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In Vitro Effect of Bromelain on the Regenerative Properties of Mesenchymal Stem Cells

Ghensi, Paolo, DDS, Clin MSc*,†,‡; Cucchi, Alessandro, DDS, PhD§; Bonaccorso, Antonio, MD, DDS; Ferroni, Letizia, PhD||; Gardin, Chiara, PhD||; Mortellaro, Carmen, MD, DDS**; Zavan, Barbara, PhD||,††

Journal of Craniofacial Surgery: June 2019 - Volume 30 - Issue 4 - p 1064–1067
doi: 10.1097/SCS.0000000000004862
Clinical Studies

Background: Bromelain belongs to a group of protein-digesting enzymes obtained commercially from the fruit or stem of pineapple. Several studies demonstrated that bromelain exhibits various fibrinolytic, anti-edematous, antithrombotic, and anti-inflammatory activities supporting its application for many therapeutic benefits. The aim of this study was to analyze the effects of bromelain on the pro-wound healing activities and the regenerative properties of mesenchymal stem cells.

Methods: Mesenchymal stem cells were treated in vitro with bromelain alone or combined with dexamethasone sodium phosphate. Real-time polymerase chain reaction was performed to profile the expression of extracellular matrix components and remodeling enzymes, and cytokines.

Results: The combination of bromelain and dexamethasone sodium phosphate induced a great activation of mesenchymal stem cells with an increase in hyaluronan and collagen production and anti-inflammatory cytokines release.

Conclusion: Based on the results of this in vitro study, the combined use of bromelain and dexamethasone sodium phosphate stimulated the pro-wound healing activities and the regenerative properties of mesenchymal stem cells better than bromelain and dexamethasone alone.

*Centre for Integrative Biology (CIBIO), University of Trento, Trento

Institute for Dental and Implant Studies (IDIS)

Department of Neurosciences, University of Padova, Padova

§Department of Biomedical and Neuromotorial Science, University of Bologna, Bologna

Private Practice, Merano

||Department of Biomedical Sciences, University of Padova, Padova

**Department of Health Sciences, “A. Avogadro” University of Eastern Piedmont, Novara

††Maria Cecilia Hospital, GVM Care and Research, Cotignola (RA), Italy.

Address correspondence and reprint requests to Paolo Ghensi, DDS, Clin MSc, Via Chini 101/2, 38123 Trento (TN), Italy; E-mail:

Received 29 April, 2018

Accepted 11 June, 2018

The authors report no conflicts of interest.

© 2019 by Mutaz B. Habal, MD.