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Original Article: Gastroenterology

Treatment of Acute Diarrhea With Saccharomyces boulardii in Infants

Corrêa, Naflesia B.O.; Penna, Francisco J.*; Lima, Fátima M.L.S.; Nicoli, Jacques R.; Filho, Luciano A.P.*

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Journal of Pediatric Gastroenterology and Nutrition: November 2011 - Volume 53 - Issue 5 - p 497-501
doi: 10.1097/MPG.0b013e31822b7ab0
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Acute gastroenteritis is the second most common life-threatening condition worldwide among all of the infectious diseases in children younger than 5 years, and the first cause of hospitalization (1). A variety of viral, bacterial, and parasitic organisms have been implicated in the pathogenesis of acute infectious diarrhea, and about 50% to 80% of episodes are caused by viruses, mainly rotaviruses and noroviruses. The management of acute diarrhea consists of the replacement of lost fluid with oral rehydration solution containing glucose and electrolytes; however, this solution does not reduce the severity and the duration of diarrhea. The search for a treatment allowing such reduction started decades ago, and probiotics have been proposed as complementary therapy to the use of rehydration solution in the treatment of acute diarrhea (2).

According to the presently adopted definition by the Food and Agriculture Organization/World Health Organization, probiotics are “live microorganisms, which when administered in adequate amounts confer a health benefit to the host” (3). Several preparations containing different microorganisms have been tested in clinical trials and/or are commercially available. Meta-analyses of probiotic efficacy for acute diarrhea are available (4–7), and even though all of them concluded that probiotics have moderate clinical benefits in the treatment of acute diarrhea in children, they also considered that the results should be interpreted with caution because of the methodological limitations of the studies and that more research is needed. The European Society for Pediatric Gastroenterology, Hepatology, and Nutrition together with the European Society for Pediatric Infectious Diseases have concluded that probiotics may be used as an adjunct in the management of acute diarrhea and recommend 2 of them in particular, Lactobacillus rhamnosus GG and Saccharomyces boulardii, because of a higher volume of convincing data (8).

S boulardii is a nonpathogenic yeast that has been used successfully for the prevention and/or the treatment of antibiotic-associated diarrhea, acute gastroenteritis in adults and children, and Clostridium difficile–associated disease (9,10). In the case of infectious diarrhea, administration of S boulardii provides protection against intestinal lesions caused by several diarrheal pathogens (11). Five single- or double-blind controlled clinical trials using S boulardii as a probiotic have been conducted for the treatment of acute diarrhea in children (12–16). Four among the 5 trials with the yeast showed it to be beneficial in children admitted to the hospital for diarrhea. In the fifth trial, which compared 5 different probiotic preparations, S boulardii had no clinical effect. To explain these contradictory results, the authors of this last study suggest that the clinical conditions of the children could be responsible for the difference (more severe condition in almost all of the positive studies and mild to moderate diarrhea in the fifth trial).

There is a general consensus in the scientific literature that more clinical trials using probiotics are warranted because of numerous unanswered questions such as the efficiency of the treatment of infectious diarrhea depending on the etiologic agent. The present double-blind, placebo-controlled trial was performed to evaluate the efficacy of a commercial product containing S boulardii for the treatment of acute diarrhea in hospitalized children.


Trial Design

The trial was conducted using a double-blind, randomized, placebo-controlled, parallel-group design from April 2007 to September 2008. The study was approved by the ethical council of the medical school, Universidade Federal de Goiás, and was registered as a clinical trial with the protocol number CEPMHA/HC/UFG 147/06. Before enrollment, the purpose of the study and the procedures were explained in detail, and written consent was obtained from the parents.


Inclusion criteria were children of both sexes and ages 6 to 48 months, no other diarrhea episode or antibiotics use 2 weeks before the trial, and acute diarrhea within 72 hours before hospitalization. Exclusion criteria included exclusive breast-feeding, presence of macroscopic blood in the feces, severe malnutrition (weight/height <70% National Center for Health Statistics), impossibility of oral nutrition, existence of underlying pathology (eg, sepsis, cystic fibrosis, renal insufficiency, liver disease), and children needing specific treatment. The energy requirement for each infant was calculated and updated by a pediatric nutritionist who daily evaluated all of the patients admitted to the hospital. Parameters evaluated for eligibility of the patients were diarrhea duration before the intervention, dehydration state, number of evacuations 24 hours before the intervention, and stool consistency (liquid, semiliquid, soft, and formed). Diarrhea was defined as a change in bowel habits with a diminution of stool consistency and 3 or more evacuations per day with a duration of not more than 72 hours.

Study Settings

Children hospitalized for acute diarrhea were screened at 1 of the 2 following hospitals: Hospital da Criança and Hospital Infantil de Campinas, both located in Goiânia, Goiás, Brazil.


S boulardii was provided as capsules, which were purchased from a local pharmaceutical establishment (Floratil; Merck S.A., Rio de Janeiro, Brazil). The capsules contained 200 mg of the lyophilized yeast (4 × 109 viable cells) and magnesium stearate, lactose, and sucrose as excipients. Placebo capsules contained 200 mg of only the excipients and were prepared by the Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, Brazil. In the probiotic group, the patients were treated orally every 12 hours with the capsule containing the yeast for 5 days. In the control group, patients received placebo following the same schedule as the probiotic group.


The endpoint was clinical cure of the diarrhea, which was considered to have occurred when evacuation frequency was <3 times per day or the stool consistency improved for at least 24 hours. During the trial period, if no improvement was observed in 4 days of intervention, then the therapy was discontinued and child was remanded for further treatment of diarrhea.

Sample Size

The EpiInfo statistical package (version 6.04, Centers for Disease Control and Prevention, Atlanta, GA) was used to determine the sample size that would provide 80% power using a 2-sided test (with α = 5%) for differences in proportions, based on an expected frequency of diarrhea with a duration higher than 3 days of 55% for the placebo group and 30% for the probiotic group. Under these assumptions, the smallest sample size was 68 patients in each group, totaling 136 children. Taking into account a study withdrawal rate of 25%, the target sample size was increased to 170 patients.


The capsules were randomly coded by computer-generated numbers and distributed to the attending staff, which was composed of 2 physicians, 2 nurses, and 2 nutritionists.


Both placebo and lyophilized S boulardii were packaged in identical capsules. Powders in both types of capsules were similar in texture and color, and the attending staff was unaware which product was being administered.

Additional Analyses

The presence of rotavirus was detected in fecal samples from 162 patients using commercially available enzyme-linked immunosorbent assay kits (Oxoid, Basingstoke, UK).

Statistical Methods

Data were analyzed using EpiInfo, and P < 0.05 or P < 0.01 were considered statistically significant depending on the data analyzed. Differences between group proportions were assessed using the Yates continuity-corrected χ2 test. Analysis was performed both as per protocol and as intention to treat.


Figure 1 shows that a total of 186 children with acute diarrhea fulfilled the protocol inclusion and exclusion criteria and were randomized in the probiotic (95) and placebo groups (91). In our trial, 3 patients from the probiotic group and 2 from the placebo group were excluded from analysis during the trial because of the need for antibiotic treatment, and 2 patients from the probiotic group and 3 from the placebo group were excluded because of withdrawal during the trial. By the end, 176 children completed the trial: 90 in the probiotic group and 86 in the placebo group. No adverse effects were noticed in the probiotic or placebo group that required discontinuation of the interventions.

CONSORT diagram.

Table 1 summarizes the subjects’ baseline, demographic, and clinical characteristics. The 2 groups were comparable with regard to age, sex, and baseline features of acute diarrhea. Rotavirus also was detected in similar frequency in the 2 groups, with an average of 57.4% of the children with diarrhea tested for the presence of the virus.

Baseline characteristics by study group

Figure 2 shows that 2 days after the beginning of the intervention, the frequency of patients who remained with diarrhea was lower in the probiotic group when compared with the placebo group (P < 0.01). The difference between the 2 groups was maintained by day 3 of intervention, with 32.2% of patients with diarrhea in the group treated with S boulardii and 59.2% in the placebo group (P < 0.01).

Frequency (%) of infants with diarrhea during the first 3 days after the beginning of the intervention with S boulardii (▪) and the placebo (□) as analyzed per protocol. *Statistically different values (P < 0.01).

Intention to treat analysis (Table 2) shows that the frequency of patients with diarrhea 3 days after the beginning of treatment was lower in the group treated with S boulardii when compared with the placebo group, which represents a statistically significant reduction in risk when the probiotic was used (relative risk [RR] 0.54, 95% confidence interval [CI] 0.38–0.66, P < 0.001). Per-protocol analysis showed similar results (RR 0.54, 95% CI 0.38–0.77, P < 0.001).

Frequency of diarrhea 3 days after beginning of intervention with S boulardii or placebo for patients, analyzed as per protocol and as intention to treat

Table 3 shows that in an exploratory analysis, when the children pertaining to the 2 types of intervention were separated into rotavirus-infected patients and nonrotavirus patients, the beneficial effect resulting from probiotic treatment was observed essentially for patients presenting with rotaviral diarrhea, with 29.2% of children who remained with diarrhea in the group treated with S boulardii and 64.4% in the placebo group (RR 0.45, 95% CI 0.28–0.74). In patients with nonrotaviral diarrhea, 41.2% of children treated with S boulardii remained with diarrhea, whereas in the placebo group, this frequency was 54.3% (RR 0.76, 95% CI 0.46–1.26); however, this difference was not confirmed as being significant when the ratio of the 2 relative risks tested as statistically different from 1 (ratio 0.60; P = 0.15).

Frequency of diarrhea 3 days after beginning of intervention with S boulardii or placebo for patients presenting or not rotavirus in groups in which presence of rotavirus was evaluated (n = 162)


Although oral rehydration solution remains the mainstay in the treatment of acute diarrhea, this therapy does not reduce the duration of diarrhea, prompting a growing interest in adjunctive treatments. The present study demonstrated that oral treatment with S boulardii diminished in approximately 50% of the patients with diarrhea since the second day after the beginning of the intervention when compared with a placebo group. The timing of the first administration of the probiotic appears to be critical, because the earlier the first administration of S boulardii, the greater the efficacy. Vilarruel et al (14) showed that children given S boulardii within 48 hours of the onset of diarrhea had significantly fewer number of stools than those who were administrated the product when the duration of diarrhea was >48 hours. This was the reason for the selection of patients with acute diarrhea within 72 hours before hospitalization as 1 of the inclusion criteria in the present study.

Diarrhea is a multifaceted disease with respect to etiology, and some intervention may be more or less effective with diarrhea caused by specific pathogens. To explore this possibility, the children were separated into 2 groups in each intervention type: rotavirus-infected patients and nonrotavirus patients. The mean frequency of patients with diarrhea caused by rotavirus in both intervention groups (57.4%) was slightly higher than the range from 16% to 52% that was observed in other studies in Latin America (17). The frequency of patients with diarrhea was lower in the rotaviral group (29.2%) than in the nonrotaviral group (41.2%), 3 days after the beginning of the intervention with the probiotic, but this difference was not found to be significant when the relative risks in the 2 groups were compared statistically. Several controlled trials have shown that selected strains of lactobacilli (meanly L rhamnosus GG) exhibited both therapeutic and prophylactic effects in children with viral but not bacterial diarrhea (18–20). In addition, Bifidobacterium bifidum combined with Streptococcus thermophilus has shown prophylactic activity against rotavirus gastroenteritis (21); however, an inverse result was obtained by Sarker et al (22) with Lactobacillus paracasei strain ST11, which had no effect on rotavirus but ameliorated the outcome of nonrotaviral diarrhea in children from Bangladesh. To explain this discrepancy with the results obtained in the previous studies, the authors suggested that the difference could be related to the severity of the illness. In previously published studies, children were affected less severely than those in the study by Sarker et al (22). Curiously, this is the same argument used by Canani et al (15) to explain the lack of protective action of S boulardii against acute diarrhea in children in their study when compared with 4 other clinical trials, but with an inverse finality. Interestingly, clinical evaluation and biological experiments in animal models showed that for Lactobacillus GG the protective effect against rotavirus was dependent on probiotic viability (23).

Concerning the mode of action of the probiotic, it is probable that >1 mechanism is involved when viruses are the pathogenic agents. Probiotics are well-known modulators of the mucosal immune response, stimulating its beneficial and/or suppressing deleterious aspects. Stimulation of the production of secretory immunoglobulin A and of reactive oxygen species (NO and H2O2) by intestinal epithelial cells when treated with probiotic could explain a protective effect against virus (23–25). Secretory immunoglobulin A serves as the first line of defense against many mucosal pathogens, and in the case of rotavirus, protection against the infection appears to rely mainly on the production of neutralizing antibodies against the outer capsid proteins VP4 and VP7 (26). Another explanation could be the anti-inflammatory effect of S boulardii resulting from yeast secretory protein(s), which inhibit(s) proinflammatory cytokines by interfering with the global mediator of inflammation nuclear factor-κB and modulating the activity of the mitogen-activated protein kinases ERK1/2 and p38 (27). Competition for attachment site onto epithelial intestinal cells between virus and probiotic could be another possibility as demonstrated in a vesicular stomatitis virus cell line infection (28). Complementary mechanisms of bacterium and yeast probiotic action are the trapping of pathogenic virus and bacteria by fixation onto its cell surface (28,29) and the production of metabolic compounds with antiviral activity as demonstrated for some lactic acid–producing bacteria against vesicular stomatitis virus (28). Finally, stimulation of the activity of brush border disaccharidases (lactase, sucrase) could be an additional mechanism to explain the antidiarrheal activity of the yeast (24).

Results from this clinical trial support the use of S boulardii as an adjunctive treatment in acute diarrhea, based on a reduction of diarrhea duration when S boulardii was given to children within 72 hours after the onset of acute diarrhea. The results also suggest a better apparent efficiency when rotavirus was the etiological agent, but this needs to be confirmed by larger specific follow-up studies.


1. Weizman Z. Probiotics use in childhood acute diarrhea. A web-based survey. J Clin Gastroenterol 2011; 45:426–428.
2. Elmer GW, Surawicz CM, McFarland LV. Biotherapeutic agents: a neglected modality for the treatment and prevention of selected intestinal and vaginal infections. JAMA 1996; 275:870–876.
3. Food and Agriculture Organization of the United Nations and World Health Organization. Guidelines for the Evaluation of Probiotics in Food, Food and Agriculture. Organization of the United Nations & World Health Organization Working Group Report, London, Ontario, Canada, April 30–May 1, 2002.
4. Huang JS, Bousvaros A, Lee JW, et al. Efficacy of probiotic use in acute diarrhea in children. Dig Dis Sci 2002; 47:2625–2634.
5. Sazawal S. Efficacy of probiotics in prevention of acute diarrhea: a meta-analysis of masked, randomized, placebo-controlled trials. Lancet Infect Dis 2006; 6:374–382.
6. Szajewska H, Skórka A, Dylag M. Meta-analysis: Saccharomyces boulardii for treating acute diarrhoea in children. Aliment Pharmacol Ther 2007; 25:257–264.
7. Szajewska H, Skórka A, Ruszczynski M, et al. Meta-analysis: Lactobacillus GG for treating acute diarrhoea in children. Aliment Pharmacol Ther 2007; 25:871–881.
8. Guarino A, Albano F, Ashkenazi S, et al. European Society for Pediatric Gastroenterology, Hepatology and Nutrition/European Society for Paediatric Infectious Diseases evidence-based guidelines for the management of acute gastroenterititis in children in Europe: executive summary. J Pediatr Gastroenterol Nutr 2008; 46:619–621.
9. Vandenplas Y, Brunser O, Szajewska H. Saccharomyces boulardii in childhood. Eur J Pediatr 2009; 168:253–265.
10. MacFarland LV. Systematic review and meta-analysis of Saccharomyces boulardii in adult patients. World J Gastroenterol 2010; 16:2202–2222.
11. Czerucka D, Piche T, Rampal P. Review article: yeast as probiotics: Saccharomyces boulardii on diarrheal pathogens. Aliment Pharmacol Therap 2007; 26:767–778.
12. Cetina-Sauri G, Busto GS. Therapeutic evaluation of Saccharomyces boulardii in children with acute diarrhea. Ann Pediatr 1994; 6:397–400.
13. Kurugol Z, Koturoglu G. Effects of Saccharomyces boulardii in children with acute diarrhea. Acta Paediatr 2005; 94:44–47.
14. Villarruel G, Rubio DM, Lopez F, et al. Saccharomyces boulardii in acute childhood diarrhoea: a randomized, placebo-controlled study. Acta Paediatr 2007; 96:538–541.
15. Canani RB, Cirillo P, Terrin GA, et al. Probiotics for treatment of acute diarrhoea in children: randomized clinical trial of five different preparations. BMJ 2008; 335:340–347.
16. Grandy G, Medina M, Soria R, et al. Probiotics in the treatment of acute rotavirus diarrhoea. A randomized, double-blind, controlled trial using two different probiotic preparations in Bolivian children. BMC Infect Dis 2010;10:253.
17. Kane M, Turcios R, Arvay M, et al. The epidemiology of rotavirus diarrhea in Latin America anticipating rotavirus vaccine. Rev Panam Salud Publica 2004; 16:371–377.
18. Oberhelman RA, Gilman RH, Sheen P, et al. A placebo-controlled trial of Lactobacillus GG to prevent diarrhea in undernourished Peruvian children. J Pediatr 1999; 134:15–20.
19. Guandalini S, Pensabene L, Zikri MA, et al. Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea: a multicenter European trial. J Pediatr Gastroenterol Nutr 2000; 30:54–60.
20. Szajewska H, Kotowska M, Mrukowicz JZ, et al. Efficacy of Lactobacillus GG in prevention of nosocomial diarrhea in infants. J Pediatr 2001; 138:361–365.
21. Saavedra JM, Bauman NA, Oung I, et al. Feeding of Bifidobacterium bifidum and Streptococcus thermophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet 1994; 344:1046–1049.
22. Sarker SA, Sultana S, Fuchs GJ, et al. Lactobacillus paracasei strain ST11 has no effect on rotavirus but ameliorates the outcome of non-rotavirus diarrhea in children from Bangladesh. Pediatrics 2005; 116:e221–e228.
23. Kaila M, Isolauri E, Saxelin M, et al. Viable versus inactivated Lactobacillus strain GG in acute rotavirus diarrhea. Arch Dis Child 1995; 72:51–53.
24. Buts JP, de Keyser N. Effects of Saccharomyces boulardii on intestinal mucosa. Dig Dis Sci 2007; 51:1485–1492.
25. Maragkoudakis PA, Chingwaru W, Gradisnik L, et al. Lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection. Intern J Food Microbiol 2010; 141:S91–S97.
26. Ruggeri FM, Johansen K, Basile G, et al. Anti-rotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice against diarrhea. J Virol 1998; 72:2708–2714.
27. Pothoulakis C. Review article: anti-inflammatory mechanisms of action of Saccharomyces boulardii. Aliment Pharmacol Ther 2009; 30:826–833.
28. Botic T, Klinberg TB, Weingartl H, et al. A novel eukaryotic cell culture model to study antiviral activity of potential probiotic bacteria. Int J Food Microbiol 2007; 115:227–234.
29. Martins FS, Dalmasso G, Arantes MRE, et al. Interaction of Saccharomyces boulardii with Salmonella enterica serovar typhimurium protects mice and modifies T84 cell response to the infection. PLoS ONE 2010; 5:e8925.

acute diarrhea; clinical trial; probiotic; rotavirus; Saccharomyces boulardii

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