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O0048 EFFECT OF ZINC SUPPLEMENTATION ON LIVER NUTRITIONAL STATUS AND HEPATIC CONTENT OF ZINC, COPPER AND IRON

Speridião, P. G. L.1; Azevedo, R. A.1; Koh, I. H. J.1; Amancio, O. M. S.1; Morais, T. B.1; Patrício, F. R. S.1; Fagundes-Neto, U.1; Morais, M. B.1

Journal of Pediatric Gastroenterology and Nutrition: June 2004 - Volume 39 - Issue - p S26
ABSTRACTS: Oral Presentation Abstracts
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1Pediatric Gastroenterology, Federal University of São Paulo, São Paulo, Brazil

Submitted by: gastropediatria@msn.com

Introduction: To evaluate the effect of zinc supplementation on liver nutritional status and hepatic content of zinc, copper and iron.

Methods: Four groups of male Wistar rats newly weaned at 21 days of life were formed: 1) sham operated-30 (n=12),submitted to simulate surgery and fed with ration containing the recommended amount of zinc (30 mg/Kg); 2) operated-30 (n=11), submitted to bile duct ligature and transection, fed with ration containing the recommended amount of zinc (30 mg/Kg);3)sham operated-60 (n=12), submitted to simulated surgery and fed with ration containing twice the zinc recommendation (60 mg/Kg). The animals were fed their respective rations for 30 days, after wich liver weight, liver humity and hepatic zinc, copper and iron were evaluated.

Results: Liver weight was greater in the operated-60 group (11.58 ± 2.27 g) in relation to the sham operated-30 (7.75 ± 1.07 g), operated-30 (8.42 ± 1.11 g), and sham operated-60 (7.74 ± 1.07 g; p<0.001) groups. Hepatic zinc in the operated-60 group was 110.70 mug/g while in the other groups it ranged between 90.24 and 97.31 mug/g; however, the difference was not significant (p=0.444). The statistical analysis did not show difference in hepatic iron content, wich presented the following means: 119.45 ± 48.15 mug/g in the operated-30 group, 118.29 ± 49.13 mug/g in the operated-60 group, 123.83 ± 46.41 mug/g in the sham operated-30 group, and 148.44 ± 48.96 mug/g in the sham operated-60 group. There was no statistical difference between groups (p=0.395). Regarding the hepatic copper, it was observed the occurence of a greater concentration in the operated-30 and the operated-60 groups (143.94 mug/g and 147.82 mug/g, respectively) in relation to the sham operated-30 and sham operated-60 groups(17.95 mug/g and 15.95 mug/g, respectively), showing a statistically significant difference (p<0.001).

Conclusion: The animals submitted to the bile duct ligature presented compromised on liver nutritional status, as well as greater liver weight and hepatic copper concentration.

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Reference(S):

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