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O0047 WATER EXTRACT OF COLEUS BARBATUS COULD BE USED IN THE TREATMENT OF CHOLESTATIC AND IN THE MODULATION OF LIPID METABOLISM – EXPERIMENTAL STUDY IN YOUNG RATS

Battochio, A. R.1; Santos, A. G.1; Coelho, C. A. R.1

Journal of Pediatric Gastroenterology and Nutrition: June 2004 - Volume 39 - Issue - p S25
ABSTRACTS: Oral Presentation Abstracts
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1Departaments of Pediatrics, School of Medicine of Botucatu-Unesp, Botucatu, Brazil

Submitted by: apbattochio@ig.com.br

Introduction: Chronic obstructive cholestasis in young children is associated with an increase in serum levels of cholesterol and triacylglycerols. Extracts of Coleus barbatus are employed in brazilian folk medicine in the treatment of hepatic ailments. Our aim was to study, in an experimental model of biliary cirrhosis the interaction of an water extract of C. barbatus with hepatic function and nutrition in cholestasis

Methods: We studied forty Wistar rats weaned at P21 (21st postnatal day) and submitted, in groups of 10 to one of four treatments. G1-sham operation and daily administration, by gavage of water; G2-sham operation and daily administration, by gavage of water soluble extract of C. barbatus (60g of dry leaves extracted by 1L, volume given in both cases was 5mL/kg); G3-double ligature and ressection of the common bile duct and daily administration, by gavage of water; G4-double ligature and res-section of the common bile duct and daily administration by gavage of the same extract. At 48 we measured the sleeping time after injection of pentobarbital (50 mg/kg). At P49 when euthanasia was done the following variables were determined serum aminotransferases activities (U/L); total cholesterol and triacylglycerols concentrations (mg/dL); liver wet weight (g) and fat content of the liver (g/100g). Weight gain (g) from P29 to P49 was calculated. The datas was analyzed by the two way ANOVA test. Comparisons between groups were made using the S.N.K. test (0.05)

Results: Extract decreased pentobarbital sleeping time (median-M; G1=14; G2=5; G3=35; G4=25); liver wet weight (M; G1=24; G2=14; G3=35; G4=10); serum total cholesterol (M; G1=241; G2=96; G3=1260; G4=541) and triacylglycerols (M; G1=452; G2=163; G3=1312; G4=650) and fat liver content (M; G1=44; G2=16; G3=37; G4=9) in sham operated animals. Increase in pentobarbital sleeping time (M; G1=14; G2=5; G3=35; G4=25), aminotransferases (M; ALT - G1=12; G2=10; G3=34; G4=25; AST - G1=10; G2=11; G3=34; G4=26) liver wet weight, serum total cholesterol (M; G1=241; G2=96; G3=1260; G4=541) and triacylglycerols (M; G1=452; G2=163; G3=1312; G4=650), liver wet weight (M; G1=24; G2=14; G3=35; G4=10) and fat liver content (M; G1=44; G2=16; G3=37; G4=9) due to cholestasis was attenuated by the treatment with the extract. Weight gain was decreased in the animals receiving extract independently of their being cholestatic or not (Means, G1=169; G2=153; G3=105; G4=69)

Conclusion: Water extract of C. barbatus has a modulating effect on lipid metabolism in normal as well as in cholestatic liver disease. It also stimulated the CYP 450 in both situations demonstrated by hepatoprotective effect cholestatic

© 2004 Lippincott Williams & Wilkins, Inc.