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Original Articles: Hepatology & Nutrition

Biliary Atresia: Swiss National Study, 1994–2004

Wildhaber, Barbara E*; Majno, Pietro*; Mayr, Johannes; Zachariou, Zacharias; Hohlfeld, Judith§; Schwoebel, Marcus||; Kistler, Walter; Meuli, Martin#; Le Coultre, Claude*; Mentha, Gilles*; Belli, Dominique*; Chardot, Christophe*

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Journal of Pediatric Gastroenterology and Nutrition: March 2008 - Volume 46 - Issue 3 - p 299-307
doi: 10.1097/MPG.0b013e3181633562
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Abstract

Biliary atresia (BA) is a rare neonatal disease of unknown etiology and is characterized by obstruction of the biliary tree, causing severe cholestasis and biliary cirrhosis, that leads finally to death in the first years of life. The recommended treatment of BA is sequential: In the first weeks of life, the Kasai portoenterostomy (PE), or its technical variants, aim to restore the biliary flow to the intestine; in the case of failure of the operation and/or life-threatening complications of the biliary cirrhosis, liver transplantation (LT) may eventually be needed. The reported incidence of BA in several European countries varies between 1 in 14,000 and 1 in 20,000 live births (1–5); however, the epidemiology of BA in Switzerland is unknown.

The outcome of patients with BA has dramatically improved over the last several decades. After the worldwide diffusion of the Kasai operation in the late 1960s (6) and the development of pediatric LT in the 1980s, the reported survival rates of patients with BA in industrialized countries today reach 90% (7). In survivors, the ratio of transplanted patients varies, depending on the results of the Kasai operation and the duration of follow-up. Until now, the outcome of BA in Switzerland has never been studied. Hence, from the analysis of all of the children with BA born in Switzerland between 1994 and 2004, the aim of this study was to describe the epidemiology of BA in Switzerland, to study the outcome of these children from diagnosis, and to analyze the prognostic factors influencing this outcome.

PATIENTS AND METHODS

The records of all patients born in Switzerland between January 1994 and December 2004 and diagnosed with BA were reviewed. All 7 pediatric surgery centers (Basel, Berne, Geneva, Lausanne, Lucerne, St Gallen, and Zurich) involved in the management of patients with BA contributed to the study. Centers were visited and charts were analyzed by a single investigator (B.E.W.) to reassess the diagnosis of BA and to achieve homogenous data collection.

Inclusion Criteria

The criteria for inclusion of patients were as follows:

  1. Patients with BA diagnosed by means of clinical, biochemical, radiological, histological, and surgical findings, all consistent with BA (8). Histology of the biliary remnant was available for all of the patients, including those without previous Kasai PE.
  2. Patients born, living, and treated primarily in Switzerland between January 1994 and December 2004. The Kasai PE or its modifications, as well as LT, were available for every child during this period, the medical costs being covered by the national medicosocial insurance system.

Analysis of Records

Recorded data included birth date; clinical and biochemical data of the period pre-, peri-, and post-Kasai PE; surgical and histological findings; whether or not LT was performed; clinical and biochemical data of the period pre-, peri- and post-LT; final outcome; date of last follow-up; cause of death; and possible reasons why a Kasai PE or LT were not performed. Macroscopic classification of BA was based on the anatomical pattern of the extrahepatic biliary remnant according to the French classification (8): type 1 (BA limited to the common bile duct), type 2 (biliary cyst in the liver hilum, communicating with dystrophic intrahepatic bile ducts), type 3 (gallbladder, cystic duct and common bile duct patent), and type 4 (complete extrahepatic BA). Success of the Kasai PE was defined as complete clearance of jaundice and a total bilirubin level of ≤20 μmol/L. An intermediate result of the Kasai PE was defined as a minimal bilirubin level after the Kasai between 20 and 50 μmol/L. A failed Kasai PE was defined when bilirubin level did not decrease below 50 μmol/L. Median follow up was 58 months, with a range of 5 to 124 months. Swiss demographic data were obtained from the Swiss Federal Statistic Office.

Ethical Approval

The present study was approved by the ethical committee of the University of Geneva, Switzerland. Each family was contacted and informed of this study and gave its written consent.

Statistical Analysis

Epidemiology

Incidences were expressed as observed value and calculated confidence interval of 95% (95% CI). egional variations in incidence were compared by the χ2 test, with Yates correction if indicated. Annual and seasonal variations in incidences were analyzed by comparing cumulated observed frequencies by months and years, and expected frequencies according to variations of births, assuming a constant incidence of BA (expected frequency of BA = constant incidence × observed live births during the considered period of time). The comparison used the χ2 test, with Yates correction if indicated.

Outcome

Survival rates were calculated using the Kaplan-Meier method: survival with native liver (SNL) (starting at birth, endpoint being LT or death), survival after LT (starting at birth, endpoint being death), and overall patient survival (OS) (starting at birth, endpoint being death). Survival was expressed in percentage ± standard error. Potential prognostic factors were analyzed by the log rank test. Categorical data used the χ2 test, with Yates correction if indicated. Statistical significance was defined as P < 0.05.

RESULTS

Epidemiology

BA was diagnosed in 48 children (33 girls, 15 boys) born in Switzerland between 1994 and 2004. Incidence was 1 in 17,800 live births (95% CI 1/13,900–1/24,800). No significant regional variation in incidence was found (Fig. 1). No significant annual and monthly variation in incidence was observed.

FIG. 1
FIG. 1:
Incidence of biliary atresia patients born between 1994 and 2004 in Switzerland: overall Swiss incidence 1/17,800 live births (95% CI 1/13,900–1/24,800), Swiss population = 7.4 million. Regional distribution of incidences was as follows: (1) western, French-speaking 1/14,200 live births (95% CI 1/9500–1/27,800), (2) northern, German-speaking 1/20,500 live births (95% CI 1/15,000–1/32,500), (3) southeastern, Italian- and Romanch-speaking (Ticino and Grisons) 1/13,400 (95% CI 1,6800–1/670,000) (P = NS). No significant regional variation in incidence was found.

Patients and Analysis of Medical History

Median birth weight was 3315 g, range 1410 to 4000 g, median gestational age was 39 weeks, range 32 to 42 weeks. Observation of first clinical signs was made in 63% of subjects by the pediatrician, and in 31% of subjects by the mother. Four in 48 children (8%) presented with polysplenia syndrome (syndromatic BA); 6 in 48 children (13%) had other associated anomalies or diseases, including uropathies (ureteropelvic junction obstruction, vesicouretral reflux, megaureter), Kartagener syndrome, and glycogenosis type IX.

Kasai PE

Forty-three children underwent a Kasai PE. The Kasai PE was performed in 5 different pediatric surgery units: Berne (6 cases), Geneva (21 cases), Lausanne (1 case), Lucerne (1 case), and Zurich (14 cases). The median age at Kasai operation was 68 days (range, 30–126 days). All but 3 patients underwent a classic Kasai PE, without stoma or antireflux valve; 3 patients had an extended Kasai PE according to the technique described by Schweizer et al (9). The biliary remnant was type 4 in 41 of 48 (85%) patients, type 3 in 4 patients, type 2 in 2 patients, and type 1 in 1 patient. No standardized postoperative medical regimen (steroids, ursodeoxycholic acid, antibiotics) was used.

Clearance of Jaundice Post-Kasai

After the Kasai operation, bilirubin levels normalized (≤20 μmol/L) in 17 of 43 (39.5%) patients, decreased between 20 and 50 μmol/L in 5 of 43 (11.6%) patients, and remained above 50 μmol/L in 21 of 43 (48.8%) patients. Fourteen of 17 patients whose bilirubin levels returned to normal values did not need LT. Two of 5 patients whose bilirubin levels decreased between 20 and 50 μmol/L underwent LT. All 21 patients whose bilirubin levels remained above 50 μmol/L received transplants or died while waiting for an LT.

The postoperative evolution after Kasai PE is represented in Figure 2. Results of the general outcome of patients with BA are summarized in Figure 3.

FIG. 2
FIG. 2:
Evolution of 43 patients with biliary atresia who underwent Kasai portoenterostomy. LT = liver transplantation.*Before 2001; **anicteric bilirubin level ≤20 μmol/L.
FIG. 3
FIG. 3:
Results of general outcome of Swiss patients with BA, born between 1994 and 2004: 91.7% of all patients were alive at the end of the study.

Survival With Native Liver

Survival with native liver (SNL) (Fig. 4) of all patients with BA from diagnosis was 40.5% ± 7.4% at 2 years and 32.7% ± 7.2% at 4 and 5 years. Survival with native liver in patients who underwent Kasai PE was 43.4% ± 7.9% at 2 years and 37.4% ± 7.9% at 4 and 5 years. Survival with native liver after the Kasai operation was strongly linked to the clearance of jaundice after the Kasai operation: 2- and 5-year SNL was 92.3% ± 7.4% and 83.9% ± 10.4% in patients with successful Kasai operation, 60% ± 21.9% and 60% ± 21.9% in patients with intermediate results of the Kasai operation, and 4.8% ± 4.6% and 0% in patients with failed Kasai operation, the longest SNL being 28 months in this group of patients (P < 0.0001).

FIG. 4
FIG. 4:
Overall survival of patients with BA and SNL from diagnosis.

LT and Survival After LT

Liver transplantation was needed in 31 of 48 children with BA, including 5 patients without previous Kasai PE (4 of those 5 patients were born before 1997, so no Kasai PE was performed because these patients were considered to be too old for the operation). Four patients died while waiting for LT, all before 2001, all of them after failed Kasai PE. Thus, 26 in 43 patients (60.4%) needed LT after the Kasai procedure. In total, 29 LTs were performed in 27 patients, including 2 re-LTs. The types of liver grafts are shown in Table 1. All of the patients received transplant operations in Geneva, except 1 in Paris (Kremlin-Bicêtre, France). Liver transplantation was performed at a median age of 11.7 months (range, 5.3–66.7 months). With a median follow up of 60 months (range, 5–132 months), all of the patients are alive after LT.

TABLE 1
TABLE 1:
Type of liver graft

Overall Patient Survival

Overall 2-, 4- and 5-year BA patients survival (Fig. 4) was 91.5% ± 4.1%. Two- and 5-year OS of patients after successful Kasai or intermediate result of the Kasai operation was 100%. In patients with failed Kasai operation, 2- and 5-year OS was 81% ± 8.6%. All 5 patients who did not undergo the Kasai PE received transplants and survived.

Deaths of Patients With BA

Four patients (8%, all with previous Kasai PE) died while waiting for LT. All of these deaths occurred before 2001; all of the patients had end-stage liver disease, including 1 who died from intracranial hemorrhage after minor head trauma, and 1 who died following a small bowel volvulus 5 months after undergoing the Kasai operation.

Prognostic Factors

Influence of Age at Kasai Operation

According to the age at Kasai operation, 4-year SNL was 75.0% ± 15.3% in patients who were operated on before 46 days (n = 9), 33.3% ± 10.3% for patients operated on between 46 and 75 days (n = 24), and 11.3% ± 10.6% if operated on after 75 days (n = 10) (P = 0.02) (Fig. 5).

FIG. 5
FIG. 5:
Influence of age at the Kasai PE on SNL.

No significant impact on SNL was demonstrated for the anatomical pattern of the extrahepatic biliary remnant (types 1–4), the polysplenia syndrome, and the caseload of the center performing the Kasai operation.

In a sample of 43 patients, 19 (44%) had ≥1 episodes of cholangitis, either suspected (fever, signs of sepsis, increasing cholestasis) or proven (positive blood cultures) after the Kasai operation: 8 of 17 patients with successful Kasai operation, 3 of 5 patients with intermediate result of the Kasai operation, and 8 of 21 patients with failed Kasai operation (P = NS).

DISCUSSION

In Western countries short-term clearance of jaundice can be achieved with the Kasai PE (10) in approximately 50% to 60% of children (11–13), one third of patients can survive with their native liver up to age 10 years (14–16), and one fourth up to 20 years (16,17). Thus, LT is needed when the Kasai operation fails, and the overall survival of these patients has reached up to 90% in industrialized countries (7). Despite these important gains, detailed national data on the outcome of patients with BA are available only in a few countries.

In this study, the epidemiological characteristics of BA in Switzerland were found to be similar to reported data in other European countries. The incidence of BA in Switzerland was found to be 1 in 17,800 live births (95% CI 1/13,900–1/24,800), whereas it varies between 1 in 14,000 and 1 in 20,000 in other European studies. According to these studies and our results, the incidence of BA in Europe is approximately 1 in 18,000 live births; 1 in 18,000 belongs to the confidence interval of every European study, and compilation of reported European data from six countries (France [2]; Norway [3]; the Netherlands [5]; United Kingdom [6]; Sweden [4]; and Switzerland) leads to a calculated incidence of 1 in 18,400 live births (95% CI 17,200–19,800). Table 2 summarizes the reported worldwide incidences of BA and outlines the high incidence of BA in the Pacific Ocean region (18). In Switzerland we did not find any regional variation in incidence, as well as no significant year or month variations, similar to studies with larger numbers of patients from France (1), the Netherlands (4), and the United Kingdom (19).

TABLE 2
TABLE 2:
Reported worldwide incidences of BA

Four-year SNL in patients who underwent the Kasai operation was 37.4% in our series, which is lower than the results obtained in European specialized centers (48% in Paris Bicêtre (20), 51% in the 3 supraregional British centers (Birmingham, Leeds, London) (11), 49% 5-year SNL in Madrid) (21). Caseloads of the centers were correlated with the results: in the United Kingdom, 2 studies (5,12) showed a wide variation in SNL according to the experience of the centers in the management of patients with BA. These findings led to the centralization of all British patients with BA in 3 pediatric liver units (for a British population of about 60 million people), which are able to manage the child from diagnosis to LT. This policy proved to be efficient, high level results now being obtained for all children nationwide (11). In France (same population of 60 million people), a similar discrepancy in results was observed according to the center's caseloads (22). This led to the creation of the French Observatory of Biliary Atresia, and the promotion of an increased collaboration between centers to standardize procedures and optimize results at the highest possible level. This policy has been less effective: SNL remained unchanged in the center performing >20 Kasai operations per year (48% 4-year SNL), improved from 31% to 45% in the centers performing 3 to 5 Kasai operations per year, and remained suboptimal in the centers performing ≤2 Kasai operations per year (20). In the present study, no significant difference in results between Swiss centers could be demonstrated. Nevertheless, the limited caseload in the whole country (population of 7.5 million people, 4–5 new BA cases per year) and the suboptimal results of the Kasai operation nationwide (4-year SNL of 37%) raise the question of a nationwide management of patients with BA, either along the British centralized or French collaborative types, to avoid unnecessary LTs in infancy and early childhood. Comparisons of SNL and overall patient survival show that among the patients alive at 4 years old, 60% have received transplants in Switzerland, 51% in France (20), and 43% in the United Kingdom (11).

Liver transplantation was needed in 31 of 48 children with BA, including 5 patients without previous Kasai operation. In our study 4 patients died while waiting for LT, all of them before 2001. Of note, before 2001 split liver grafts were not commonly used, and neither were LTs performed with parental living donors (Table 1). The advent of these techniques has notably reduced the shortage of organs for pediatric recipients and diminished the loss of patients on the waiting list; the rate of patients dying while waiting for a LT dropped from 22% before 2001 to 0% thereafter. This evolution has also been observed in the United Kingdom, where the mortality without LT diminished from 10.8% in the 1993–1995 national study (5) to 4.7% in the 1999–2002 study (11), and in France from 15.6% in the 1986–1996 study (22) to 7.0% in the 1997–2002 study (20). Survival after LT was excellent in the Swiss series: all of the patients who received transplants are alive.

Four-year OS of all patients with BA was 91.5% in Switzerland, fitting the international western standards. In Europe, 4-year OS was 87.3% in France (271 patients, 1997–2002) (20), and 89% in the United Kingdom (148 patients, 1999–2002) (11). In the Biliary Atresia Research Consortium series (104 patients from 9 US centers, 1997–2000), actual 2-year OS of patients with BA after Kasai operation was 91.3% (23). Five-year overall survival of all patients with BA was 77.3% in Canada (349 patients, 1985–2002) (24), 75.5% in Japan (1381 patients, 1989–1999) (25), and 41.9% in Taiwan (30 patients, 2002–2003), where LT is not as readily available as in Western countries (26).

The age at Kasai PE has been repeatedly shown to influence SNL in large series (22,27–30). There are also contradictory reports that fail to show the influence of the age at Kasai on success of the PE (5,31–33). As outlined by McKiernan et al, these results may be due to insufficient numbers of patients in the subgroups to be compared (5). Nevertheless, in our small series, the effect of age at Kasai operation appeared clearly. These results emphasize the need to diagnose BA early and promptly operate on these patients. In this Swiss series, the median age at Kasai operation was 68 days (range, 30–126), which is higher than in other recent series: 57 days (12–151) in France (20), 54 days (7–175) in the United Kingdom (11), and 61 days (11–153) in the US-BARC series (23). Therefore, efforts are needed to sensitize parents and health personnel to look at stool color, which is a simple method to detect neonatal cholestasis. At the national level, these efforts may lead to the use of a personal stool color card for each neonate, as in Japan (34), Taiwan (35), or Argentina (36). Moreover, every case of neonatal jaundice lasting >14 days should be explored to rule out cholestasis and biliary atresia (37). Even after the age of 3 months, however, appreciable chances of success of the Kasai operation do persist. In the French national series 1986–1996, 25% of the patients operated on after 3 months were alive with their native liver at 5 years (38). At the King's College Hospital, London, 5-year SNL was 45% in a series of patients with BA who were operated on after 100 days of age (39).

Our results as well as those of other studies show that postoperative clearance of jaundice is a strong indicator for the success of the Kasai PE and thus the need for LT (26,28,40). We failed to demonstrate any correlation between the degree of clearance of jaundice (complete, intermediate, none) and the incidence of episodes of cholangitis. However, cholangitis is known to impair the outcome after the Kasai PE (26,31,41–43), and every episode needs early and vigorous therapy. In contrast to other studies (22,44), the anatomical pattern of the extrahepatic biliary remnant (type 1 to 4), or the existence of a polysplenia syndrome were not found to have a significant impact on SNL after the Kasai operation. These negative results may be due to the small number of patients in the present study.

In conclusion, our study shows that overall survival of children with BA in Switzerland compares favorably with international standards, whereas results of the Kasai operation could be improved to reduce the need for LT in infancy and early childhood.

Acknowledgmnts:

The authors thank all of the pediatricians and surgeons of the 7 participating Swiss centers: University Hospital of Geneva, University Hospital of Basel (especially to R.I. Furlano), University Hospital of Berne (especially to B. Liniger, S. Schibli), University Hospital of Lausanne (especially to M. Roulet), Cantonal Hospital of Lucerne (especially to H. Winiker, J. Spalinger), Children's Hospital of St Gallen (especially to G. Marx), University Children's Hospital of Zurich (especially to C. Braegger and C.A. Gitzelmann). Thanks also to Frédéric Gauthier and Olivier Bernard (University Hospital of Paris Bicêtre, France), Elisabeth Aebischer (Swiss Federal Statistic Office, Neuchatel), and Matthieu Carton (INSERM U88, Saint Maurice, France).

REFERENCES

1. Chardot C, Carton M, Spire-Bendelac N, et al. Epidemiology of biliary atresia in France: a national study 1986–96. J Hepatol 1999; 31:1006–1013.
2. Henriksen NT, Drablos PA, Aagenaes O. Cholestatic jaundice in infancy. The importance of familial and genetic factors in aetiology and prognosis. Arch Dis Child 1981; 56:622–627.
3. Fischler B, Haglund B, Hjern A. A population-based study on the incidence and possible pre- and perinatal etiologic risk factors of biliary atresia. J Pediatr 2002; 141:217–222.
4. Houwen RH, Kerremans II, van Steensel-Moll HA, et al. Time-space distribution of extrahepatic biliary atresia in The Netherlands and West Germany. Z Kinderchir 1988; 43:68–71.
5. McKiernan PJ, Baker AJ, Kelly DA. The frequency and outcome of biliary atresia in the UK and Ireland. Lancet 2000; 355:25–29.
6. Kasai M. Treatment of biliary atresia with special reference to hepatic porto-enterostomy and its modifications. Prog Pediatr Surg 1974; 6:5–52.
7. Chardot C, Serinet MO. Prognosis of biliary atresia: what can be further improved? J Pediatr 2006; 148:432–435.
8. Chardot C. Biliary atresia. Orphanet J Rare Dis 2006; 1:28.
9. Schweizer P, Kirschner H, Schittenhelm C. Anatomy of the porta hepatis as a basis for extended hepatoporto-enterostomy for extrahepatic biliary atresia—a new surgical technique. Eur J Pediatr Surg 2001; 11:15–18.
10. Kasai M, Suzuki S. A new operation for “non-correctable” biliary atresia: hepatic porto-enterostomy. Shuiyutsu 1959; 13:733–739.
11. Davenport M, De Ville de Goyet J, Stringer MD, et al. Seamless management of biliary atresia in England and Wales (1999–2002). Lancet 2004; 363:1354–1357.
12. McClement JW, Howard ER, Mowat AP. Results of surgical treatment for extrahepatic biliary atresia in United Kingdom 1980–2. Survey conducted on behalf of the British Paediatric Association Gastroenterology Group and the British Association of Paediatric Surgeons. BMJ (Clin Res Ed) 1985; 290:345–347.
13. Gauthier F, Luciani JL, Chardot C, et al. Determinants of life span after Kasai operation at the era of liver transplantation. Tohoku J Exp Med 1997; 181:97–107.
14. Howard ER, Davenport M. The treatment of biliary atresia in Europe 1969–1995. Tohoku J Exp Med 1997; 181:75–83.
15. Karrer FM, Price MR, Bensard DD, et al. Long-term results with the Kasai operation for biliary atresia. Arch Surg 1996; 131:493–496.
16. Lykavieris P, Chardot C, Sokhn M, et al. Outcome in adulthood of biliary atresia: a study of 63 patients who survived for over 20 years with their native liver. Hepatology 2005; 41:366–371.
17. Howard ER, MacLean G, Nio M, et al. Survival patterns in biliary atresia and comparison of quality of life of long-term survivors in Japan and England. J Pediatr Surg 2001; 36:892–897.
18. Danks DM, Campbell PE, Jack I, et al. Studies of the aetiology of neonatal hepatitis and biliary atresia. Arch Dis Child 1977; 52:360–367.
19. Davenport M, Dhawan A. Epidemiologic study of infants with biliary atresia. Pediatrics 1998; 101:729–730.
20. Serinet MO, Broue P, Jacquemin E, et al. Management of patients with biliary atresia in France: results of a decentralized policy 1986–2002. Hepatology 2006; 44:75–84.
21. Lopez Santamaria M, Gamez M, Murcia J, et al. Kasai operation in the age of liver transplantation. Healing or merely palliative technique? Cir Pediatr 2000; 13:102–105.
22. Chardot C, Carton M, Spire-Bendelac N, et al. Prognosis of biliary atresia in the era of liver transplantation: French national study from 1986 to 1996. Hepatology 1999; 30:606–611.
23. Shneider BL, Brown MB, Haber B, et al. A multicenter study of the outcome of biliary atresia in the United States, 1997 to 2000. J Pediatr 2006; 148:467–474.
24. Schreiber R. Outcomes of biliary atresia in Canada. Screening and Outcomes of Biliary Atresia conference organized by the National Institutes of Health, Bethesda, MD, September 11–12, 2006.
25. Nio M, Ohi R, Miyano T, et al. Five- and 10-year survival rates after surgery for biliary atresia: a report from the Japanese Biliary Atresia Registry. J Pediatr Surg 2003; 38:997–1000.
26. Hung PY, Chen CC, Chen WJ, et al. Long-term prognosis of patients with biliary atresia: a 25-year summary. J Pediatr Gastroenterol Nutr 2006; 42:190–195.
27. Altman RP, Lilly JR, Greenfeld J, et al. A multivariable risk factor analysis of the portoenterostomy (Kasai) procedure for biliary atresia: twenty-five years of experience from two centers. Ann Surg 1997; 226:348–355.
28. Subramaniam R, Doig CM, Bowen J, et al. Initial response to portoenterostomy determines long-term outcome in patients with biliary atresia. J Pediatr Surg 2000; 35:593–597.
29. dos Santos JL, Cerski CT, da Silva VD, et al. [Factors related to the post-portoenterostomy prognosis of biliary atresia. J Pediatr (Rio J) 2002; 78:341–346.
30. Sangkhathat S, Patrapinyokul S, Tadtayathikom K, et al. Peri-operative factors predicting the outcome of hepatic porto-enterostomy in infants with biliary atresia. J Med Assoc Thai 2003; 86:224–231.
31. Wildhaber B, Coran AG, Drongowski RA, et al. The Kasai portoenterostomy for biliary atresia: a review of 27-year experience with 81 patients. J Pediatr Surg 2003; 38:1480–1485.
32. Tagge D, Tagge E, Drongowski R, et al. A long-term experience with biliary atresia. Reassessment of prognostic factors. Ann Surg 1991; 214:590–598.
33. Schoen BT, Lee H, Sullivan K, et al. The Kasai portoenterostomy: when is it too late? J Pediatr Surg 2001; 36:97–99.
34. Matsui A, Dodoriki M. Screening for biliary atresia. Lancet 1995; 345:1181.
35. Chen SM, Chang MH, Du JC, et al. Screening for biliary atresia by infant stool color card in Taiwan. Pediatrics 2006; 117:1147–1154.
36. Ramonet M. Stool color cards for screening for biliary atresia. Screening and Outcomes of Biliary Atresia conference organized by the National Institutes of Health, Bethesda, MD, September 11–12, 2006.
37. Hussein M, Howard ER, Mieli-Vergani G, et al. Jaundice at 14 days of age: exclude biliary atresia. Arch Dis Child 1991; 66:1177–1179.
38. Chardot C, Carton M, Spire-Bendelac N, et al. Is the Kasai operation still indicated in children older than 3 months diagnosed with biliary atresia? J Pediatr 2001; 138:224–228.
39. Davenport M, Puricelli V, Farrant P, et al. The outcome of the older (≥100 days) infant with biliary atresia. J Pediatr Surg 2004; 39:575–581.
40. Ohhama Y, Shinkai M, Fujita S, et al. Early prediction of long-term survival and the timing of liver transplantation after the Kasai operation. J Pediatr Surg 2000; 35:1031–1034.
41. Chittmittrapap S, Chandrakamol B, Poovorawan Y, et al. Factors influencing outcome after hepatic portoenterostomy for biliary atresia: a logistic regression analysis. J Med Assoc Thai 2005; 88:1077–1082.
42. Lunzmann K, Schweizer P. The influence of cholangitis on the prognosis of extrahepatic biliary atresia. Eur J Pediatr Surg 1999; 9:19–23.
43. Wu ET, Chen HL, Ni YH, et al. Bacterial cholangitis in patients with biliary atresia: impact on short-term outcome. Pediatr Surg Int 2001; 17:390–395.
44. Kobayashi H, Stringer MD. Biliary atresia. Semin Neonatol 2003; 8:383–391.
Keywords:

Biliary atresia; Epidemiology; Prognosis; Pediatric surgery; Liver transplantation

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