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Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering

Boeckel, Daniel Goncalves, DDS, PhD*; Sesterheim, Patrícia, DMSc, PhD; Peres, Thiago Rodrigues, DDS; Augustin, Adolpho Herbert, DDS; Wartchow, Krista Minéia, DMSc§; Machado, Denise Cantarelli, DMSc, PhD||; Fritscher, Guilherme Genehr, DDS, PhD; Teixeira, Eduardo Rolim, DDS, PhD*

Journal of Craniofacial Surgery: May 2019 - Volume 30 - Issue 3 - p 777–783
doi: 10.1097/SCS.0000000000005392
Original Articles

This study investigates the applicability of adipose mesenchymal stem cells (mADSCs) and hyaluronic acid (HA) as a cellular compound for bone tissue engineering. A critical bone defect was created on each femur of 25 rats in vivo, receiving the following 5 graft treatments: I—Control-defect; II—HA; III—mADSCs; IV—mADSCs+HA; and V—previously osteoinduced mADSCs+HA. Evaluation using microcomputed tomography, histomorphometry, and RT-PCR analysis was performed 23 days after implantation. Microcomputed tomography analysis indicated higher means of bone contact surface (BCS) and bone surface density (BSD) for the mADSCs+HA group compared with Control and the HA groups (P < 0.05). Histomorphometric findings showed higher means of bone regeneration in the mADSCs+HA compared with HA and Control groups (P < 0.05). The RT-PCR ratios showed no difference in type 1 collagen (Col1A) gene expression or osteopontin (OP) gene expression, whereas for the osteonectin gene (ON) higher means were found in the HA and mADSCs osteoin+HA groups (P < 0.05). These results suggest that a combination of HA and mADSCs without prior osteoinduction might be applicable for bone tissue regeneration.

*Department of Dental Prosthesis, Pontifical Catholic University of Rio Grande do Sul – PUCRS

Experimental Cardiology Center, Institute of Cardiology

Institute of Petroleum and Natural Resources – PUCRS

§Department of Biochemistry, Federal University of Rio Grande do Sul – UFRGS

||Laboratory of Cell Biology and Respiratory Diseases

Department of Oral Surgery, Pontifical Catholic University of Rio Grande do Sul – PUCRS, Porto Alegre, Brazil.

Address correspondence and reprint requests to Daniel Goncalves Boeckel, DDS, PhD, Av. Idependencia 925/1001, Independencia, Porto Alegre, RS, 98915-000, Brazil; E-mail:

Received 4 October, 2018

Accepted 4 January, 2019

The authors report no conflicts of interest.

© 2019 by Mutaz B. Habal, MD.