Annual Meeting of the North American Society for Pediatric Gastroenterology and Nutrition; Orlando, October 22-24, 1998
INCREASED GASTRIC PRODUCTION OF PLATELET-ACTIVATING FACTOR, LEUKOTRIENE-B4, AND TUMOR NECROSIS FACTOR-α IN CHILDREN WITH HELICOBACTER PYLORI INFECTION
The concentrations of platelet-activating factor (PAF), leukotriene-B4 (LTB4), and tumor necrosis factor-α (TNF-α) in homogenate supernatants of gastric mucosal biopsy specimens and in gastric juice of Helicobacter pylori-positive (N=21) and -negative children (N=14) were investigated to determine whether these lipid mediators and cytokine are involved in the inflammatory reaction of H. pylori-associated gastritis. PAF and LTB4 concentrations were measured after high-performance liquid chromatography (HPLC) purification by specific radioimmunoassay, and TNF-α by using an enzyme-linked immunosorbent assay. The concentrations of PAF, LTB4, and TNF-α in gastric juice and biopsy specimens of children with H. pyloripositive gastritis were found to be statistically elevated and in positive correlation with each other. (Table)
This study suggested that increased local mucosal production of potent proinflammatory agents such as PAF, LTB4, and TNF-α may be implicated in the pathogenesis of H. pylori-associated gastritis in childhood.
Transport/Cell Biology/Nutrition© 1998 Lippincott Williams & Wilkins, Inc.