Original Papers: PDF OnlyIntrarenal Adenosine Produces Hypertension by Activating the Sympathetic Nervous System via the Renal Nerves In the DogKatholi, Richard E.; Whitlow, Patrick L.; Hageman, Gilbert R.; Woods, Thomas W. Author Information From the Departments of Medicine and Physiology/Biophysics, University of Alabama in Birmingham, Birmingham, Alabama 35294, USA. Sponsorship: This work was supported by the American Heart Association, Alabama Affiliate (790018) and by the National Heart, Lung and Blood Institute (HL 24420). Drs Katholi and Hageman are Established Investigators of the American Heart Association Norwich-Eaton Pharmaceutical Company Supplied Sar1 Ala8 angiotensin II. Requests for reprints to: Dr Richard E. Katholi, Department of Medicine, University of Alabama in Birmingham, Birmingham, Alabama 35294, USA. Date of receipt: 17 November 1983; revised: 8 March 1984. Journal of Hypertension 2(4):p 349-359, August 1984. Buy Abstract Studies from our laboratory suggest that the afferent renal nerves from the clipped kidney enhance sympathetic nervous system activity in established one-kidney, one clip and two-kidney, one clip Goldblatt hypertension. Because adenosine is released during renal ischaemia and adenosine has been shown to increase the frequency of afferent renal nerve signals, we proposed the hypothesis that intrarenal adenosine might produce hypertension by activating the sympathetic nervous system via the afferent renal nerves. To examine this hypothesis, changes in arterial pressure and activity of the sympathetic nervous system were measured during renal artery infusion of adenosine before and after renal denervation in uninephrectomized sodium replete conscious dogs. Intrarenal adenosine infusion produced a 21 ± 3 mmHg mean arterial pressure rise in association with an increase in plasma norepinephrine. Ganglionic blockade during intrarenal adenosine infusion resulted in a significantly greater decrease in arterial pressure compared to control responses. After renal denervation, intrarenal adenosine infusion resulted in no change in arterial pressure, plasma norepinephrine or arterial pressure response to ganglionic blockade. To further assess sympathetic activity changes, right renal norepinephrine secretion and multifibre efferent neural traffic were measured during left renal artery adenosine infusion in α-chloralose-anaesthetized dogs. Left renal artery adenosine infusion resulted in increased right renal vascular resistance in association with increased renal norepinephrine secretion and increased efferent neural activity. The data indicate that in the dog with intact renal nerves, intrarenal adenosine produces hypertension by activating the sympathetic nervous system. Journal of Hypertension 2:349-359, 1984 © Lippincott-Raven Publishers.