Original Articles: Clinical Studies: PDF OnlyEfficient Storage System for Breath HydrogenMurray, Robert D.*; Kerzner, Benny*; MacLean, William C. Jr.; McClung, H. Juhling*; Gilbert, Merry Author Information *Department of Pediatrics, College of Medicine, The Ohio State University, Journal of Pediatric Gastroenterology and Nutrition: October 1985 - Volume 4 - Issue 5 - p 711-713 Free Abstract Summary: Recommended materials for breath hydrogen collection (plastic syringes with twist lock closure) are only adequate for relatively brief periods because of gradual hydrogen loss and considerable variability between duplicate samples. To document the most favorable storage conditions for breath hydrogen, we compared hydrogen retention in plastic syringes using a conventional twist-in-lock closure versus a simple, inexpensive syringe closure, a Critocap. Hydrogen retention was studied at 25, 5, and – 20°C in two different syringe brands over 72 h of storage. An analysis of variance confirms the superiority of Critocaps over twist-in-lock closures (p < 0.001). Reliability was maximal when samples were placed in environments <5°C. When storage time was extended to 7 days, mean hydrogen retention was 86 ± 6% (± SD). © Lippincott-Raven Publishers.