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Sevoflurane Postconditioning Reduces Apoptosis by Activating the JAK-STAT Pathway After Transient Global Cerebral Ischemia in Rats

Kim, Hyun-Chang MD; Kim, Eugene MD; Bae, Jung Il MD; Lee, Kook Hyun MD, PhD; Jeon, Young-Tae MD, PhD; Hwang, Jung-Won MD, PhD; Lim, Young-Jin MD, PhD; Min, Seong-Won MD, PhD; Park, Hee-Pyoung MD, PhD

Journal of Neurosurgical Anesthesiology: January 2017 - Volume 29 - Issue 1 - p 37–45
doi: 10.1097/ANA.0000000000000331
Laboratory Investigations
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Background: The antiapoptotic effects of sevoflurane postconditioning are responsible for neuroprotection against cerebral ischemia-reperfusion injury. Phosphorylation of the Janus family tyrosine kinases (JAK) 2-signal transducers and activators of transcription (STAT) 3 pathway is linked to antiapoptosis. Here, we determined whether the antiapoptotic effects of sevoflurane postconditioning are associated with activation of the JAK2-STAT3 pathway after global transient cerebral ischemia in rats.

Materials and Methods: Forty-five rats were randomly assigned to 5 groups: sham (n=5), control (10 min of ischemia, n=10), sevoflurane postconditioning (2 periods of sevoflurane inhalation after ischemia for 10 min, n=10), AG490 (a JAK2 selective inhibitor, intraperitoneal administration of 40 mg/kg before ischemia, n=10), and sevoflurane postconditioning plus AG490 group (n=10). The number of apoptotic cells as well as the expression of JAK2, phosphorylated JAK2 (P-JAK2), STAT3, phosphorylated STAT3 (P-STAT3), Bcl-2 (antiapoptotic protein), and Bax (proapoptotic protein) were evaluated 3 days after ischemia.

Results: The apoptotic cell count was significantly lower in the sevoflurane postconditioning group than in the control, AG490, and sevoflurane postconditioning plus AG490 groups. JAK2 and STAT3 levels were comparable among all 5 groups. P-JAK2, P-STAT3, and Bcl-2 levels were higher and Bax levels were lower in the sevoflurane postconditioning group relative to the control, AG490, and sevoflurane postconditioning plus AG490 groups.

Conclusions: Sevoflurane postconditioning reduced apoptosis by increasing P-JAK and P-STAT expression after transient global ischemia in rats, and AG490 reversed the beneficial antiapoptotic effects of sevoflurane postconditioning, suggesting that the JAK-STAT pathway may be involved in the antiapoptotic mechanism of sevoflurane postconditioning.

*Department of Anesthesiology and Pain Medicine, Keimyung University Dongsan Hospital, Keimyung University College of Medicine

Department of Anesthesiology and Pain Medicine, Hospital of Catholic University of Daegu, Daegu

Department of Anesthesiology and Pain Medicine, Seoul National University Hospital

Department of Anesthesiology and Pain Medicine, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul

§Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea

H.-P.P. and S.-W.M. are co-corresponding authors.

Supported by research grants funded by Seoul National University Hospital (number: 0420130360) and Keimyung University (number: 2015-0170).

The authors have no conflicts of interest to disclose.

Address correspondence to: Hee-Pyoung Park, MD, PhD, Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehakro, Jongno-gu, Seoul 03080, Korea (e-mail: hppark@snu.ac.kr) and Seong-Won Min, MD, PhD, Department of Anesthesiology and Pain medicine, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, 5-20 Boramae-ro, Dongjak-gu, Seoul 07061, Korea (e-mail: swmin@snu.ac.kr).

Received March 21, 2016

Accepted May 20, 2016

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