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Fabrication of Poly(L-Lactic Acid)/Chitosan Scaffolds by Solid–Liquid Phase Separation Method for Nerve Tissue Engineering

An In Vitro Study on Human Neuroblasts

Salehi, Majid, PhD*,†; Farzamfar, Saeed; Bozorgzadeh, Shaghayegh, DDS§; Bastami, Farshid, DDS§,||

Journal of Craniofacial Surgery: May 2019 - Volume 30 - Issue 3 - p 784–789
doi: 10.1097/SCS.0000000000005398
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Polymeric scaffolds that support neural cell behaviors are attracting more attention. In the present study, solid–liquid phase separation technique is used to fabricate scaffolds made of poly(L-lactic acid) (PLLA) and chitosan (CS) blends to mimic both cellular microenvironment and anatomical structure of nerve tissue. The fabricated scaffolds favor characteristics of both natural and synthetic polymers. Different tests and assays including physical and mechanical ones (in vitro degradation rate, free radical release, hydrophilicity, and porosity measurements, microstructure observation, and mechanical tests) and cellular assays (cell attachment measurement and viability assessment) suggest that blend scaffolds prepared with this method support nerve cells for tissue engineering applications adequately and even better than scaffolds prepared with the same method but from pure PLLA or CS.

*Department of Tissue Engineering

Tissue Engineering and Stem Cell Research Center, Shahroud University of Medical Sciences, Shahroud

Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences

§Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences

||Dental Reseach Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Address correspondence and reprint requests to Farshid Bastami, DDS, Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran 1983963113, Iran; E-mail: farshidbastami@sbmu.ac.ir

Received 2 January, 2018

Accepted 10 January, 2019

The authors report no conflicts of interest.

Supplemental digital contents are available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.jcraniofacialsurgery.com).

© 2019 by Mutaz B. Habal, MD.