Smoking is a leading cause of death . Growing evidence suggests that the harm from smoking tobacco is not confined to ‘active’ smokers. Passive smoking, also known as secondhand smoke exposure (SHSE), is the inhalation of smoke by persons other than the intended ‘active’ smoker, and it is estimated to cause 331 000 deaths worldwide [2,3].
Cardiovascular disease is the main cause of premature death associated with SHSE . Two-thirds of all deaths attributable to SHSE were caused by ischemic heart disease . Studies have shown that SHSE increases the risk of coronary heart disease (CHD) by 25–30% [6,7]. There are several explanations for the link between SHSE and cardiovascular effects, including impaired autonomic regulation, impaired diastolic function, and increased inflammation . However, there is a paucity of data regarding the effects of SHSE on cardiovascular risk factors such as hypertension, diabetes, dyslipidemia, and fasting glucose .
SHSE is usually assessed using self-report questionnaires. However, this subjective method is prone to various sources of bias and cotinine levels can be used as objective markers [10,11]. Cotinine, the main metabolite of nicotine, can be measured in serum, urine, and saliva . It has a long half-life and is useful in quantifying not only active but also passive exposure to tobacco smoke . In this study, we evaluated the relationship of SHSE with cardiovascular risk factors, such as blood pressure (BP), lipid, or fasting glucose levels, in healthy Korean adults who had never smoked. The status of SHSE was assessed subjectively by self-report questionnaires as well as objectively by urine cotinine concentrations, quantifying the amount of passive exposure to tobacco smoke.
We performed a cross-sectional study using data from the Korea National Health and Nutrition Examination Survey (KNHANES) from 2008 to 2011. In brief, KNHANES is a nationwide representative survey in Korea using a complex, stratified, multistage, clustered-sampling design, which is used to examine the general health and nutritional status of the entire Korean population . We extracted data on 7376 men and women who had never smoked and whose urine cotinine levels were available from the 37 753 individuals in the database. Exclusion criteria included those aged 18 years or younger (n = 9376), currently pregnant (n = 54), who had a history of CHD or stroke (n = 212), or who had urine cotinine levels higher than 100 ng/ml (n = 306) (Fig. 1) [15–17]. Baseline characteristics of the participants included in this study were compared with those who were excluded are shown in Supplement Table 1, http://links.lww.com/HJH/A789.
SHSE status was assessed subjectively using a self-report questionnaire and objectively using results from a urinary cotinine assay. Information on age, sex, alcohol intake, income, education level, and cigarette smoking habits was obtained using standardized questionnaires during a home interview performed by trained medical personnel. BMI was categorized as normal (≥18.5 and <25 kg/m2), overweight (≥25 kg/m2), or obese (≥30 kg/m2). Educational attainment was categorized as not being a high school graduate (lower) or being a high school graduate or above (higher). Income status was categorized into quartiles according to medical insurance premiums that are closely correlated with an individual's yearly income status. Alcohol consumption was categorized as never, mild-to-moderate (two to four drinks per month), or heavy drinking (two to three drinks per week). Regular physical activity was defined as performing vigorous physical activity more than three times per week.
Health examination procedures were performed based on standardized protocols by trained medical personnel. All equipment was calibrated periodically. Height and body weight were measured using digital scales. BP was measured three times on the right arm using an appropriately sized arm cuff and mercury sphygmomanometer (Baumanometer; WA Baum Co., New York, New York, USA) after the study participant was at rest in a seated position for at least 5 min. The final BP value was obtained by averaging the second and third measurements .
Blood and urine samples were collected from participants to obtain laboratory tests . Blood samples were collected from the antecubital vein after 10–12 h of fasting. All biochemical analyses were performed within 2 h of blood sampling, and laboratory performance was monitored regularly by a data quality control program. Total cholesterol (TC), HDL cholesterol, LDL cholesterol, triglyceride, and glucose were measured with enzymatic methods using a Hitachi 7600 automatic analyzer (Hitachi Instruments Inc, Tokyo, Japan) or COBAS 8000 C702 (Roche, Mannheim, Germany) . Urine cotinine level was measured with gas chromatography–mass spectrometry using the Perkin Elmer Clarus 600T (PerkinElmer, Turku, Finland) . Urine cotinine levels were treated as both continuous variables and categorical variables. As a categorical variable, urine cotinine levels were coded into quartiles (Q1: 0.009–0.71 ng/ml, Q2: 0.72–3.90 ng/ml, Q3: 3.91–12.00 ng/ml, and Q4: 12.01–99.52 ng/ml); the lowest quartile was considered the reference. When treated as a continuous variable, urine cotinine levels were log-transformed because of their skewed distribution.
The outcome variables were SBP, DBP, lipid profiles including TC, HDL cholesterol, LDL cholesterol, triglyceride levels, and fasting glucose. Hypertension was defined as SBP at least 140 mmHg, DBP at least 90 mmHg, or taking antihypertensive drugs. Dyslipidemia was defined as HDL cholesterol less than 40 mg/dl, LDL cholesterol at least 160 mg/dl, triglyceride at least 200 mg/dl, or taking cholesterol-lowering drugs. Diabetes was defined as fasting glucose level at least 126 mg/dl, use of antidiabetic medication or insulin, or diagnosis of diabetes by physicians.
Data were presented as mean ± standard error (SE) or % (SE). Sampling weights based on the sample design of each KNHANES were used for all statistical analyses . Baseline characteristics were compared using two sample t tests for continuous variables and chi-square test for categorical variables. Considering the complex survey design of KNHANES, linear and logistic models were used with survey-weighted generalized linear models. The linear regression models were constructed to calculate the association between dependent and independent variables, and the logistic regression models were for the association of SHSE and dichotomous dependent variables. We performed multivariable-adjusted analyses. The first model was adjusted for age, sex, and BMI, whereas the second model was adjusted for education (higher versus lower), low-income status, alcohol consumption, regular physical activity, sodium intake, total caloric intake, fat proportion among total calories, and the variables included in the first model. When analyzing BP, individuals who were taking antihypertensive medications were not included in the model to eliminate the effects of the drugs. Similarly, those taking cholesterol-lowering agents and antidiabetic medications were excluded from the analysis of lipid levels and fasting glucose, respectively. Sensitivity analyses were also performed, including all study participants regardless of medication status, which has been adjusted. The association between urine cotinine and cardiovascular risk factors were stratified by SHSE at home and at work, which results were presented as another sensitivity analysis. All analyses were conducted using SAS (version 9.2; SAS Institute, Cary, North Carolina, USA) software and ‘survey’ package of the R program (R Foundation, Vienna, Austria). Two-sided P values less than 0.05 were considered statistically significant.
Baseline characteristics of the study population are shown in Table 1. None of the individuals included in the analysis were active or former smokers. The mean age of our population was 45.4 ± 0.4 years, 75.2% were women and 6.6% of population had diabetes mellitus. The mean calorie intake, sodium consumption, and fat proportion among total calories of this study population were 1790.0 ± 12.7 kcal, 4489.5 ± 43.7 mg, and 17.4 ± 0.1%, respectively. The mean SBP and DBP were 115.3 ± 0.3 and 75.3 ± 0.2 mmHg, respectively. The mean TC, HDL, LDL, and triglyceride were 186.0 ± 0.6, 50.0 ± 0.2, 113.8 ± 0.5, and 94.0 (92.4–96.0) mg/dl, respectively. The differences in baseline characteristics between individuals included in this study and those who were excluded are described in Supplement Table 1, http://links.lww.com/HJH/A789.
The status of SHSE was assessed subjectively and objectively. Table 2 describes the results of SHSE when assessed subjectively by self-report questionnaires. For most dependent variables, no significant associations were observed except for DBP, which had marginally positive associations (P = 0.060). When dependent variables were coded binomially, that is, hypertension, low HDL, high LDL, high triglyceride, dyslipidemia, and diabetes mellitus, there were still no significant associations with subjectively accessed SHSE (Supplemental Table 2, http://links.lww.com/HJH/A789).
Table 3 shows the results of the unadjusted and adjusted models in which participants were divided into quartiles according to their urine cotinine levels. Unadjusted analysis showed that higher cotinine levels were significantly associated with lower SBP, TC, LDL cholesterol, and triglyceride. After multivariable adjustment, all associations lost statistical significance, whereas increasing cotinine showed a positive relationship with higher fasting glucose. When the analysis was performed stratified according to sex, the results were similar among women (Supplemental Table 3, http://links.lww.com/HJH/A789). Among male participants, no relationship was statistically significant.
Analyses were also performed using logarithm-transformed urine cotinine levels treated as a continuous variable (Table 4). None of the dependent variables were significantly associated with urine cotinine levels in the unadjusted model. After multivariable adjustment, serum triglyceride has a negative relationship with urine cotinine. However, after further analysis with adjusted model 2, the relationship lost statistical significance. When stratified according to sex, LDL cholesterol was positively correlated with urine cotinine among men. SBP, cholesterol, LDL cholesterol, and triglyceride showed a significant relationship with logarithm-transformed cotinine among women, but lost statistical significance after multivariable adjustment (Supplemental Table 4, http://links.lww.com/HJH/A789).
Sensitivity analysis was performed without excluding participants who were taking medications that can affect the values of dependent variables and with adjustment for medication status. They showed mostly similar results with the main analysis. When urine cotinine was divided into quartiles, SBP decreased with higher cotinine, whereas fasting glucose had no significant relationship (Supplemental Table 5, http://links.lww.com/HJH/A789). Log-transformed cotinine was not associated with any dependent variables after adjustment (Supplemental Table 6, http://links.lww.com/HJH/A789). The effects of SHSE did not change remarkably when study participants were stratified by exposition at home or at work (Supplemental Tables 7–10, http://links.lww.com/HJH/A789).
In this cross-sectional population-based study, we evaluated the relationship between SHSE and conventional cardiovascular risk factors in the general Korean population using the urine cotinine level, a well established, major proximate metabolite of nicotine [20,21]. This study found no significant adverse relationship between any dependent variables and SHSE as assessed by self-report questionnaires. Quantitative assessment of SHSE using urine cotinine levels did not alter this relationship.
There is strong and consistent evidence that SHSE increases the risk of morbidity and mortality, specifically cardiovascular mortality and ischemic heart disease. Studies have shown that SHSE increases the risk of CHD by 25–30% [6,8]. A number of studies have shown that SHSE not only increases the risk of CHD but also impacts morbidity and mortality associated with acute coronary syndrome [22–24]. Several mechanisms have been proposed such as platelet and endothelial dysfunction, increased arterial stiffness, atherosclerosis, increased oxidative stress and inflammation, and decreased energy metabolism . Aside from chronic effects, acute effects have been proposed, including an increase in resting heart rate (HR), BP, blood level of carboxyhemoglobin, and carbon monoxide, and a marked reduction in microcirculatory flow and HR variability [26,27].
Hypertension and dyslipidemia are established cardiovascular risk factors. Active smoking has been shown to have adverse effects on BP and lipid profiles [28–31]. However, for SHSE, there is a paucity of data regarding its association with cardiovascular risk factors. There are a few studies that have shown the association between SHSE and hypertension. Makris et al. found that passive smoking is associated with masked hypertension in a dose-related manner in 790 normotensive nonsmokers who were self-referred to an outpatient hypertensive clinic. Li et al. also found that passive smoking was a significant risk factor for hypertension in 392 Chinese nonsmoking women. Alshaarawy et al. revealed higher SHSE, measured objectively by serum cotinine levels, was associated with SBP and hypertension. Regarding blood lipid levels, a previous study showed deteriorations in lipid profiles with higher cotinine levels among nonsmokers . However, another study found no significant differences according to subjectively assessed SHSE . In summary, there have been limited studies with inconsistent results.
In this study, we failed to find any consistent and meaningful changes in BP, cholesterol, and fasting glucose levels attributable to SHSE. One potential explanation is publication bias. Studies lacking statistically significant associations tend not to be published. Second is a difference in the study population. Previous studies focused on a specific subset of the population, whereas our study participants were from the general population [31–33]. Finally, the source for measuring cotinine levels differed. Urine cotinine levels were used in this study, whereas previous studies measured serum or salivary levels [7,33]. To our knowledge, however, there is no evidence that urinary cotinine measurements are less precise than other measurements [21,34].
There were several findings that were statistically significant in this study. Some were in the opposite direction than what was expected. A higher cotinine level was linked to lower SBP and triglyceride levels. However, those relationships were not consistently observed. For example, triglyceride had a negative relationship with log-transformed cotinine but had no significant relationship with cotinine in quartiles. Subjectively assessed SHSE was associated with higher DBP, whereas objectively assessed SHSE was not. Thus, false positivity that can be caused by multiple testing should be considered.
The current study suggested a possible increase in fasting blood glucose levels with SHSE, although the relationship was not consistent. The relationship was NS with the unadjusted model, but became significant after multivariable adjustment, when cotinine was stratified into quartiles. The linear regression model using logarithm-transformed urine cotinine showed a similar pattern, but the statistical significance was only marginal. Previous studies have also suggested increased risk of type 2 diabetes with SHSE [35,36]. A previous study using the US nationally representative National Health and Nutrition Examination Surveys showed that serum cotinine levels were positively associated with diabetes mellitus . However, the association between serum cotinine levels and glycohemoglobin was NS. The results of this study are potentially hypothesis-generating and need to be tested in future studies.
The finding of no significant association between SHSE and major cardiovascular risk factors may sound contradictory to previous studies. The evidence supporting increased risk of cardiovascular disease and mortality due to SHSE is strong. One explanation is that SHSE may affect cardiovascular disease directly without mediation of major risk factors in the pathway. In addition, the cross-sectional nature of this study needs to be considered. The effects of SHSE on cardiovascular risk factors may be seen only in the long term but not in the short term or cross-sectionally.
The main novelty of our study is that it is the first reported evaluation of the association between conventional cardiovascular risk factors and SHSE using both subjective and objective methods in a large Korean population. We believe that the use of urine cotinine level minimized the potential for misclassification bias that could occur with the use of self-report questionnaires alone.
The main limitation of this study is the cross-sectional nature. Future studies with a longitudinal design would provide better insight into the impact of SHSE on major cardiovascular risk factors. Second, selection bias is possible. Among 37 753 participants who underwent surveys during the study period, 7376 (19.5%) were included in this analysis. In addition, most nonsmokers were women and urine cotinine was less likely to be available in men. Further, more than three-fourths of this study population was woman. Third, although we excluded participants with major cardiovascular disease (i.e., CHD and stroke), there is still a possibility that patients with subclinical cardiovascular disease might have been included in this study. This is another potential source of bias. Forth, the office BP measurements have shown to have lower prognostic value than ambulatory BP monitoring measures [38,39]. Finally, we cannot exclude the possibility that residual confounding and unmeasured confounders are present.
We performed a cross-sectional, population-based study of healthy adults who had never smoked and who were without known cardiovascular diseases with a purpose to evaluate the relationship between SHSE and cardiovascular risk factors. There was no consistent relationship between SHSE measured subjectively by self-report questionnaires and objectively by urine cotinine levels and BP, lipid, or fasting glucose levels. When stratified according to sex and verified by total study population with adjustment of medication, the results did not change. As there has been cumulated evidence that SHSE is associated with an increased risk of cardiovascular mortality and morbidity; this study's findings suggest further long-term prospective studies are needed to evaluate the effect of SHSE as a cardiovascular risk factor.
The authors thank all of the staff involved in this research. The authors are also grateful to Cheol-Ho Kim, MD for his valuable comments and suggestions.
Author contributions: S.K., S.-H.K., and T.-J.Y. were responsible for conception and design of the study. D.H., S.-H.K., H.-J.K., K.-D.H., and I.-Y.O. were contributed to data collection and analysis. J.J.P., Y.C., Y.E.Y., C.-H.Y., J.-W.S., and H.-Y.L. were contributed to data interpretation. Y.-S.C., G.-Y.C., I.-H.C., and D.-J.C. gave advice on the first draft. S.K. and S.-H.K. wrote the first draft of the article. T.-J.Y. accept responsibility for the final content of the article; all of the authors approved the study before submission.
Conflicts of interest
There are no conflicts of interest.
Reviewer's Summary Evaluation
Among contributions, the finding of unexpected lack of significant results for most relations analyzed in the context of limited number of studies on this issue with generally positive results.
Among limitations, the cross-sectional design of the study, which indicate that further prospective studies are needed to clarify this interesting issue.
1. World Health Organization. WHO global report: mortality attributable to tobacco. Geneva: World Health Organization; 2012.
2. Forouzanfar MH, Alexander L, Anderson HR, Bachman VF, Biryukov S, Brauer M, et al. Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks in 188 countries, 1990–2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet
3. Lim SS, Vos T, Flaxman AD, Danaei G, Shibuya K, Adair-Rohani H, et al. A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet
4. Institute of Medicine. Secondhand smoke exposure
and cardiovascular effects, making sense of the evidence. Washington, DC: National Academies Press; 2010.
5. Oberg M, Jaakkola MS, Woodward A, Peruga A, Pruss-Ustun A. Worldwide burden of disease from exposure to second-hand smoke: a retrospective analysis of data from 192 countries. Lancet
6. He J, Vupputuri S, Allen K, Prerost MR, Hughes J, Whelton PK. Passive smoking and the risk of coronary heart disease – a meta-analysis of epidemiologic studies. N Engl J Med
7. Whincup PH, Gilg JA, Emberson JR, Jarvis MJ, Feyerabend C, Bryant A, et al. Passive smoking and risk of coronary heart disease and stroke: prospective study with cotinine measurement. BMJ
8. Dunbar A, Gotsis W, Frishman W. Second-hand tobacco smoke and cardiovascular disease risk: an epidemiological review. Cardiol Rev
9. Alshaarawy O, Xiao J, Shankar A. Association of serum cotinine levels and hypertension in never smokers. Hypertension
10. Kim Y, Choi YJ, Oh SW, Joh HK, Kwon H, Um YJ, et al. Discrepancy between self-reported and urine-cotinine verified smoking status among Korean male adults: analysis of health check-up data from a single private hospital. Korean J Fam Med
11. Aurrekoetxea JJ, Murcia M, Rebagliato M, Lopez MJ, Castilla AM, Santa-Marina L, et al. Determinants of self-reported smoking and misclassification during pregnancy, and analysis of optimal cut-off points for urinary cotinine: a cross-sectional study. BMJ Open
12. Florescu A, Ferrence R, Einarson T, Selby P, Soldin O, Koren G. Methods for quantification of exposure to cigarette smoking and environmental tobacco smoke: focus on developmental toxicology. Ther Drug Monit
13. Cronin EM, Kearney PM, Kearney PP, Sullivan P, Perry IJ. Impact of a national smoking ban on hospital admission for acute coronary syndromes: a longitudinal study. Clin Cardiol
14. Kweon S, Kim Y, Jang MJ, Kim Y, Kim K, Choi S, et al. Data resource profile: the Korea National Health and Nutrition Examination Survey
(KNHANES). Int J Epidemiol
15. Kim S, Jung A. Optimum cutoff value of urinary cotinine distinguishing South Korean adult smokers from nonsmokers using data from the KNHANES (2008–2010). Nicotine Tob Res
16. Kang YH, Lee YJ, Kim HK, Yun YH, Jeong SY, Lee JS, Park JG. Usefulness of urinary cotinine test to distinguish smokers from nonsmokers. Korean J Lab Med
17. SRNT Subcommittee on Biochemical Verification. Biochemical verification of tobacco use and cessation. Nicotine Tob Res
18. The Fifth Korea National Health and Nutrition Examination Survey
(KNHANES V), 2011 Health Examination. Cheongju: Korea Centers for Disease Control and Prevention; 2011. https://knhanes.cdc.go.kr/knhanes/index.do
(Accessed 18 March 2017).
19. Jung S, Lee IS, Kim SB, Moon CS, Jung JY, Kang YA, et al. Urine cotinine
for assessing tobacco smoke exposure in Korean: analysis of the Korea National Health and Nutrition Examination Survey
(KNHANES). Tuberc Respir Dis (Seoul)
20. Jarvis MJ, Russell MA, Benowitz NL, Feyerabend C. Elimination of cotinine from body fluids: implications for noninvasive measurement of tobacco smoke exposure. Am J Public Health
21. Benowitz NL. Cotinine as a biomarker of environmental tobacco smoke exposure. Epidemiol Rev
22. Panagiotakos DB, Pitsavos C, Stefanadis C. Chronic exposure to second hand smoke and 30-day prognosis of patients hospitalised with acute coronary syndromes: the Greek study of acute coronary syndromes. Heart
23. Pell JP, Haw S, Cobbe S, Newby DE, Pell AC, Fischbacher C, et al. Secondhand smoke exposure
and survival following acute coronary syndrome: prospective cohort study of 1261 consecutive admissions among never-smokers. Heart
24. Notara V, Panagiotakos DB, Kouroupi S, Stergiouli I, Kogias Y, Stravopodis P, et al. Smoking determines the 10-year (2004–2014) prognosis in patients with Acute Coronary Syndrome: the GREECS observational study. Tob Induc Dis
25. Barnoya J, Glantz SA. Cardiovascular effects of secondhand smoke: nearly as large as smoking. Circulation
26. Henriksson P, Lu Q, Diczfalusy U, Freyschuss A. Immediate effect of passive smoking on microcirculatory flow. Microcirculation
27. Yarlioglues M, Kaya MG, Ardic I, Calapkorur B, Dogdu O, Akpek M, et al. Acute effects of passive smoking on blood pressure
and heart rate in healthy females. Blood Press Monit
28. Bowman TS, Gaziano JM, Buring JE, Sesso HD. A prospective study of cigarette smoking and risk of incident hypertension in women. J Am Coll Cardiol
29. Ge P, Dong C, Ren X, Weiderpass E, Zhang C, Fan H, et al. The high prevalence of low HDL-cholesterol levels and dyslipidemia in rural populations in northwestern China. PLoS One
30. Erhardt L. Cigarette smoking: an undertreated risk factor for cardiovascular disease. Atherosclerosis
31. Li N, Li Z, Chen S, Yang N, Ren A, Ye R. Effects of passive smoking on hypertension in rural Chinese nonsmoking women. J Hypertens
32. Makris TK, Thomopoulos C, Papadopoulos DP, Bratsas A, Papazachou O, Massias S, et al. Association of passive smoking with masked hypertension in clinically normotensive nonsmokers. Am J Hypertens
33. Lu L, Mackay DF, Newby DE, Pell JP. Association between salivary cotinine and cardiovascular biomarkers among nonsmokers and current smokers: cross-sectional study of 10,081 participants. Eur J Vasc Endovasc Surg
34. Benowitz NL, Dains KM, Dempsey D, Herrera B, Yu L, Jacob P 3rd. Urine nicotine metabolite concentrations in relation to plasma cotinine during low-level nicotine exposure. Nicotine Tob Res
35. Wang Y, Ji J, Liu YJ, Deng X, He QQ. Passive smoking and risk of type 2 diabetes: a meta-analysis of prospective cohort studies. PLoS One
36. Pan A, Wang Y, Talaei M, Hu FB, Wu T. Relation of active, passive, and quitting smoking with incident type 2 diabetes: a systematic review and meta-analysis. Lancet Diabetes Endocrinol
37. Alshaarawy O, Elbaz HA. Serum cotinine levels and diabetes mellitus in never smokers. J Diabetes Complications
38. Clement DL, De Buyzere ML, De Bacquer DA, de Leeuw PW, Duprez DA, Fagard RH, et al. Prognostic value of ambulatory blood-pressure recordings in patients with treated hypertension. N Engl J Med
39. Sebo P, Pechere-Bertschi A, Herrmann FR, Haller DM, Bovier P. Blood pressure
measurements are unreliable to diagnose hypertension in primary care. J Hypertens
* Sehun Kim and Si-Hyuck Kang contributed equally to the article.