Lipoprotein-associated phospholipase A2 (Lp-PLA2) has been reported as a sensitive biomarker for cardiovascular disease. However, the association of La-PLA2 and atherosclerotic markers are now always warranted. The V279F loss-of-function variant of PLA2G7 gene encoding Lp-PLA2 is commonly seen in East Asians. To clarify the association of Lp-PLA2 and atherosclerotic markers, the PLA2G7 V279F genotypes in the general Japanese populations were investigated.
Design and Method:
Serum triglyceride, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C), systolic and diastolic blood pressure (SBP and DBP), and atherosclerosis indices such as brachial-ankle pulse wave velocity (baPWV), ankle-brachial index (ABI) and carotid intima-media thickness (CIMT) were assessed in 792 participants (385 men, 64.1 ± 9.0 years; 407 women, 62.6 ± 9.5 years). Rs 76863441 polymorphism (exon 9, G994T) was screened using a TaqMan allelic discrimination assay. Multivariate regression analyses and analyses of covariance adjusted for age, BMI, medication, alcohol consumption, smoking and walking status were carried.
Significant association of V279F genotype with triglyceride (β=0.15, P = 0.005) and with ABI (β = 0.12, P = 0.036) were seen in men. It also showed trend of being associated with HDL-C in men (β =− 0.09, P = 0.080) and with baPWV in women (β = 0.08, P = 0.080). Men with rare T allele showed significantly higher triglyceride (P = 0.004) compared to those in men with GG wild type, and women with T allele showed significantly higher baPWV (P = 0.011) compared with those in women with GG wild type.
Lp-PLA2 V279F polymorphisms were significantly associated with atherosclerotic markers in the general Japanese population with gender differences.