Review ArticlesThe Role of Radiolabeled Monoclonal Antibodies in Cancer Imaging and ADC TreatmentMartiniova, Lucia PhD∗; Zielinski, Rafal J. PhD∗; Lin, Mai PhD†; DePalatis, Louis PhD‡; Ravizzini, Gregory C. MD§ Author Information From the ∗Department of Experimental Therapeutics †Cyclotron Radiochemistry Facility, The University of Texas MD Anderson Cancer Center, Houston, TX ‡BioDevelopment Solutions, LLC, Johnstown, CO §Department of Nuclear Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX. Conflicts of Interest and Source of Funding: G.C.R. is a recipient of research support from FUJIFILM Healthcare, Amgen, Novartis, Bayer, Blue Earth Diagnostics, ABX, and Curium Pharma. For the remaining authors, none were declared. Reprints: Gregory C. Ravizzini, MD, Department of Nuclear Medicine, The University of Texas MD Anderson Cancer Center, 1400 Pressler St, Houston, TX 77030. E-mail: [email protected]. The Cancer Journal 28(6):p 446-453, 11/12 2022. | DOI: 10.1097/PPO.0000000000000625 Buy Metrics Abstract Antibody-drug conjugates (ADCs) are designed to deliver cytotoxic payloads to distinctive target-expressing cancer cells. Following internalization, the ADCs are routed to different compartments in the cells, where cleavage of the linker causes release of the cytotoxic cargo. With such a delivery system, more effective payloads can reach cancer cells, allowing for more efficient treatment and dosing schedule. The monoclonal antibody (mAb) component of ADC plays a crucial role in the effective targeting of cancer cell–specific antigens while minimizing binding to normal cells. Often, the same mAbs used in ADCs can be labeled instead with radionuclides suitable for positron emission tomography or gamma-camera scintigraphy. To achieve high sensitivity and specificity for imaging, radiolabeled mAbs must have high affinity for the antigen, favorable pharmacokinetic properties, and a low toxicity profile. The use of radiolabeled mAbs permits the noninvasive interrogation of specific target expression on tumor cells and assessment of tumor heterogeneity in vivo by a simple diagnostic imaging scan that may include the whole body in the field of view. With this approach, radiolabeled mAbs can serve as important imaging biomarkers to predict the optimal delivery of ADCs to tumors and be used to monitor therapy with follow-up scans. Moreover, the same mAb can then be radiolabeled with an analogous radionuclide for the delivery of β-emitters, α-particles, or Auger electrons as part of a radioimmunotherapy approach. The purpose of this review is to introduce key concepts regarding radiolabeled mAbs targeting various tumor antigens (CD20, CDH3, type I insulinlike growth factor receptor, prostate-specific membrane antigen, and human epidermal growth factor receptor 2) that are being used in the clinical setting or undergoing development. Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.