Secondary Logo

Journal Logo


Corneal lenticule extraction assisted by a low-energy femtosecond laser

Izquierdo, Luis Jr MD, PhD; Sossa, Daniel MD; Ben-Shaul, Or MD; Henriquez, Maria A. MD, PhD

Author Information
Journal of Cataract & Refractive Surgery: September 2020 - Volume 46 - Issue 9 - p 1217-1221
doi: 10.1097/j.jcrs.0000000000000236
  • Free
  • SDC
  • Associated Video


For the past 2 decades, laser-assisted in situ keratomileusis (LASIK) has become the standard procedure for corneal refractive surgery. More recently, small-incision lenticule extraction (SMILE) using the femtosecond laser has gained widespread acceptance.1–5 Furthermore, SMILE has been reported to be comparable with femtosecond laser–assisted LASIK (FS-LASIK) for safety, efficacy, and predictability.1–5

SMILE was introduced in 2011 for the treatment of myopia and myopic astigmatism; it is a flapless technique in which a stromal lenticule is extracted through a small incision (2.0 to 5.0 mm).6 The conventional technique of SMILE involves docking, femtosecond laser application, lenticule dissection from the surrounding stroma, and extraction.6–8

The advantage of this minimally invasive approach is maintaining integrity of the anterior cornea and protecting the subbasal innervation. Moreover, studies have suggested that using a SMILE technique resulted in better ocular surface stability and biomechanical strength when compared with FS-LASIK.9–12 Despite the fact that SMILE has been proven a safe technique, it is not free of complications and has the disadvantage of a long learning curve for the surgeon when compared with conventional FS-LASIK procedures.13–17

The SMILE procedure requires greater surgical skill when compared with other refractive surgeries because the lenticule is manually removed through a small incision.13–16 Removing the stromal lenticule in 1 piece can sometimes be challenging, especially in patients with low myopia and a thin lenticule.18,19 Difficult lenticule dissection and extraction is the most common complication encountered during the initial learning curve, with an incidence of up to 16%.13 Identifying the lenticule edge at the beginning of the surgery is crucial to ensure lamellar separation in the correct plane and to prevent lenticule misdissection.13–16

In a recent retrospective study, intraoperative complications were reported in 4.46% of cases, including suction loss, an opaque bubble layer, and tearing of the lenticule.13 For this reason, different modifications of the surgical technique have been described to ease the process of lenticule extraction and minimize complications, such as Chung's swing technique, lenticulerhexis, lenticuloschisis, hydroexpression, and lenticule extraction guided by optical coherence tomography.20–24 Several factors have been associated with early visual recovery after SMILE, including scanning pattern,25 laser parameters (eg, spot distance and energy setting), and surgical skills.26–29 Therefore, optimizing the laser parameters and modifying the surgical technique might improve postoperative visual acuity and recovery time. Suction loss is more commonly observed after SMILE because it is a low-pressure system (intraocular pressure approximately 35 mm Hg) with a longer suction time, when compared with FS-LASIK.17 Unintended posterior plane dissection and tearing of the lenticule leads to difficulty of correctly identifying the anterior plane of the lenticule.16

At the time of this study, the VisuMax Laser System (Carl Zeiss Meditec AG) was the only U.S. Food and Drug Administration–approved laser platform for the creation of an intrastromal lenticule. In addition to the VisuMax Laser System, the platform used in this study, the FEMTO LDV Z8 (Ziemer Ophthalmic Systems AG), received the Conformité Européenne mark. The FEMTO LDV Z8 is based on a low-energy concept, where the miniaturized scanning optic, integrated into the handpiece and its high numerical aperture, creates highly focused laser pulses achieving photodisruption in the low nanojoules range (<100 nJ). Thus, the advantages of the low-energy concept are the decreased stromal gas generation and an accurate laser focus. The repetition rate is of high frequency, in the range of several megahertz. Therefore, the pulses are not at spot distance from each other but are instead overlapping. The laser pulses are guided from the laser source through an articulated moveable arm to a handpiece that is adaptable in position and height with a very close working distance to the eye. In addition, the FEMTO LDV Z8 offers the possibility of recentering the treatment area after having performed the docking, which is not possible with the VisuMax Laser System.30 The aim of this study was to describe and evaluate a new technique for lenticule extraction using a low-energy femtosecond laser, the FEMTO LDV Z8.


The study followed the tenets of the Declaration of Helsinki, with the local institutional review board providing ethics approval (No. IRB00003251). All surgeries were conducted by the same surgeon (L.I.) between February 2019 and May 2019.

Preoperatively, the visual axis was marked by using the slitlamp. First, after a blink, a small slit was adjusted to the first Purkinje reflex. Second, the slit was widened on the horizontal axis and marked with ink at the limbus. Third, in the same manner, perpendicular to the horizontal axis, the vertical axis was marked (Figure 1).

Figure 1.
Figure 1.:
Marking of the visual axis (purple).

After placing the patients on the surgical bed and administering topical anesthesia, patients were instructed to fixate on the fixation light to ensure accurate centration, and suction was initiated. However, if the surgeon was not satisfied with the centration of the docking, the femtosecond laser allowed recentering of the treatment area, including correction of cyclotorsion by manipulation on the monitor (Figure 2, A) without having to release the suction and start the docking procedure again. The 2 small incisions of 3.0 mm width were cut separately at 35-degree and 145-degree positions with an entrance angle of 90 degrees; each incision allowed for the posterior and anterior surfaces of the lenticule to be delineated directly and independently. The surgeon could adjust the distance between the incisions and the angle position for greater comfort and could decide if 1 or both incisions were cut and used. In all cases, the lenticule had a diameter of 6.5 mm without adding any additional thickness. During cutting of the lenticule, the applied vacuum level to the eye was 700 mbar, the same as used for LASIK flaps.

Figure 2.
Figure 2.:
Guided lenticule extraction technique. A: Planning screen and centration screen (before cutting). Planed incisions (blue) and centration lines (red), including correction of cyclotorsion, are shown. The blurred circle represents the size of the planned lenticule. B: After cutting the lenticule, the left incision (B) and the right incision (C) were checked, and the anterior and posterior planes, respectively, were identified. Separation of the anterior plane (D) and posterior plane (E) was done by swiping the tool left and right in a circular manner. F: The lenticule was grabbed and pushed out through the other incision. This step was followed by a visual check of the lenticule integrity and a corneal massage.

After laser application (Figures 2, B and C), first incision guiding to the anterior plane was opened using a small pointed spatula (Duckworth & Kent Ltd.), delineating the anterior edge of the lenticule. Then, the incision guiding to the posterior plane was opened in the same manner disconnecting the posterior edge of the lenticule. The anterior interface was then separated using a smooth spoon-shaped tip dissector (Duckworth & Kent Ltd.), and the posterior lenticular interface was separated in a similar fashion (Figures 2, D and E). Once both planes had been separated, the lenticule was removed from the cornea by grasping the edge of the lenticule or pushing it through the opposite incision (Figure 2, F) with a 23-gauge microforceps (Duckworth & Kent Ltd.). The technique is shown in Video 1 (see Supplemental Digital Content, available at After observing the integrity of the extracted lenticule on the cornea, the stromal bed was flushed with a balanced salt solution. Finally, massage was applied to the cornea.


The guided lenticule extraction technique was performed in 5 eyes of 5 patients: 3 women and 2 men. The mean age was 28.4 ± 3.9 years, and the mean sphere, cylinder, and spherical equivalents were −3.05 ± 0.48 diopters (D), 0.50 ± 0.25 D, and −3.30 ± 0.57 D, respectively. All patients had a remaining corneal residual bed thickness of 300 µm or more, measured by corneal tomography (Galilei G6, Ziemer Ophthalmic Systems AG). Table 1 summarizes the preoperative and postoperative visual acuities and refraction of the patients.

Table 1.
Table 1.:
Preoperative and postoperative visual acuity and refractive values of the patients.
Table 1-A.
Table 1-A.:
Preoperative and postoperative visual acuity and refractive values of the patients.

A complete dissection and removal of the lenticule was achieved in all cases without any intraoperative complications, and at postoperative day 1, all patients had a clear cornea, as shown in Figure 3. In the postoperative period, 1 patient presented with a mild stromal haze in the interface that resolved with 2 weeks of topical corticosteroids treatment 4 times a day (prednisolone acetate 1%; Pred Forte, Allergan). No other complications were observed during the follow-up period.

Figure 3.
Figure 3.:
Evolution of a patient's right eye 24 hours postoperatively. A: Final result after emission of the low-energy femtosecond laser; 2 small incisions separated, and the left incision allowed the surgeon to delineate and dissect the anterior surface of the lenticule, whereas the right incision allowed the separation of the posterior surface of the lenticule. B: 24 hours postoperatively, the patient's cornea was completely clear. C: Upper and lower edges of the circumferential incision. D: The edge of the right incision of 3.0 mm width.


In this study, we reported for the first time, to our knowledge, a new guided lenticule extraction technique, using 2 small incisions of 3.0 mm that delineate directly the anterior and posterior edges of the lenticule, saving one of the most difficult and important steps of the standard technique. In all the patients, lenticule removal was complete without any intraoperative complications.

We believe that another important advantage of this technique is the ability to recenter the treatment area when necessary, without releasing the suction and starting a new docking procedure, permitting perfect centering that is less traumatic for the patient. It remains unclear whether our findings can be generalized to a general population. Therefore, further studies should investigate the safety and efficacy of the proposed technique. We believe that guided lenticule extraction with the low-energy femtosecond laser has the potential to shorten the learning curve for surgeons and lower the complication rate of lenticular extractions.


  • Small-incision lenticule extraction has been proven to be an effective and a safe technique, comparable with LASIK regarding visual acuity but requires a long learning curve.
  • Of the complications related to lenticule extraction, the difficulty in identifying the anterior and posterior surfaces of the lenticule is one of the main causes of complications in small-incision lenticule extraction.


  • Low-energy femtosecond laser technology was used for guided lenticule extraction, which allowed for centering of the treatment area without having to repeat docking.
  • Guided lenticule extraction used 2 incisions that allowed for easy identification and separation of the anterior and posterior surfaces of the lenticule independently.


1. Lin F, Xu Y, Yang Y. Comparison of the visual results after SMILE and femtosecond laser-assisted LASIK for myopia. J Refract Surg 2014;30:248–254
2. Sekundo W, Gertnere J, Bertelmann T, Solomatin I. One-year refractive results, contrast sensitivity, high-order aberrations and complications after myopic small-incision lenticule extraction (ReLEx SMILE). Graefe's Archive Clin Exp Ophthalmol 2014;252:837–843
3. Zhang Y, Shen Q, Jia Y, Zhou D, Zhou J. Clinical outcomes of SMILE and FS-LASIK used to treat myopia: a meta-analysis. J Refract Surg 2016;32:256–265
4. Kamiya K, Shimizu K, Igarashi A, Kobashi H. Visual and refractive outcomes of femtosecond lenticule extraction and small-incision lenticule extraction for myopia. Am J Ophthalmol 2014;157:128–134.e1
5. Blum M, Täubig K, Gruhn C, Sekundo W, Kunert KS. Five-year results of small incision lenticule extraction (ReLEx SMILE). Br J Ophthalmol 2016;100:1192–1195
6. Sekundo W, Kunert KS, Blum M. Small incision corneal refractive surgery using the small incision lenticule extraction (SMILE) procedure for the correction of myopia and myopic astigmatism: results of a 6 month prospective study. Br J Ophthalmol 2011;95:335–339
7. Shah R, Shah S, Sengupta S. Results of small incision lenticule extraction: all-in-one femtosecond laser refractive surgery. J Cataract Refract Surg 2011;37:127–137
8. Ivarsen A, Hjortdal J. Correction of myopic astigmatism with small incision lenticule extraction. J Refract Surg 2014;30:240–247
9. Kobashi H, Kamiya K, Shimizu K. Dry eye after small incision lenticule extraction and femtosecond laser–assisted LASIK: meta-analysis. Cornea 2016;36:85–91
10. Cai WT, Liu QY, Ren CD, Wei QQ, Liu JL, Wang QY, Du YR, He MM, Yu J. Dry eye and corneal sensitivity after small incision lenticule extraction and femtosecond laser-assisted in situ keratomileusis: a meta-analysis. Int J Ophthalmol 2017;10:632
11. Li M, Zhou Z, Shen Y, Knorz MC, Gong L, Zhou X. Comparison of corneal sensation between small incision lenticule extraction (SMILE) and femtosecond laser-assisted LASIK for myopia. J Refract Surg 2014;30:94–100
12. Wu D, Wang Y, Zhang L, Wei S, Tang X. Corneal biomechanical effects: small-incision lenticule extraction versus femtosecond laser–assisted laser in situ keratomileusis. J Cataract Refract Surg 2014;40:954–962
13. Titiyal JS, Kaur M, Rathi A, Falera R, Chaniyara M, Sharma N. Learning curve of small incision lenticule extraction: challenges and complications. Cornea 2017;36:1377–1382
14. Ivarsen A, Asp S, Hjortdal J. Safety and complications of more than 1500 small-incision lenticule extraction procedures. Ophthalmology 2014;121:822–828
15. Ramirez-Miranda A, Ramirez-Luquin T, Navas A, Graue-Hernandez EO. Refractive lenticule extraction complications. Cornea 2015;34:S65–S67
16. Wang Y, Ma J, Zhang J, Dou R, Zhang H, Li L, Zhao W, Wei P. Incidence and management of intraoperative complications during small-incision lenticule extraction in 3004 cases. J Cataract Refract Surg 2017;43:796–802
17. Wong CW, Chan C, Tan D, Mehta JS. Incidence and management of suction loss in refractive lenticule extraction. J Cataract Refract Surg 2014;40:2002–2010
18. Reinstein DZ, Archer TJ, Gobbe M, Johnson N. Accuracy and reproducibility of Artemis central flap thickness and visual outcomes of LASIK with the Carl Zeiss Meditec VisuMax femtosecond laser and MEL 80 excimer laser platforms. J Refract Surg 2010;26:107–119
19. Shetty R, Negalur N, Shroff R, Deshpande K, Jayadev C. Cap lenticular adhesion during small incision lenticular extraction surgery: causative factors and outcomes. Asia Pac J Ophthalmol (Phila) 2017;6:233–237
20. Kim BK, Mun SJ, Lee DG, Choi HT, Chung YT. Chung’s swing technique: a new technique for small-incision lenticule extraction. BMC Ophthalmol 2016;16:154
21. Zhao Y, Li M, Yao P, Shah R, Knorz MC, Zhou X. Development of the continuous curvilinear lenticulerrhexis technique for small incision lenticule extraction. J Refract Surg 2015;31:16–21
22. Ganesh S, Brar S. Lenticuloschisis: a “no dissection” technique for lenticule extraction in small incision lenticule extraction. J Refract Surg 2017;33:563–566
23. Ng AL, Cheng GP, Woo VC, Jhanji V, Chan TC. Comparing a new hydroexpression technique with conventional forceps method for SMILE lenticule removal. Br J Ophthalmol 2018;102:1122–1126
24. Sharma N, Urkude J, Chaniyara M, Titiyal JS. Microscope-integrated intraoperative optical coherence tomography-guided small-incision lenticule extraction: new surgical technique. J Cataract Refract Surg 2017;43:1245–1250
25. Shah R, Shah S. Effect of scanning patterns on the results of femtosecond laser lenticule extraction refractive surgery. J Cataract Refract Surg 2011;37:1636–1647
26. Li L, Schallhorn JM, Ma J, Cui T, Wang Y. Energy setting and visual outcomes in SMILE: a retrospective cohort study. J Refract Surg 2018;34:11–16
27. Kunert KS, Blum M, Duncker GI, Sietmann R, Heichel J. Surface quality of human corneal lenticules after femtosecond laser surgery for myopia comparing different laser parameters. Graefes Archive Clin Exp Ophthalmol 2011;249:1417–1424
28. Ji YW, Kim M, Kang DSY, Reinstein DZ, Archer TJ, Choi JY, Kim EK, Lee HK, Seo KY. Lower laser energy levels lead to better visual recovery after small-incision lenticule extraction: prospective randomized clinical trial. Am J Ophthalmol 2017;179:159–170
29. Donate D, Thaëron R. Lower energy levels improve visual recovery in small incision lenticule extraction (SMILE). J Refract Surg 2016;32:636–642
30. Pajic B, Cvejic Z, Pajic-Eggspuehler B. Cataract surgery performed by high frequency LDV Z8 femtosecond laser: safety, efficacy, and its physical properties. Sensors 2017;17:1429
Copyright © 2020 Published by Wolters Kluwer on behalf of ASCRS and ESCRS