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Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota

An Observational Pilot Study

Cabrera-Rubio, Raul*; Kunz, Clemens; Rudloff, Silvia†,‡; García-Mantrana, Izaskun*; Crehuá-Gaudiza, Elena; Martínez-Costa, Cecilia; Collado, M. Carmen*,§

Journal of Pediatric Gastroenterology and Nutrition: February 2019 - Volume 68 - Issue 2 - p 256–263
doi: 10.1097/MPG.0000000000002216
Rapid Communication: Nutrition

Background and Objectives: Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota. HMO profile is determined by secretor status; however, their influence on milk microbiota is still uncovered. This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.

Methods: Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing. Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.

Results: The most abundant bacteria were Staphylococcus and Streptococcus, followed by Enterobacteriaceae-related bacteria. The predominant HMO in secretor milk samples were 2’FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II. Differences in microbiota composition and quantity were found depending on secretor/non-secretor status. Lactobacillus spp, Enterococcus spp, and Streptococcus spp were lower in non-secretor than in secretor samples. Bifidobacterium genus and species were less prevalent in non-secretor samples. Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.

Conclusions: Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks. Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive. Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.

*Department of Biotechnology, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain

Institute of Nutritional Science

Department of Pediatrics, Justus-Liebig-University, Giessen, Germany

§Department of Pediatrics, Hospital Clinico Universitario, Nutrition Research Group of INCLIVA, University of Valencia, Spain.

Address correspondence and reprint requests to M. Carmen Collado, PhD, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Av. Agustin Escardino 7, 46980 Paterna, Valencia, Spain (e-mail: mcolam@iata.csic.es).

Received 2 July, 2018

Accepted 13 November, 2018

Supplemental digital content is available for this article. Direct URL citations appear in the printed text, and links to the digital files are provided in the HTML text of this article on the journal's Web site (www.jpgn.org).

I.G.M. and M.C.C. would like to acknowledge the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (ERC Starting grant, project no. 639226).The authors report no conflicts of interest.

Raul Cabrera-Rubio, Actual address: Food Biosciences Department, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland and APC Microbiome Institute, University College Cork, Cork, Ireland.

© 2019 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology,