This cross-sectional study was intended to assess the association between immunohistochemical analysis of p16INK4A
and fragile histidine triad (FHIT
) and the presence of precancerous cervical lesions.
Materials and Methods.
Women seen at Pérola Byington Hospital, São Paulo, Brazil, with histologically confirmed cervicitis (n
= 31), cervical intraepithelial neoplasia (CIN) 1 (n
= 30), CIN 2,3 (n
= 30), and cervical cancer
= 7) had also cervical material collected for liquid-based cytology
, human papillomavirus Hybrid Capture 2 (HC2) test, and p16
reactions were scored as the following: <1%, 1% to 5%, >5% to 25%, and >25%. Receiver operating curve analysis was used to select p16
score cutoffs for further categorical analyses. All but one of the 37 CIN 2,3/cancer cases had a p16
score of greater than 1% to 5%. Among the 61 cervicitis/CIN 1 cases, 46 (75%) had a p16
score lower than 1% to 5%. In contrast, no association of FHIT
expression and severity of cervical lesions could be demonstrated in this data set. Receiver operating curve analyses suggested the score of 1% to 5% for p16
as the cutoff that best discriminates CIN 2,3/cancer from cervicitis/CIN 1. No cutoff for FHIT
scores could be suggested with data set.
, but not FHIT
expression, has the potential to be used as complementary diagnostic tool to investigate human papillomavirus-induced cervical lesions, if these results are confirmed in larger studies.