). Increased FA and LA predicted increased body mass index Z scores. In summary, the LXS treatment improved dietary fat absorption compared with placebo as indicated by plasma FA and LA and was associated with better growth status.

What Is Known

  • Chronic fat malabsorption and loss of dietary calories persist in children and adults with cystic fibrosis and pancreatic insufficiency in spite of optimization of pancreatic enzyme replacement therapy and diet.

What Is New

  • A randomized double blind clinical trial showed that supplementation with an easily absorbed structured lipid improved dietary fat absorption and fatty acid status, as indicated by increase in total fatty acid and linoleic acid concentrations and improved stool coefficient of fat absorption.
  • Improved total fatty acid and linoleic acid concentrations were associated with improved growth status in children.

Nutritional status remains suboptimal in patients with cystic fibrosis (CF) and pancreatic insufficiency (PI) despite efforts to optimize pancreatic enzymes and diets (1–3). Chronic steatorrhea results in loss of fat calories and fat soluble vitamins (4–7), and stool coefficient of fat absorption (CFA) often ranges from 81% to 85% (8–10). In a recent study of >200 patients, those with CFA <85% had growth faltering (6). Essential fatty acid (FA) insufficiency has been associated with poorer growth and pulmonary function, and is in part a consequence of chronic fat malabsorption (9,11–17). Second generation LYM-X-SORB (LXS) (BioMolecular Products, Byfield, MA; Avanti Polar Lipids, Alabaster, AL) (18) with enhanced taste and mixability is a phospholipid- and triglyceride-rich matrix. The aim of this randomized placebo-controlled double-blinded trial was to evaluate the efficacy of 12-month supplementation with LXS to improve fat absorption indicated by plasma FA concentrations and stool CFA.


Subjects ages 5.0 to 17.9 years with CF, PI, and mild-to-moderate lung disease were recruited from 10 CF centers. LXS is composed of lysophosphatidylcholine, triglycerides, and FA, which form an organized lipid matrix complexed to wheat flour and sugar. Subjects ages 5.0 to 11.9 years received 2 packets/day, and subjects ages 12.0 to 17.9 years received 3 packets/day. The placebo had similar calories (∼150 kcal/packet), total fat, FA, and macronutrient distribution. The inclusion/exclusion criteria, study design and method details, LXS composition, and the results for LXS impact on choline status have been previously reported (10,19,20).

Dietary intake was assessed using 3-day weighed records (21,22). A 72-hour stool sample was collected, total fat content determined (Mayo Medical Laboratories, Rochester, MN), and CFA calculated (8). Daily pancreatic enzyme medication use was determined. Subjects completed the Pediatric Quality of Life Inventory (PedsQL 4.0) (23) and the Cystic Fibrosis Questionnaire-Revised (CFQ-R) (24,25).

Quantitation of morning fasting plasma FA was performed in 2 steps: acid-base hydrolysis and hexane extraction/derivatization with pentafluorobenzyl bromide (Mayo Medical Laboratories). Separation/detection was accomplished by capillary gas chromatography electron-capture negative ion-mass spectrometry, with quantitation based on analysis in selected ion monitoring mode using 13 stable isotope-labeled internal standards (26).

Descriptive statistics were presented as frequency counts and percentages for categorical variables and mean ± standard deviation for continuous variables. Two-sample t tests (unpaired) or Mann–Whitney U test for continuous variables and χ2 tests of independence for categorical variables compared characteristics at baseline. Paired t tests were used to determine the significance of change in quality-of-life scores from baseline to 12 months.

Changes in outcome measures over time, the main effect of randomization groups, and randomization group (R) by time (T) interactions were investigated on an intent-to-treat basis using mixed effects linear regression models implemented via maximum likelihood accounting for correlations arising from the repeated measures (27). Whether changes in outcomes over time differed by randomization groups were evaluated by examining the interaction effects of randomization groups by time (R × T). Similar secondary analysis limited to subjects with linoleic acid (LA) in the lower quartile range at baseline was performed. Group comparison of percent change in FA during 3 and 12 months was analyzed using Wilcoxon rank-sum tests. Exploratory analyses using regression models assessed associations among baseline plasma total FA or LA, change (Δ) in total FA or LA status, and change in clinical outcomes over time, adjusted for age, sex, and adherence to supplement use. Stata version 12.1 (Stata Corporation, College Station, TX) was used with significance at 0.05.


There were 110 subjects recruited, 56 randomized to placebo and 54 to LXS supplementation. Subjects (10.4 ± 3.0 years) were 57% male, 57% ΔF508 homozygous, and had mild pulmonary disease (forced expiratory volume at 1 second 95 ± 23% predicted), suboptimal growth status (height-for-age Z score −0.4 ± 0.9, weight-for-age Z score [WAZ] −0.4 ± 0.8, body-mass-index-for-age Z score [BMIZ] −0.2 ± 0.8), and moderate fat malabsorption (CFA 83 ± 12%). The overall PedsQL was 82 ± 12 for parent-assessed and 81 ± 13 for the child-assessed measures. The randomization groups did not differ at baseline for any measure.

A total of 24 subjects after baseline (10 placebo and 14 LXS) and 16 subjects after 3 months (7 placebo and 9 LXS) withdrew from the study. Neither the attrition rate nor the reasons for dropping out differed by randomization group (28) (Supplementary Fig. 1, https://links.lww.com/MPG/A653). Dropouts and study completers did not differ at baseline in age, sex, genotype, growth status, plasma FA, intake of dietary fat, LA, or pancreatic enzyme use.

Table 1 presents dietary intake of fat, pancreatic enzyme use, fat absorption, and FA for placebo and LXS groups. There were no differences between randomization groups at baseline. The dietary intake included both food intake and supplement (LXS or placebo) adjusted for adherence. Energy intake averaged 119% of the estimated energy requirement and 36% of fat calories. Refer to Supplementary Table 1 (https://links.lww.com/MPG/A653) for the expanded list of dietary intake and plasma FA variables. LXS and placebo supplements provided 300 to 450 calories/day depending on age. Enzyme medication use was less in LXS than placebo group at 12 months (P = 0.007). Intake of LA was similar in both groups throughout the study (Supplementary Table 1, https://links.lww.com/MPG/A653). CFA improved significantly (6%) in LXS only at both 3 and 12 months (P < 0.01). For the LXS group, 39% had moderate fat malabsorption (CFA < 80%) at baseline and declined to 11% at 3 months (P = 0.013) and 15% at 12 months (P = 0.025), with no change in the placebo group. Cumulative adherence to supplements was 80% versus 76% at 3 months, and 75% versus 71% at 12 months for placebo versus LXS, respectively.

Table 1:
Dietary intake, stool fat absorption, plasma FAs by placebo, and LXS at baseline, 3 and 12 months

LXS supplementation was associated with increased absorption of total FA during 3 and 12 months (R × T, P0–3–12 = 0.046), and monounsaturated fatty acid (MUFA, R × T, P0–3–12= 0.043), and of saturated fatty acid (SFA) and MUFA during 3 months (R × T, P0–3 < 0.022) compared with placebo with the intent-to-treat approach (Supplementary Table 1, https://links.lww.com/MPG/A653). Increase from baseline in fasting plasma total FA, SFA, MUFA, polyunsaturated fatty acid (PUFA), and LA was robust (11%–18%) with LXS compared with small increases (2%–6%) for placebo (Supplementary Fig. 2, https://links.lww.com/MPG/A653). Palmitic acid (most abundant SFA), and oleic acid (most abundant MUFA) were significantly increased in LXS compared with placebo. LA (the most abundant PUFA) significantly increased within the LXS group only at 3 and 12 months (P< 0.05); the difference from placebo approached significance at 3 months (R × T, P0–3 = 0.069). The baseline mean triene:tetraene ratio (T:T ratio) was 0.05 ± 0.02 in both groups. Elevated T:T ratio >0.05, a cutoff used to indicate essential FA deficiency using non-CF-specific laboratory reference ranges (26), was documented in 41% at baseline (39% and 43% in placebo and LXS, respectively) and was 26% after 12 months of supplementation (23% and 29% placebo and LXS, respectively). This decline was significant for the groups combined (1-sided Fisher exact test, P = 0.025).

The impact of change in total FA (Δ total FA, increase or decrease) and LA status (Δ LA) on clinical outcomes was explored in the LXS and placebo groups combined. Adjusted for baseline status, age, sex, and adherence, Δ total FA positively predicted BMIZ (ß coefficient = 0.04, P = 0.011) and fat-free mass (FFM) (ß = 0.19, P = 0.005), whereas Δ LA positively predicted WAZ (ß = 0.10, P = 0.042) and BMIZ (ß = 0.07, P = 0.003) (Supplementary Table 2, https://links.lww.com/MPG/A653). Improved weight and body mass index (BMI) status was largely attributable to increases in FFM.

Change in LA in subjects whose baseline LA was in the lowest quartile range for this sample (≤1920 μmol/L) was analyzed (10 in placebo and 17 in LXS group). These subjects had lower BMI at baseline compared with the other quartiles combined (−0.48 ± 0.82 vs −0.11 ± 0.73, P= 0.03). LA increased significantly in the LXS group from baseline to 3 and 12 months (1640 ± 198 to 1936 ± 392 and 2120 ± 580 nmol/mL, respectively, P ≤ 0.002). No significant change in LA occurred in the placebo group (1693 ± 184 to 1806 ± 429 and 1752 ± 332 nmol/mL, respectively). LA change over time between LXS and placebo was significant at 12 months (R × T, P0–12 = 0.038).

We have previously reported that both LXS and placebo were safe (10,28), and growth status improved in both groups (10,20). The quality-of-life score indicated by the PedsQL or CFQ-R did not change, and there were no differences by randomization group. When the groups were combined, there were significant increases from baseline to 12 months in child-assessed emotional functioning subscales from both the PedsQL (75.7 ± 18.5 to 80.8 ± 16.2, P = 0.01 by paired t test) and the CFQ-R (76.8 ± 15.3 to 81.2 ± 14.4, P = 0.01), and social functioning and perceived body image (67.0 ± 18.4 to 72.1 ± 16.6 and 77.4 ± 26.0 to 85.3 ± 22.2, respectively, P < 0.01) on the CFQ-R.


LXS was developed to improve dietary fat absorption when combined with foods and beverages. In children with CF and PI, daily oral supplementation with this easily absorbable phospholipid- and triglyceride-rich lipid matrix was safe and effective, as indicated by increased fasting plasma FA and 6% increase in stool CFA. Absorption improved with LXS in SFA, MUFA, and PUFA compared with placebo, and in LA in the LXS group only, despite similar energy and fat intake in both groups and fewer pancreatic enzymes in the LXS group. Improvement in total FA and LA concentrations was associated with better weight and BMI status. Participants had dietary patterns that closely adhered to CF recommendations, with high calorie intake (119%–124% estimated energy requirement), high fat intake of 97 to 106 g/day (34%–36% kcal from fat), and appropriate doses of pancreatic enzyme medication for meals and snacks (1–4). CFA at baseline was 82% in this cohort of diet- and enzyme-adherent subjects, compared with the reference value of 93% dietary fat absorption in healthy people (5,29).

Chase et al (30) were among the first to provide oral LA and follow the fasting plasma FA absorption response. McKenna et al (31) then used both naturally occurring and structured triglycerides to identify fat sources that would enhance absorption in CF and PI. They concluded that both long-term adequate calories and supplemental LA were necessary to prevent essential FA deficiency, with optimized pancreatic enzyme replacement. A number of studies to improve LA status with different designs and different LA sources have been conducted (31–36), none of which offered LA in a more readily absorbable form. In our CF study, baseline dietary intake of LA was 159% of the recommendation for healthy people (∼18 g/day). Both LXS and placebo supplements contained a small amount of LA, and daily intake increased about 15% (to 21 g) in both groups. Only the LXS group showed a significant increase in the fasting plasma LA, particularly among those with the lowest baseline LA concentrations. These findings indicate that the unique LXS lipid structure and composition resulted in improved LA absorption efficiency.

The more frequently described FA abnormalities in CF include low LA, docosahexaenoic acid (DHA), PUFA, and high T:T ratios (13–15). Few subjects in the present study had baseline LA concentrations below laboratory reference range. More than one third, however, had essential FA insufficiency based upon the laboratory reference range for the T:T ratio. It is notable, however, that for subjects more at risk for LA insufficiency at baseline (LA ≤1920 μmol/L), LA concentrations increased by 29% during 12 months in LXS with no change in the placebo group. The proportion of subjects with essential FA insufficiency based upon the T:T ratio declined significantly from 41% at baseline to 26% after 12 months, with no group difference. This was expected for the LXS group with improved absorption of multiple FAs, but the reason for this improvement in the placebo group was less clear. Perhaps the additional dietary energy, FA and LA in either LXS or placebo supported change in this ratio. It should be noted that this ratio method was developed before reliable actual measurement of plasma LA was clinically available to detect this specific essential FA deficiency.

LA deficiency has clinical consequences including impaired growth, wound healing, and seborrheic dermatitis (37). In addition to PI and fat malabsorption (5,38), plasma FA abnormalities may relate to increased energy expenditure, increased oxidative stress (39,40), increased cell membrane turnover (41), abnormal release of FA from the cell membrane (42), and defects related to specific CF genotypes (12,43). Suboptimal FA status has been associated with poorer pulmonary and growth outcomes in CF (13,44,45). The type and amount of dietary fat intake predicts the FA status including plasma LA, T:T ratio, and DHA status in CF (9,46). Here, LXS improved overall fat absorption, and total FA and LA status improvement was associated with increased WAZ and BMIZ, likely attributable to increased FFM relative to FM. Children with the lowest LA concentrations at baseline had significantly lower BMIZ. This may indicate that LXS has the potential to impact growth and nutritional status in children with CF at risk for essential FA insufficiency. Health-related quality of life improved in the domains of emotional functioning, social functioning, and body image, suggesting that the improved weight status observed in both randomization groups was psychosocially beneficial, though this requires further examination.

Study strengths included a study design that supports generalizability with enrollment from 10 CF centers, successful randomization, and good adherence. Limitations include subject attrition that may have introduced bias; however, there was no evidence that dropouts and completers differed at baseline in demographic, clinical outcome measures, or plasma FA status. Reasons for dropping out did not differ by randomization groups. The study design did not include either a CF or a non-CF nonintervention group for comparison. The dose of LXS was based on a prior study with LXS in which increased FA concentrations were observed. A higher dose may have a different effect on the outcomes of interest.

In summary, LXS treatment improved dietary fat absorption compared with placebo as indicated by plasma FA, LA, and CFA in children with CF and PI who had similar intake of energy, fats, and LA, and less pancreatic enzyme medication use. Increased total FA and LA absorption may support better growth status in school-aged children. Based on these results and clinical experience, LXS has the potential to reduce fat malabsorption in CF clinical care. Furthermore, investigation is needed to evaluate the effectiveness of LXS to reduce fat malabsorption and treat or prevent malnutrition in patients with other diagnoses.


The authors thank the subjects, parents, other care providers, and all of the CF centers that participated in the study: Children's National Medical Center, Washington, DC; Children's Hospital of Philadelphia, Philadelphia, PA; Monmouth Medical Center, Long Branch, NJ; The Pediatric Lung Center, Fairfax, VA; Cystic Fibrosis Center of University of Virginia, Charlottesville, VA; Children's Hospital of the King's Daughters, Eastern Virginia Medical School, Norfolk, VA; Yale University School of Medicine, New Haven, CT; Cohen Children's Medical Center, New Hyde Park, NY; St Joseph's Children's Hospital, Paterson, NJ, and the Pediatric Specialty Center at Lehigh Valley Hospital, Bethlehem, PA. The authors also thank Norma Latham, MS, for study coordination, and Megan Johnson, Thananya Wooden, Elizabeth Matarrese, and Nimanee Harris, and the staff of the Clinical Translational Research Center at Children's Hospital of Philadelphia for their valuable contributions to the study.


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fatty acids; linoleic acid; LYM-X-SORB; pancreatic insufficiency

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