Article: PDF OnlyStafford H.; Oni, O. O. A.; Hay, J.; Gregg, P. J.Journal of Orthopaedic Trauma: June 1992 - p 190-194 Buy Abstract SummaryThe extraosseous tissue contribution to diaphyseal fracture callus has been investigated using a rabbit tibial fracture model and osteocalcin immunocytochemistry. The extraosseous tissues were isolated for study by reaming and nailing an osteotomy and excising 2 cm of periosteum on either side of the osteotomy. Specimens obtained from the healing fractures at 1 and 2 weeks after operation, respectively, were decalcified and stained for osteocalcin, a bone-specific protein, using an indirect immunoperoxidase method. The positively stained osteogenic cells appeared to be derived exclusively from the remnant of the periosteum. © Lippincott-Raven Publishers.