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Original Contribution

Validity of Forced Eyelid Closure Test: A Novel Clinical Screening Test for Ocular Myasthenia Gravis

Apinyawasisuk, Supanut MD; Zhou, Xinkai MS; Tian, Jack J.; Garcia, Giancarlo A. AB; Karanjia, Rustum MD, PhD; Sadun, Alfredo A. MD, PhD

Author Information
Journal of Neuro-Ophthalmology: September 2017 - Volume 37 - Issue 3 - p 253-257
doi: 10.1097/WNO.0000000000000514

Abstract

Patients with ocular myasthenia gravis (OMG) typically present with clinically fatigable ptosis and/or binocular diplopia (1). In general, noninvasive clinical tests such as lid fatigability, ice test, rest test, and Cogan lid twitch (CLT) test are helpful screening tools to guide further diagnostic testing for OMG. Commonly used and well-studied diagnostic studies include the edrophonium test, serum acetylcholine receptor antibody (AchR Ab), single-fiber electromyography (SFEMG), and repetitive nerve stimulation (RNS). However, all diagnostic tests have varying levels of sensitivity and specificity (2).

In our clinic, forced eyelid closure test (FECT) routinely is performed in patients suspected of having OMG. This test was first described in the early 1980s by Don C. Bienfang, MD, a neuro-ophthalmologist at Harvard Medical School, and was formerly named “Bienfang test.” The primary objective of our study was to evaluate whether FECT achieved a minimally acceptable sensitivity and specificity, both set to 0.8. A secondary objective was to compare the sensitivity and specificity of FECT to CLT.

METHODS

We retrospectively reviewed the electronic medical records of patients who presented at our neuro-ophthalmology clinic at Doheny Eye Institute, University of California, Los Angeles from February 2015 to April 2016, with ptosis or binocular diplopia as a chief complaint. We included all patients who were tested with FECT and had test results available. To perform the test, the patient was asked to tightly squeeze his or her eyelids shut for 5–10 seconds then open quickly and fixate at a target positioned in primary gaze. The observer sat 2–3 feet in front of the patient at the same eye level and immobilized the eyebrows with digital pressure to minimize the contribution of the frontalis muscle. A positive test was defined by an excessive upward overshoot of the eyelid on opening followed by a downward drooping to arrive at the final position (Fig. 1) (see Supplemental Digital Content, Video, http://links.lww.com/WNO/A238). The test was performed by an experienced neuro-ophthalmologist (AAS). In addition, we collected the CLT result. To perform CLT, the patient was asked to look downward for at least 10 seconds and rapidly return to primary gaze. Excessive upward eyelid movement followed by downward drooping was considered a positive CLT.

FIG. 1.
FIG. 1.:
Forced eyelid closure test. A series of schematic drawings illustrate activities of the right levator palpebrae superioris (LPS), tarsal plate (TP) and orbicularis oculi (OO) (A). The arrows illustrate direction of force generated by each muscle or direction of movement of the muscle. During the squeezing step (B), OO tensely contracts while LPS fully relaxes. On immediate eyelid opening (C), the balance of the LPS and its antagonist (OO) is altered in favor of LPS (because of fatiguing of OO) allowing momentary upward overshoot of the eyelid. This recovery is short lived as LPS fatigues and the lid comes down (D).

The final diagnosis of each patient was recorded. The diagnosis of OMG was made when at least one of the standard diagnostic tests for myasthenia gravis was positive. These included edrophonium test, AchR Ab, muscle-specific receptor tyrosine kinase, SFEMG, RNS. Because these tests have varying levels of sensitivity for OMG, patients with negative diagnostic test results received an OMG diagnosis if they demonstrated clinical characteristics highly specific for OMG such as dramatic responsiveness to oral pyridostigmine or were later documented to progress to generalized myasthenia gravis. The results of FECT and CLT were not used for establishing the final diagnosis. Exclusion criteria were patient with equivocal test results, and inconclusive final diagnosis. The study protocol was approved by the University of California, Los Angeles Institutional Review Board. Informed consent was exempted for this retrospective review.

Statistical Analysis

FECT and CLT results were cross-classified by OMG diagnosis in Table 1; true-positive fraction (TPF) and false-positive fraction (FPF) were calculated. We took and , and we used TPF and FPF for the analysis.

TABLE 1.
TABLE 1.:
Cross-classification of FECT and CLT results by OMG diagnosis

For the first objective, whether FECT achieves minimally acceptable sensitivity and specificity (both set to 0.8), we formulated a joint hypothesis that simultaneously test both parameters (3). The null hypothesis is and we performed hypothesis testing by constructing a joint 95% confidence region for the pair (TPF, FPF). We reject the null hypothesis at 0.05 level if the confidence region lies entirely in the rejection region .

For the second objective, comparing FECT with CLT, we performed hypothesis testing on relative TPF (rTPF) and relative FPF (rFPF), calculated as and . The model for TPF is , where “Test” is a binary covariate that equals 0 for CLT and 1 for FECT, such that . The model for FPF is similar. Model coefficients were estimated by the generalized estimating equations (4) to allow for correlations between test results from the same subject. Table 2 summarizes estimated and its 95% confidence interval; Table 2 summarizes results. The advantage of the above approach is that it allows us to use data from all 48 patients to obtain more efficient estimates of TPF and FPF for FECT, although the CLT test results are not available for 13 of them. As a sensitivity analysis, we also peformed McNemar test for TPF and FPF on the 35 subjects who have test results for both FECT and CLT.

TABLE 2.
TABLE 2.:
Regression model fit for FECT and CLT results using the model for TPF and the model for FPF

RESULTS

Electronic medical records of 57 patients initially were reviewed. One patient was excluded from the study because of an equivocal result for FECT and 8 patients were excluded because of inconclusive diagnosis. A total of 48 patients were included. Eighteen of these 48 patients (37.5%) demonstrated a positive FECT. Of the 18 patients, 15 patients (83.3%) had a final diagnosis of OMG. Of the 30 patients with negative FECT, 1 had OMG (3.3%). Of the 48 patients, 35 patients (72.9%) had an available CLT result. CLT was positive in 11 of 35 patients (31.4%), 9 of whom had a final OMG diagnosis (81.8%). Of the 24 patients with negative CLT, 2 patients had OMG (8.3%). The estimate TPF of FECT was 0.94 (94% sensitivity), and FPF was 0.09 (91% specificity) with joint 95% confidence region: TPF × FPF = (0.70, 1) × (0, 0.25) (see Supplemental Digital Content, Figure E1, http://links.lww.com/WNO/A239). The estimated TPF of CLT was 0.82 (82% sensitivity) and FPF was 0.08 (92% specificity). To compare FECT to CLT, the rTPF was 1.15; the rFPF was 1.31. However, the results were not statistically significant at the 0.05 level for either TPF or FPF (Table 2). We reached the same conclusion from the sensitivity analysis that used the McNemar test. Final diagnosis and clinical presentations of patients in positive and negative FECT groups are shown in Table 3.

TABLE 3.
TABLE 3.:
Clinical findings and final diagnosis of patients with positive and negative FECT

DISCUSSION

This is the first study evaluating the validity of FECT as a clinical diagnostic test for OMG. FECT provides high sensitivity and specificity for OMG diagnosis. However, comparison of FECT with CLT showed no statistically significant difference. Previous studies assessing CLT (5–7) showed trends toward low sensitivity (50%–75%) and high specificity (91.7%–100%) when compared with standard diagnostic tests, and the CLT results from this study are in agreement. However, comparison of our sensitivity and specificity of CLT to the results of previous studies is limited because of different study populations.

The explanation for eyelid phenomenon in both FECT and CLT remains unproven. In 1965, Cogan (8) first introduced CLT as a characteristic eyelid sign of myasthenia gravis and proposed fatigability followed by increased gain and then a rapid recovery of extraocular muscles including LPS as an explanation for this phenomenon. When the patient's eyes return to primary position after the period of LPS relaxation on downgaze, the eyelid shoots upward excessively for a brief moment exposing the upper limbus because of rapid recovery of acetylcholine levels in the context of compensatory gain before returning to resting position, and appears as a twitch. To further explain the phenomenon, we speculate that during the relaxation period, acetylcholine level builds up in the presynaptic junction of the nerve terminal before being released and then activates the remaining acetylcholine receptors free from blockage by autoantibodies at the postsynaptic junction of the LPS. The over-activated LPS contracts and excessively lifts the eyelid. The eyelid phenomenon in FECT can be demonstrated even in patients without ptosis (Fig. 1).

Although comparison of FECT with CLT showed no statistical significance in regard to sensitivity and specificity, we believe that FECT might have a greater sensitivity than CLT. This is supported by 2 possibilities. First, the contraction of orbicularis oculi (OO) (main eyelids protractor) during forced eyelid closure performed in FECT allows full relaxation of LPS (main eyelid retractor, OO's antagonist), whereas sustained downgaze performed in CLT only allows partial LPS relaxation. This allows for a greater upward drift when LPS subsequently contracts. Second, there may be OMG-related fatiguing of OO. In both situations, the balance of LPS and its antagonist (OO) is altered in favor of LPS allowing momentary recovery. This recovery is short lived as LPS fatigues and the compensatory extra gain passes and the lid comes down again.

The false-negative rate of FECT was low in our study. Among 16 patients in the OMG group, only 1 had a false-negative FECT. The final diagnosis was confirmed by 2 diagnostic tests (AchR Ab and RNS). At the time this false-negative FECT was performed, the patient had been treated elsewhere with pyridostigmine and oral corticosteroids.

Our study population contained 3 false-positive FECTs. Among those 3 patients, 2 were diagnosed with Lambert–Eaton myasthenic syndrome (LEMS). Given that LEMS is an autoimmune disorder that affects neuromuscular transmission and associated fatigable extraocular muscle weakness (9), it comes as no surprise that a positive FECT can be expected in this condition. Other clinical signs of OMG, including enhanced ptosis and CLT also have been reported in LEMS (9,10). Because the principle underlying both FECT and CLT is based on abnormality of acetylcholine level and AchR function, a positive FECT would possibly be expected in any disorder of neuromuscular transmission, including other myasthenic syndromes.

There were a number of limitations to our study. First, the sample size was too small to obtain statistically significant TPF and FPF. Second, CLT was not performed on all the patients. Third, the examiner was not completely masked to the patient's symptoms and/or diagnosis before performing the test. Fourth, the positivity and negativity of FECT was determined by an experienced neuro-ophthalmologist. Inexperienced observers might not be able to make a decision whether the test is positive or negative. Because this is a pilot study, a prospective masked study should be performed with a large sample size and with more observers with varying levels of experience. Moreover, interobserver reliability needs to be addressed in any future study. There is some ascertainment bias from the exclusion of uncertain results in our study. Some of the excluded patients had positive FECT but the diagnosis of OMG could not be established for a number of reasons.

In conclusion, FECT, formerly called Bienfang test is a simple, quick, noninvasive test and should be used as a valuable screening tool for OMG. Compared with CLT, it is noninferior with regard to sensitivity and specificity.

STATEMENT OF AUTHORSHIP

Category 1: a. Conception and design: S. Apinyawasisuk, X. Zhou, R. Karanjia, and A. A. Sadun; b. Acquisition of data: S. Apinyawasisuk, J. J. Tian, and G. A. Garcia; c. Analysis and interpretation of data: X. Zhou and A. A. Sadun. Category 2: a. Drafting the manuscript: S. Apinyawasisuk and X. Zhou; b. Revising it for intellectual content: J. J. Tian, G. A. Garcia, R. Karanjia, and A. A. Sadun. Category 3: a. Final approval of the completed manuscript: S. Apinyawasisuk, X. Zhou, J. J. Tian, G. A. Garcia, R. Karanjia, and A. A. Sadun.

ACKNOWLEDGMENTS

The authors acknowledge Professor Don C. Bienfang at Harvard Medical School for inventing the test and Shellee Rockwell (Doheny Eye Institute) for help in data collection.

REFERENCES

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Images in Neuro-Ophthalmology

Six months previously, a 65-year-old woman underwent a right penetrating keratoplasty.
Six months previously, a 65-year-old woman underwent a right penetrating keratoplasty.:
She developed pain and elevated intraocular pressure postoperatively and was noted to have a large, nonreactive right pupil (A). Iris fluorescein angiography of the right eye demonstrates impaired perfusion, particularly of the pupillary sphincter muscle (B). The left pupil is normal in appearance (C), as is angiography of the left iris (D). Ischemia of the iris is thought to be the cause of Urrets-Zavalia syndrome. (Courtesy of Sudip Thakar, Alex Young, Rod Foroozan. Houston, TX).

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